Epigenome-Wide Analysis Reveals DNA Methylation Alteration in ZFP57 and Its Target RASGFR2 in a Mexican Population Cohort with Autism
Abstract
:1. Introduction
2. Materials and Methods
2.1. Subjects and Samples
2.2. DNA Extraction and Bisulfite Conversion
2.3. Ancestry Analysis
2.4. Genome-Wide Analysis of Differential DNA Methylation
3. Results
3.1. Clinical and Demographic Data
3.2. Differentially Methylated Regions between ASD and TD Individuals
3.3. Differentially Methylated Regions Affected by Sex and ASD Diagnosis
4. Discussion
4.1. Differentially Methylated Regions between ASD and TD Children Relate to Genes Involved in Neurodevelopment
4.2. Differentially Methylated Regions in ASD Interact with Sex
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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ASD | TD | p-Value | |
---|---|---|---|
Subject Age (mean, SD) | 5.2, 1.9 | 5.6, 1.09 | 0.7645 1 |
(range) | 3–12 | 4–7 | |
Gender | |||
Male:Female | 22:5 | 6:9 | 0.01479 1 |
Gene a | Chromosome | Width (Base Pairs) | False Discovery Rate Corrected p-Value | Methylation Differences (ASD-TD) b |
---|---|---|---|---|
FAIM, Fas Apoptotic Inhibitory Molecule 2 | chr12 | 469 | 3.18 × 10−3 | −0.087 |
CPXM2, Carboxypeptidase X, M14 Family Member 2 | chr10 | 674 | 4.20 × 10−3 | 0.056 |
NRIP2, Nuclear Receptor Interacting Protein 2 | chr12 | 592 | 8.99 × 10−3 | 0.052 |
SOX7, CTD-2135J3.3 SRY-Box7 | chr8 | 205 | 3.03 × 10−2 | −0.061 |
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Aspra, Q.; Cabrera-Mendoza, B.; Morales-Marín, M.E.; Márquez, C.; Chicalote, C.; Ballesteros, A.; Aguilar, M.; Castro, X.; Gómez-Cotero, A.; Balboa-Verduzco, A.M.; et al. Epigenome-Wide Analysis Reveals DNA Methylation Alteration in ZFP57 and Its Target RASGFR2 in a Mexican Population Cohort with Autism. Children 2022, 9, 462. https://doi.org/10.3390/children9040462
Aspra Q, Cabrera-Mendoza B, Morales-Marín ME, Márquez C, Chicalote C, Ballesteros A, Aguilar M, Castro X, Gómez-Cotero A, Balboa-Verduzco AM, et al. Epigenome-Wide Analysis Reveals DNA Methylation Alteration in ZFP57 and Its Target RASGFR2 in a Mexican Population Cohort with Autism. Children. 2022; 9(4):462. https://doi.org/10.3390/children9040462
Chicago/Turabian StyleAspra, Queletzu, Brenda Cabrera-Mendoza, Mirna Edith Morales-Marín, Carla Márquez, Carlos Chicalote, Ana Ballesteros, Miriam Aguilar, Xochitl Castro, Amalia Gómez-Cotero, Ana María Balboa-Verduzco, and et al. 2022. "Epigenome-Wide Analysis Reveals DNA Methylation Alteration in ZFP57 and Its Target RASGFR2 in a Mexican Population Cohort with Autism" Children 9, no. 4: 462. https://doi.org/10.3390/children9040462