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Biomedicines
Volume 9
Issue 11
10.3390/biomedicines9111692
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Article

Peri-Prostatic Adipocyte-Released TGFβ Enhances Prostate Cancer Cell Motility by Upregulation of Connective Tissue Growth Factor

by
Evelina La Civita
1,
Antonietta Liotti
1,
Michele Cennamo
1,
Felice Crocetto
2,
Matteo Ferro
3,
Pasquale Liguoro
1,
Amelia Cimmino
4,
Ciro Imbimbo
2,
Francesco Beguinot
1,
Pietro Formisano
1,* and
Daniela Terracciano
1,*
1
Department of Translational Medical Sciences, University of Naples “Federico II”, 80131 Naples, Italy
2
Department of Neurosciences, Sciences of Reproduction and Odontostomatology, University of Naples “Federico II”, 80131 Naples, Italy
3
Division of Urology, European Institute of Oncology, 20141 Milan, Italy
4
Institute of Genetics and Biophysic, National Research Council, 80131 Naples, Italy
*
Authors to whom correspondence should be addressed.
Biomedicines 2021, 9(11), 1692; https://doi.org/10.3390/biomedicines9111692
Submission received: 28 October 2021 / Revised: 9 November 2021 / Accepted: 12 November 2021 / Published: 15 November 2021
(This article belongs to the Special Issue Obesity and Diabetes: Impact on Cancer)
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Abstract

Periprostatic adipose tissue (PPAT) has emerged as a key player in the prostate cancer (PCa) microenvironment. In this study, we evaluated the ability of PPAT to promote PCa cell migration, as well as the molecular mechanisms involved. Methods: We collected conditioned mediums from in vitro differentiated adipocytes isolated from PPAT taken from PCa patients during radical prostatectomy. Migration was studied by scratch assay. Results: Culture with CM of human PPAT (AdipoCM) promotes migration in two different human androgen-independent (AI) PCa cell lines (DU145 and PC3) and upregulated the expression of CTGF. SB431542, a well-known TGFβ receptor inhibitor, counteracts the increased migration observed in presence of AdipoCM and decreased CTGF expression, suggesting that a paracrine secretion of TGFβ by PPAT affects motility of PCa cells. Conclusions: Collectively, our study showed that factors secreted by PPAT enhanced migration through CTGF upregulation in AI PCa cell lines. These findings reveal the potential of novel therapeutic strategies targeting adipocyte-released factors and TGFβ/CTGF axis to fight advanced PCa dissemination.
Keywords: adipocytes; prostate cancer; TGFβ1; peri-prostatic adipose tissue; cell migration adipocytes; prostate cancer; TGFβ1; peri-prostatic adipose tissue; cell migration

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MDPI and ACS Style

La Civita, E.; Liotti, A.; Cennamo, M.; Crocetto, F.; Ferro, M.; Liguoro, P.; Cimmino, A.; Imbimbo, C.; Beguinot, F.; Formisano, P.; et al. Peri-Prostatic Adipocyte-Released TGFβ Enhances Prostate Cancer Cell Motility by Upregulation of Connective Tissue Growth Factor. Biomedicines 2021, 9, 1692. https://doi.org/10.3390/biomedicines9111692

AMA Style

La Civita E, Liotti A, Cennamo M, Crocetto F, Ferro M, Liguoro P, Cimmino A, Imbimbo C, Beguinot F, Formisano P, et al. Peri-Prostatic Adipocyte-Released TGFβ Enhances Prostate Cancer Cell Motility by Upregulation of Connective Tissue Growth Factor. Biomedicines. 2021; 9(11):1692. https://doi.org/10.3390/biomedicines9111692

Chicago/Turabian Style

La Civita, Evelina, Antonietta Liotti, Michele Cennamo, Felice Crocetto, Matteo Ferro, Pasquale Liguoro, Amelia Cimmino, Ciro Imbimbo, Francesco Beguinot, Pietro Formisano, and et al. 2021. "Peri-Prostatic Adipocyte-Released TGFβ Enhances Prostate Cancer Cell Motility by Upregulation of Connective Tissue Growth Factor" Biomedicines 9, no. 11: 1692. https://doi.org/10.3390/biomedicines9111692

APA Style

La Civita, E., Liotti, A., Cennamo, M., Crocetto, F., Ferro, M., Liguoro, P., Cimmino, A., Imbimbo, C., Beguinot, F., Formisano, P., & Terracciano, D. (2021). Peri-Prostatic Adipocyte-Released TGFβ Enhances Prostate Cancer Cell Motility by Upregulation of Connective Tissue Growth Factor. Biomedicines, 9(11), 1692. https://doi.org/10.3390/biomedicines9111692

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