Preparation and Characterization of Furosemide-Silver Complex Loaded Chitosan Nanoparticles
AbstractAntibiotic-resistant bacteria may result in serious infections which are difficult to treat. In addition, the poor antibiotic pipeline has contributed to the crisis. Recently, a complex of furosemide and silver (Ag-FSE) has been reported as a potential antibacterial agent. However, its poor aqueous solubility is limiting its activity. The purpose of this study was to encapsulate Ag-FSE into chitosan nanoparticles (CSNPs) and evaluate antibacterial efficacy. Ag-FSE CSNPs were prepared using an ionic gelation technique. The particle size, polydispersity index, and zeta potential of Ag-FSE CSNPs were 197.1 ± 3.88 nm 0.234 ± 0.018 and 36.7 ± 1.78 mV, respectively. Encapsulation efficiency was 66.72 ± 4.14%. In vitro antibacterial activity results showed that there was 3- and 6-fold enhanced activity with Ag-FSE CSNPs against E. coli and S. aureus, respectively. Results also confirmed that Ag-FSE CSNPs showed ~44% release within 4 h at pH 5.5 and 6.5. Moreover, release from the CSNPs was sustained with a cumulative release of ~75% over a period of 24 h. In conclusion, encapsulation of Ag-FSE into CSNPs resulted in significant improvement of antibacterial efficacy with a sustained and pH-sensitive release. Therefore, Ag-FSE CSNPs can be considered as a potential novel antibacterial agent against bacterial infections. View Full-Text
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Rodriguez, V.A.; Bolla, P.K.; Kalhapure, R.S.; Boddu, S.H.S.; Neupane, R.; Franco, J.; Renukuntla, J. Preparation and Characterization of Furosemide-Silver Complex Loaded Chitosan Nanoparticles. Processes 2019, 7, 206.
Rodriguez VA, Bolla PK, Kalhapure RS, Boddu SHS, Neupane R, Franco J, Renukuntla J. Preparation and Characterization of Furosemide-Silver Complex Loaded Chitosan Nanoparticles. Processes. 2019; 7(4):206.Chicago/Turabian Style
Rodriguez, Victor A.; Bolla, Pradeep K.; Kalhapure, Rahul S.; Boddu, Sai H.S.; Neupane, Rabin; Franco, Julian; Renukuntla, Jwala. 2019. "Preparation and Characterization of Furosemide-Silver Complex Loaded Chitosan Nanoparticles." Processes 7, no. 4: 206.