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Sustainable Green Procedure for Extraction of Hesperidin from Selected Croatian Mandarin Peels

Faculty of Food Technology Osijek, Josip Juraj Strossmayer University of Osijek, Franje Kuhača 20, 31000 Osijek, Croatia
Faculty of Agronomy and Food Technology, University of Mostar, Biskupa Čule bb, 88000 Mostar, Bosnia and Herzegovina
Author to whom correspondence should be addressed.
Processes 2019, 7(7), 469;
Received: 27 June 2019 / Revised: 17 July 2019 / Accepted: 18 July 2019 / Published: 20 July 2019
(This article belongs to the Special Issue Green Separation and Extraction Processes)
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The peels of Citrus reticulata Blanco mandarin cultivars of different Croatian varieties (Zorica rana, Chahara, Okitsu, Kuno) were extracted using 15 different choline chloride-based deep eutectic solvents (DESs) at 50 °C for 30 min and with 20% water addition. The extracts were analyzed by high performance liquid chromatography with diode array detection (HPLC-DAD) to determine the most suitable DES for the extraction of hesperidin in the samples. The screening results indicated that choline chloride: acetamide (1:2) provided the most efficient hesperidin extraction (112.14 mg/g of plant), while choline chloride:citric acid (1:1) solvent showed the lowest hesperidin yield (1.44 mg/g of plant). The Box–Behnken design was employed to optimize extraction parameters for each variety of mandarin peel, including extraction time, temperature and water content on hesperidin extraction. The results indicated that hesperidin content in mandarin peels was completely variety-dependent. Being a novel and efficient green media for hesperidin extraction, deep eutectic solvents could also serve as promising solvent systems for the production of extracts rich in bioactive compounds. View Full-Text
Keywords: by-product; deep eutectic solvents; hesperidin; mandarin peel; optimization by-product; deep eutectic solvents; hesperidin; mandarin peel; optimization

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Jokić, S.; Šafranko, S.; Jakovljević, M.; Cikoš, A.-M.; Kajić, N.; Kolarević, F.; Babić, J.; Molnar, M. Sustainable Green Procedure for Extraction of Hesperidin from Selected Croatian Mandarin Peels. Processes 2019, 7, 469.

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