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TLC-Direct Bioautography as a High Throughput Method for Detection of Antimicrobials in Plants

Department of Chromatographic Methods, Maria Curie-Skłodowska University, Maria Curie-Skłodowska Sq. 3, 20-031 Lublin, Poland
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Academic Editor: Mark Devlin Maloney
Chromatography 2015, 2(2), 225-238; https://doi.org/10.3390/chromatography2020225
Received: 3 March 2015 / Accepted: 12 May 2015 / Published: 18 May 2015
(This article belongs to the Special Issue New Trends in Thin-Layer Chromatography)
The richness of bioactive compounds in plant materials encourages continuous development of separation methods and bioassays for their isolation and identification. Thin-layer chromatography-direct bioautography links separation on the adsorbent layer with biological tests performed directly on it. Therefore, the method is very convenient for searching plant constituents with biological activity, such as antibiotics. Test bacteria grow directly on a plate surface excluding places where antibacterials are located. They can be detected with reagents converted by living bacteria. TLC-DB is a high throughput method enabling analyses of many samples in parallel and the comparison of their activity. Both screening and semi-quantitative analysis is possible. The targeted compounds can be identified using spectroscopic methods, mostly mass spectrometry, that can be performed directly on a TLC plate. This paper discusses all above mentioned aspects of TLC-DB, illustrating them with literature, schemes and our own results. View Full-Text
Keywords: thin-layer chromatography-direct bioautography; effect-directed analysis, antimicrobials; biological screening thin-layer chromatography-direct bioautography; effect-directed analysis, antimicrobials; biological screening
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Choma, I.M.; Jesionek, W. TLC-Direct Bioautography as a High Throughput Method for Detection of Antimicrobials in Plants. Chromatography 2015, 2, 225-238.

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