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Biomedicines
Volume 8
Issue 12
10.3390/biomedicines8120609
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Article

Combinatorial Effect of Magnetic Field and Radiotherapy in PDAC Organoids: A Pilot Study

by
Luca Nicosia
1,†,
Filippo Alongi
1,2,†,
Silvia Andreani
3,
Ruggero Ruggieri
1,
Borislav Rusev
3,
Beatrice Mantoan
1,
Rita Teresa Lawlor
3,
Antonio Pea
4,
Aldo Scarpa
3,5,
Linda Agolli
6,7,
Vincenzo Corbo
3,5,*,‡ and
Sabrina D’Agosto
5,‡
1
Advanced Radiation Oncology Department, IRCCS Sacro Cuore Don Calabria Hospital, Cancer Care Center, 37024 Negrar, Italy
2
Medicine Faculty, University of Brescia, 25121 Brescia, Italy
3
ARC-Net Research Centre, University and Hospital Trust of Verona, 37134 Verona, Italy
4
General and Pancreatic Surgery Department, Pancreas Institute, University and Hospital Trust of Verona, 37134 Verona, Italy
5
Department of Diagnostics and Public Health, University and Hospital Trust of Verona, 37134 Verona, Italy
6
Department of Radiation Oncology, Faculty of Medicine and University Hospital Carl Gustav Carus, Technische Universität, Fetscherstraße 74, 01307 Dresden, Germany
7
OncoRay—National Center for Radiation Research in Oncology, Faculty of Medicine and University Hospital Carl Gustav Carus, Technische Universität Dresden, Fetscherstraße 74, 01307 Dresden, Germany
*
Author to whom correspondence should be addressed.
†
Share first co-authorship.
‡
Share last co-authorship.
Biomedicines 2020, 8(12), 609; https://doi.org/10.3390/biomedicines8120609
Submission received: 10 November 2020 / Revised: 6 December 2020 / Accepted: 11 December 2020 / Published: 14 December 2020
(This article belongs to the Special Issue New Insights in Radiotherapy)
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Abstract

Pancreatic ductal adenocarcinoma (PDAC) is highly refractory to systemic treatment, including radiotherapy (RT) either as alone or in combination with chemotherapy. Magnetic resonance (MR)-guided RT is a novel treatment technique which conjugates the high MR imaging contrast resolution to the possibility of re-adapting treatment plan to daily anatomical variations. Magnetic field (MF) might exert a biological effect that could be exploited to enhance radiation effect. The aim of the present study was to lay the preclinical basis of the MF effect by exploring how it modifies the response to radiation in organoid cultures established from PDAC. The short-term effect of radiation, alone or in combination with MF, was evaluated in patient-derived organoids (PDOs) and monolayer cell cultures. Cell viability, apoptotic cell death, and organoid size following exposure to the treatment were evaluated. PDOs demonstrated limited sensitivity at clinically relevant doses of radiation. The combination of radiation and MF demonstrated superior efficacy than monotherapy in almost all the PDOs tested. PDOs treated with combination of radiation and MF were significantly smaller in size and some showed increased cell death as compared to the monotherapy with radiation. Long-time exposure to 1.5T MF can increase the therapeutic efficacy of radiation in PDAC organoids.
Keywords: patient-derived models; 3D organoids; MR-guided radiotherapy; MR-Linac; radiotherapy; magnetic field patient-derived models; 3D organoids; MR-guided radiotherapy; MR-Linac; radiotherapy; magnetic field

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MDPI and ACS Style

Nicosia, L.; Alongi, F.; Andreani, S.; Ruggieri, R.; Rusev, B.; Mantoan, B.; Lawlor, R.T.; Pea, A.; Scarpa, A.; Agolli, L.; et al. Combinatorial Effect of Magnetic Field and Radiotherapy in PDAC Organoids: A Pilot Study. Biomedicines 2020, 8, 609. https://doi.org/10.3390/biomedicines8120609

AMA Style

Nicosia L, Alongi F, Andreani S, Ruggieri R, Rusev B, Mantoan B, Lawlor RT, Pea A, Scarpa A, Agolli L, et al. Combinatorial Effect of Magnetic Field and Radiotherapy in PDAC Organoids: A Pilot Study. Biomedicines. 2020; 8(12):609. https://doi.org/10.3390/biomedicines8120609

Chicago/Turabian Style

Nicosia, Luca, Filippo Alongi, Silvia Andreani, Ruggero Ruggieri, Borislav Rusev, Beatrice Mantoan, Rita Teresa Lawlor, Antonio Pea, Aldo Scarpa, Linda Agolli, and et al. 2020. "Combinatorial Effect of Magnetic Field and Radiotherapy in PDAC Organoids: A Pilot Study" Biomedicines 8, no. 12: 609. https://doi.org/10.3390/biomedicines8120609

APA Style

Nicosia, L., Alongi, F., Andreani, S., Ruggieri, R., Rusev, B., Mantoan, B., Lawlor, R. T., Pea, A., Scarpa, A., Agolli, L., Corbo, V., & D’Agosto, S. (2020). Combinatorial Effect of Magnetic Field and Radiotherapy in PDAC Organoids: A Pilot Study. Biomedicines, 8(12), 609. https://doi.org/10.3390/biomedicines8120609

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