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Article

Novel Purification Process for Amyloid Beta Peptide(1-40)

Faculty of Frontiers of Innovative Research in Science and Technology (FIRST), Konan University, Chuo-ku, Kobe 650-0047, Japan
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Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Processes 2020, 8(4), 464; https://doi.org/10.3390/pr8040464
Received: 12 March 2020 / Revised: 27 March 2020 / Accepted: 8 April 2020 / Published: 15 April 2020
(This article belongs to the Special Issue Advances of Peptide Engineering)
Amyloid beta peptide (Aβ)-related studies require an adequate supply of purified Aβ peptide. However, Aβ peptides are “difficult sequences” to synthesize chemically, and low yields are common due to aggregation during purification. Here, we demonstrate an easier synthesis, deprotection, reduction, cleavage, and purification process for Aβ(1-40) using standard 9-fluorenylmethyloxycarbonyl (Fmoc)-protected amino acids and solid-phase peptide synthesis (SPPS) resin [HMBA (4-hydroxymethyl benzamide) resin] that provides higher yields of Aβ(1-40) than previous standard protocols. Furthermore, purification requires a similar amount of time as conventional purification processes, although the peptide must be cleaved from the resin immediately prior to purification. The method described herein is not limited to the production of Aβ(1-40), and can be used to synthesize other easily-oxidized and aggregating sequences. Our proposed methodology will contribute to various fields using “difficult sequence” peptides, such as pharmaceutical and materials science, as well as research for the diagnosis and treatment of protein/peptide misfolding diseases. View Full-Text
Keywords: peptide synthesis; reduction; purification; amyloid beta peptide; solid-phase synthesis; difficult sequence; aggregating peptide peptide synthesis; reduction; purification; amyloid beta peptide; solid-phase synthesis; difficult sequence; aggregating peptide
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MDPI and ACS Style

Usui, K.; Yokota, S.-i.; Iwata, K.; Hamada, Y. Novel Purification Process for Amyloid Beta Peptide(1-40). Processes 2020, 8, 464. https://doi.org/10.3390/pr8040464

AMA Style

Usui K, Yokota S-i, Iwata K, Hamada Y. Novel Purification Process for Amyloid Beta Peptide(1-40). Processes. 2020; 8(4):464. https://doi.org/10.3390/pr8040464

Chicago/Turabian Style

Usui, Kenji, Shin-ichiro Yokota, Kazuya Iwata, and Yoshio Hamada. 2020. "Novel Purification Process for Amyloid Beta Peptide(1-40)" Processes 8, no. 4: 464. https://doi.org/10.3390/pr8040464

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