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Article

Compartment Model of Mixing in a Bubble Trap and Its Impact on Chromatographic Separations

1
Department of Biotechnology, University of Natural Resources and Life Sciences Vienna, Muthgasse 18, 1190 Vienna, Austria
2
Forschungszentrum Jülich, IBG-1: Biotechnology, 52428 Jülich, Germany
*
Author to whom correspondence should be addressed.
Processes 2020, 8(7), 780; https://doi.org/10.3390/pr8070780
Received: 8 June 2020 / Revised: 28 June 2020 / Accepted: 1 July 2020 / Published: 3 July 2020
(This article belongs to the Special Issue Redesign Processes in the Age of the Fourth Industrial Revolution)
Chromatography equipment includes hold-up volumes that are external to the packed bed and usually not considered in the development of chromatography models. These volumes can substantially contribute to band-broadening in the system and deteriorate the predicted performance. We selected a bubble trap of a pilot scale chromatography system as an example for a hold-up volume with a non-standard mixing behavior. In a worst-case scenario, the bubble trap is not properly flushed before elution, thus causing the significant band-broadening of the elution peak. We showed that the mixing of buffers with different densities in the bubble trap device can be accurately modeled using a simple compartment model. The model was calibrated at a wide range of flow rates and salt concentrations. The simulations were performed using the open-source software CADET, and all scripts and data are published with this manuscript. The results illustrate the importance of including external holdup volumes in chromatography modeling. The band-broadening effect of tubing, pumps, valves, detectors, frits, or any other zones with non-standard mixing behavior can be considered in very similar ways. View Full-Text
Keywords: preparative chromatography; mechanistic modelling; extra-column dispersion; peak broadening; fluid dynamics preparative chromatography; mechanistic modelling; extra-column dispersion; peak broadening; fluid dynamics
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MDPI and ACS Style

Beck, J.; Heymann, W.; von Lieres, E.; Hahn, R. Compartment Model of Mixing in a Bubble Trap and Its Impact on Chromatographic Separations. Processes 2020, 8, 780. https://doi.org/10.3390/pr8070780

AMA Style

Beck J, Heymann W, von Lieres E, Hahn R. Compartment Model of Mixing in a Bubble Trap and Its Impact on Chromatographic Separations. Processes. 2020; 8(7):780. https://doi.org/10.3390/pr8070780

Chicago/Turabian Style

Beck, Jürgen, William Heymann, Eric von Lieres, and Rainer Hahn. 2020. "Compartment Model of Mixing in a Bubble Trap and Its Impact on Chromatographic Separations" Processes 8, no. 7: 780. https://doi.org/10.3390/pr8070780

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