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Processes 2019, 7(4), 206; https://doi.org/10.3390/pr7040206

Preparation and Characterization of Furosemide-Silver Complex Loaded Chitosan Nanoparticles

1
School of Pharmacy, University of Texas at El Paso, 1101 N. Campbell St, El Paso, TX 79902, USA
2
Department of Biomedical Engineering, College of Engineering, University of Texas at El Paso, 500 W University Ave, El Paso, TX 79968, USA
3
Department of Pharmacy Practice, The University of Toledo HSC, College of Pharmacy and Pharmaceutical Sciences, 3000 Arlington Ave. (MS1013) Toledo, OH 43614, USA
*
Author to whom correspondence should be addressed.
Received: 13 February 2019 / Revised: 8 March 2019 / Accepted: 3 April 2019 / Published: 11 April 2019
(This article belongs to the Special Issue Protein Biosynthesis and Drug Design & Delivery Processes)
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Abstract

Antibiotic-resistant bacteria may result in serious infections which are difficult to treat. In addition, the poor antibiotic pipeline has contributed to the crisis. Recently, a complex of furosemide and silver (Ag-FSE) has been reported as a potential antibacterial agent. However, its poor aqueous solubility is limiting its activity. The purpose of this study was to encapsulate Ag-FSE into chitosan nanoparticles (CSNPs) and evaluate antibacterial efficacy. Ag-FSE CSNPs were prepared using an ionic gelation technique. The particle size, polydispersity index, and zeta potential of Ag-FSE CSNPs were 197.1 ± 3.88 nm 0.234 ± 0.018 and 36.7 ± 1.78 mV, respectively. Encapsulation efficiency was 66.72 ± 4.14%. In vitro antibacterial activity results showed that there was 3- and 6-fold enhanced activity with Ag-FSE CSNPs against E. coli and S. aureus, respectively. Results also confirmed that Ag-FSE CSNPs showed ~44% release within 4 h at pH 5.5 and 6.5. Moreover, release from the CSNPs was sustained with a cumulative release of ~75% over a period of 24 h. In conclusion, encapsulation of Ag-FSE into CSNPs resulted in significant improvement of antibacterial efficacy with a sustained and pH-sensitive release. Therefore, Ag-FSE CSNPs can be considered as a potential novel antibacterial agent against bacterial infections. View Full-Text
Keywords: Chitosan nanoparticles; ionic-gelation; antibiotic resistance; furosemide-silver complex; antibacterial; pH-sensitive; staphylococcus aureus Chitosan nanoparticles; ionic-gelation; antibiotic resistance; furosemide-silver complex; antibacterial; pH-sensitive; staphylococcus aureus
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Rodriguez, V.A.; Bolla, P.K.; Kalhapure, R.S.; Boddu, S.H.S.; Neupane, R.; Franco, J.; Renukuntla, J. Preparation and Characterization of Furosemide-Silver Complex Loaded Chitosan Nanoparticles. Processes 2019, 7, 206.

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