Impact of the Smoking Process on Biogenic Amine Levels in Traditional Dry-Cured Chorizo
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chorizo Technology and Sampling Procedures
2.2. Physicochemical Analyses
2.3. Sample Preparation and BA Extraction
2.4. Chromatographic Analysis by LC-DAD-ESI/MSn
2.5. Chromatographic Analysis by HPLC-DAD
2.6. Statistical Analysis
3. Results and Discussion
3.1. Biogenic Amine Identification
3.2. Biogenic Amine Quantification by HPLC-DAD
3.3. Statistical Analysis
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Parameter | Value |
---|---|
Water activity (aw) | 0.94 |
Salt content | 3.2 g 100 g−1 |
Benzo(a)pyrene | 0.70 µg kg−1 |
Nitrates | <68 mg NaNO3 kg−1 |
Nitrites | <11 mg NaNO2 kg−1 |
Derivatized BA * | Rt (min) | λmax (nm) | [M + H]+ | MS2 (rel.int.) |
---|---|---|---|---|
MET + 1DNS | 9.29 | 239, 332 | 265.3 | 250.0 (100%), 171.2 (50%) |
Ethylamine + 1DNS | 11.61 | 239, 335 | 279.2 | 263.9 (100%), 157.2 (90%) |
Dimethylamine + 1DNS | 15.05 | 239, 320 | 279.2 | 264.0 (100%), 157.3 (70%) |
PHE + 1DNS | 23.43 | 245, 335 | 355.3 | 157.2 (100%), 339.9 (70%) |
ISO-PEN + 1DNS | 24.62 | 239, 335 | 321.3 | 157.2 (100%), 306.0 (30%) |
PUT + 2DNS | 26.22 | 221, 251 | 555.4 | 304.0 (100%), 220.1 (90%) |
CAD + 2DNS | 28.48 | 224, 251, 335 | 569.4 | 318.1 (100%), 169.2 (90%) |
TYR + 2DNS | 38.43 | 227, 248, 344 | 604.3 | 370.0 (100%), 171.1 (40%) |
Batch | Type of Production | Stage of the Filling Process | MET | PHE | ISO-PEN | PUT | CAD | TYR | Total |
---|---|---|---|---|---|---|---|---|---|
A | Non-smoked | Beginning | 0.213 ± 0.066 | 1.14 ± 0.12 | ND | 26.3 ± 9.7 | 261 ± 28 | 88.0 ± 8.2 | 377 ± 45 |
End | 0.241 ± 0.057 | ND | ND | ND | 52.2 ± 1.7 | 17.7 ± 1.7 | 70.1 ± 0.5 | ||
Smoked | Beginning | 0.489 ± 0.026 | 14.9 ± 1.4 | 1.91 ± 0.07 | 372 ± 85 | 570 ± 83 | 281 ± 29 | 1239 ± 192 | |
End | 0.488 ± 0.169 | 0.689 ± 0.255 | 1.62 ± 0.07 | 181 ± 27 | 263 ± 43 | 230 ± 50 | 677 ± 116 | ||
B | Non-smoked | Beginning | 0.274 ± 0.053 | 0.209 ± 0.116 | ND | ND | ND | ND | 0.482 ± 0.159 |
End | 0.182 ± 0.057 | ND | ND | ND | 36.9 ± 10.1 | 19.3 ± 3.0 | 56.4 ± 12.9 | ||
Smoked | Beginning | 0.524 ± 0291 | 36.4 ± 2.9 | 1.89 ± 0.53 | 1372 ± 70 | 261 ± 68 | 199 ± 64 | 1871 ± 147 | |
End | 0.698 ± 0.101 | 33.9 ± 1.4 | 2.20 ± 0.20 | 837 ± 113 | 701 ± 159 | 175 ± 41 | 1749 ± 307 |
MET | PHE | ISO-PEN | PUT | CAD | TYR | Total | |
---|---|---|---|---|---|---|---|
p-values | |||||||
Batch | 0.481 | <0.05 * | 0.704 | <0.05 * | 0.582 | 0.720 | 0.289 |
Type | <0.05 * | <0.05 * | <0.05 * | <0.05 * | <0.05 * | <0.05 * | <0.05 * |
Stage of filling | 0.760 | 0.464 | 0.958 | 0.387 | 0.981 | 0.524 | 0.454 |
MET | PHE | ISO-PEN | PUT | CAD | TYR | Total | ||
---|---|---|---|---|---|---|---|---|
p-values | ||||||||
Type | <0.001 * | <0.001 * | <0.001 * | <0.001 * | <0.001 * | <0.001 * | <0.001 * | |
Stage of filling | 0.589 | 0.260 | 0.814 | 0.238 | 0.974 | 0.297 | 0.108 | |
Type × Stage of filling | 0.239 | 0.362 | 0.814 | 0.278 | 0.472 | 0.955 | 0.446 | |
RSD | 0.133 | 10.9 | 0.255 | 354 | 194 | 68.2 | 379 | |
r2 | 0.647 | 0.547 | 0.945 | 0.430 | 0.382 | 0.598 | 0.775 | |
Adjusted r2 | 0.594 | 0.479 | 0.937 | 0.345 | 0.289 | 0.538 | 0.741 | |
Mean values for the main effects | ||||||||
Type | Non-smoked | 0.228 | 0.451 | nd | 6.59 | 64.3 | 31.3 | 126 |
Smoked | 0.550 | 21.5 | 1.93 | 525 | 337 | 181 | 1384 | |
Stage of filling | Beginning | 0.375 | 13.3 | 0.952 | 345 | 202 | 119 | 872 |
End | 0.402 | 8.65 | 0.975 | 187 | 199 | 93 | 638 |
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Valente, I.M.; Sousa, C.S.; Guido, L.F. Impact of the Smoking Process on Biogenic Amine Levels in Traditional Dry-Cured Chorizo. Separations 2023, 10, 585. https://doi.org/10.3390/separations10120585
Valente IM, Sousa CS, Guido LF. Impact of the Smoking Process on Biogenic Amine Levels in Traditional Dry-Cured Chorizo. Separations. 2023; 10(12):585. https://doi.org/10.3390/separations10120585
Chicago/Turabian StyleValente, Inês M., Cláudia S. Sousa, and Luís F. Guido. 2023. "Impact of the Smoking Process on Biogenic Amine Levels in Traditional Dry-Cured Chorizo" Separations 10, no. 12: 585. https://doi.org/10.3390/separations10120585