Next Article in Journal
On the Impacts of Pre-Heated Natural Gas Injection in Blast Furnaces
Next Article in Special Issue
Determination of the Total Polyphenols Content and Antioxidant Activity of Echinacea Purpurea Extracts Using Newly Manufactured Glassy Carbon Electrodes Modified with Carbon Nanotubes
Previous Article in Journal
Volatile Esters and Fusel Alcohol Concentrations in Beer Optimized by Modulation of Main Fermentation Parameters in an Industrial Plant
Previous Article in Special Issue
Industrial-Scale Study of the Chemical Composition of Olive Oil Process-Derived Matrices
Open AccessArticle

Moringa oleifera—Storage Stability, In Vitro-Simulated Digestion and Cytotoxicity Assessment of Microencapsulated Extract

1
School of Agronomy, Autonomous University of Nuevo Leon, Nuevo León 66050, Mexico
2
School of Chemistry, Autonomous University of Coahuila, Coahuila 25280, Mexico
3
Food Processing Group, International Iberian Nanotechnology Laboratory, Av. Mestre José Veiga S/N, 4715-330 Braga, Portugal
4
School of Health Sciences, Autonomous University of Coahuila, Coahuila 26090, Mexico
*
Author to whom correspondence should be addressed.
Processes 2020, 8(7), 770; https://doi.org/10.3390/pr8070770
Received: 1 June 2020 / Revised: 23 June 2020 / Accepted: 28 June 2020 / Published: 1 July 2020
Moringa extract was microencapsulated for the first time by spray-drying technique using tragacanth gum (MorTG) to improve its stability under gastrointestinal and storage conditions, assessing total polyphenolic content (TPC) and antioxidant activity. Additionally, cytotoxicity of the microencapsulated components was evaluated after contact with Caco-2 cells. Results showed that TPC was released as follows—oral (9.7%) < gastric (35.2%) < intestinal (57.6%). In addition, the antioxidant activity in in vitro digestion reached up to 16.76 ±0.15 mg GAE g−1, which was 300% higher than the initial value. Furthermore, microencapsulated moringa extract presented a half-life up to 45 days of storage, where the noticeably change was observed at 35 °C and 52.9% relative humidity. Finally, direct treatment with 0.125 mg mL−1 MorTG on Caco-2 cells showed a slight antiproliferative effect, with a cell viability of approx. 87%. Caco-2 cells’ viability demonstrated non-cytotoxicity, supporting the safety of the proposed formulation and potential use within the food field. View Full-Text
Keywords: Moringa oleifera; microencapsulation; cell viability; storage; in vitro digestion; polyphenols Moringa oleifera; microencapsulation; cell viability; storage; in vitro digestion; polyphenols
Show Figures

Graphical abstract

MDPI and ACS Style

Castro-López, C.; Gonçalves, C.; Ventura-Sobrevilla, J.M.; Pastrana, L.M.; Aguilar-González, C.N.; Martínez-Ávila, G.C.G. Moringa oleifera—Storage Stability, In Vitro-Simulated Digestion and Cytotoxicity Assessment of Microencapsulated Extract. Processes 2020, 8, 770.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop