Synthesis of Dietetic Structured Lipids from Spent Coffee Grounds Crude Oil Catalyzed by Commercial Immobilized Lipases and Immobilized Rhizopus oryzae Lipase on Biochar and Hybrid Support
, Jefferson C. B. Santos 2,3, Diana Faria 1, Álvaro S. Lima 2,3, Laiza C. Krause 2,3, Cleide M. F. Soares 2,3 and Suzana Ferreira-Dias 1,*Round 1
Reviewer 1 Report
It is a very well-written and well-structured manuscript. a few minor comments:
Table 3: Please add the abbreviation of silverskin oils to the caption, too.
Figure 1 and 2: for better comparison, authors could merge these 2 figures in 1.
Author Response
ANSWERS TO THE REVIEWERS COMMENTS/SUGGESTIONS
First of all, we would like to thank you for the time and valuable suggestions which greatly helped to improve our manuscript entitled “Synthesis of dietetic structured lipids from spent coffee grounds crude oil catalyzed by commercial immobilized lipases and immobilized Rhizopus oryzae lipase on biochar and hybrid support”, submitted to be considered for publication in Journal Processes as a research article.
All the modifications in the original manuscript are highlighted in yellow.
We hope all the questions were answered accordingly and this version of the manuscript will meet the required standards for publication in Processes.
Suzana Ferreira-Dias
REVIEWER 1
(1) Reviewer 1: Table 3: Please add the abbreviation of silverskin oils to the caption, too.
Answer: The information was added to the caption of Table 3.
(2) Reviewer 1: Figure 1 and 2: for better comparison, authors could merge these 2 figures in 1.
Answer: As suggested, both figures were merged.
Reviewer 2 Report
This manuscript describes a comprehensive study of the valorization of coffee industry residues for production of structured lipids (SL). The circular economy approach is particularly interesting, since the authors propose using the selected residues as reaction substrates and biocatalysts immobilization supports.
A considerable amount of work is presented, including residues characterization, enzymatic reactions, biocatalyst immobilization and stability. Also, different enzymes and immobilization methods are compared.
The manuscript is well written, the methods are clearly explained, and the discussion is sound and supported by suitable bibliographic sources. There is scarce information on SL enzymatic production, thus there is also novelty added-value in this work.
Therefore, I recommend publication of the manuscript, provided that some minor aspects are corrected:
- English language needs some editing (i.e. "immobilization on" should be used instead of "immobilization in")
- Pay attention to superscripts when writing units
- When comparing enzyme activities/stabilities, I suggest using percentages to ease comparison (i.e. data on Table 4)
- Figure captions should include all the necessary information on what is shown in the figure (please revise captions for Figures 1 and 2)
- Demonstrate graphically the fitting of the enzyme deactivation models described on Table 5
Author Response
ANSWERS TO THE REVIEWERS COMMENTS/SUGGESTIONS
First of all, we would like to thank you for the time and valuable suggestions which greatly helped to improve our manuscript entitled “Synthesis of dietetic structured lipids from spent coffee grounds crude oil catalyzed by commercial immobilized lipases and immobilized Rhizopus oryzae lipase on biochar and hybrid support”, submitted to be considered for publication in Journal Processes as a research article.
All the modifications in the original manuscript are highlighted in yellow.
We hope all the questions were answered accordingly and this version of the manuscript will meet the required standards for publication in Processes.
Sincerely yours,
Suzana Ferreira-Dias
(1) Reviewer 2: English language needs some editing (i.e. "immobilization on" should be used instead of "immobilization in").
Answer: The required English changes were performed in the manuscript and all the manuscript was revised.
(2) Reviewer 2: Pay attention to superscripts when writing units.
Answer: We acknowledge the recommendation. The text was corrected accordingly.
(3) Reviewer 2: When comparing enzyme activities/stabilities, I suggest using percentages to ease comparison (i.e. data on Table 4).
Answer: In Table 4, activity data were converted to percentage values. Therefore, the text was modified accordingly. However, we decided to keep the absolute activity values in the text. Thank you for your suggestion.
(4) Reviewer 2: Figure captions should include all the necessary information on what is shown in the figure (please revise captions for Figures 1 and 2).
Answer: Figures 1 and 2 were merged as suggested by reviewer 1 and the caption was modified and complemented with missing information.
(5) Reviewer 2: Demonstrate graphically the fitting of the enzyme deactivation models described on Table 5.
Answer: The fitting of the enzyme deactivation models described on Table 5 are shown in the Figure 4 and corresponds to the dotted lines. Now, in the legends of Fig. 4 (a) and (b), the dotted lines are identified as “first-order model” and “sigmoid order”, respectively. Moreover, the identification of each deactivation model was added to Figure caption for better understanding.
