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Open AccessArticle

The Effect of Extraction Methods on Preliminary Structural Properties and Antioxidant Activities of Polysaccharides from Lactarius vividus

1
College of Life Sciences, Sichuan Agricultural University, Yaan 625014, China
2
College of Agricultural Science, Xichang University, Xichang 615000, China
*
Author to whom correspondence should be addressed.
These authors contributed equally to this paper.
Processes 2019, 7(8), 482; https://doi.org/10.3390/pr7080482
Received: 30 June 2019 / Revised: 24 July 2019 / Accepted: 25 July 2019 / Published: 30 July 2019
(This article belongs to the Special Issue Green Separation and Extraction Processes)
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Abstract

Four polysaccharides (LVP-u, LVP-m, LVP-e, and LVP-h) were extracted from the fruiting bodies of Lactarius vividus by an ultrasonic-assisted extraction method, microwave-assisted extraction method, enzyme-assisted extraction method, and hot water extraction method, respectively. Then, the effect of extraction methods on yields, preliminary structural properties, and antioxidant activities was systematically investigated using the weighing method, chemical composition analysis, high-performance gel permeation chromatography (HPGPC), high-performance liquid chromatography (HPLC), Fourier-transform infrared spectroscopy (FT-IR), scanning electron microscope (SEM), radical scavenging, and metal ion chelating assays. Results demonstrated that the four L. vividus polysaccharides (LVPs) were all combined with protein, and the yield of LVP-u was higher than others. Molecular weight distribution, monosaccharide and amino acid compositions, and microstructures among the four LVPs were significantly different. Moreover, the LVPs showed significant antioxidant activities in a dose-dependent manner, and LVP-e demonstrated better antioxidant activities in superoxide anion radical scavenging activity assays and metal ion chelating activity assays, while LVP-u showed higher activity in its hydroxyl radical scavenging ability. View Full-Text
Keywords: Lactarius vividus; polysaccharides; extraction methods; preliminary structure properties; antioxidant activities Lactarius vividus; polysaccharides; extraction methods; preliminary structure properties; antioxidant activities
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Xu, Z.; Feng, S.; Qu, J.; Yuan, M.; Yang, R.; Zhou, L.; Chen, T.; Ding, C. The Effect of Extraction Methods on Preliminary Structural Properties and Antioxidant Activities of Polysaccharides from Lactarius vividus. Processes 2019, 7, 482.

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