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Biomedicines
Volume 11
Issue 3
10.3390/biomedicines11030987
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Article

Adipose-Derived Stromal Cells within a Gelatin Matrix Acquire Enhanced Regenerative and Angiogenic Properties: A Pre-Clinical Study for Application to Chronic Wounds

by
Nicolo Costantino Brembilla
1,2,
Ali Modarressi
3,
Dominik André-Lévigne
3,
Estelle Brioudes
4,
Florian Lanza
1,
Hubert Vuagnat
5,
Stéphane Durual
6,
Laurine Marger
6,
Wolf-Henning Boehncke
1,2,
Karl-Heinz Krause
1,4 and
Olivier Preynat-Seauve
4,7,*
1
Department of Pathology and Immunology, Faculty of Medicine, University of Geneva, 1205 Geneva, Switzerland
2
Division of Dermatology and Venereology, Geneva University Hospitals, 1205 Geneva, Switzerland
3
Division of Plastic, Reconstructive and Aesthetic Surgery, Geneva University Hospitals, 1205 Geneva, Switzerland
4
Laboratory of Therapy and Stem Cells, Geneva University Hospitals, 1205 Geneva, Switzerland
5
Program for Wounds and Wound Healing, Geneva University Hospitals, 1205 Geneva, Switzerland
6
Laboratory of Biomaterials, Faculty of Dental Medicine, University of Geneva, 1205 Geneva, Switzerland
7
Department of Medicine, Faculty of Medicine, University of Geneva, 1205 Geneva, Switzerland
*
Author to whom correspondence should be addressed.
Biomedicines 2023, 11(3), 987; https://doi.org/10.3390/biomedicines11030987
Submission received: 24 February 2023 / Revised: 13 March 2023 / Accepted: 15 March 2023 / Published: 22 March 2023
(This article belongs to the Special Issue Biomedical Potential of Stem Cells in Skin Regeneration and Wound Healing)
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Abstract

This study evaluates the influence of a gelatin sponge on adipose-derived stromal cells (ASC). Transcriptomic data revealed that, compared to ASC in a monolayer, a cross-linked porcine gelatin sponge strongly influences the transcriptome of ASC. Wound healing genes were massively regulated, notably with the inflammatory and angiogenic factors. Proteomics on conditioned media showed that gelatin also acted as a concentrator and reservoir of the regenerative ASC secretome. This secretome promoted fibroblast survival and epithelialization, and significantly increased the migration and tubular assembly of endothelial cells within fibronectin. ASC in gelatin on a chick chorioallantoic membrane were more connected to vessels than an empty sponge, confirming an increased angiogenesis in vivo. No tumor formation was observed in immunodeficient nude mice to which an ASC gelatin sponge was transplanted subcutaneously. Finally, ASC in a gelatin sponge prepared from outbred rats accelerated closure and re-vascularization of ischemic wounds in the footpads of rats. In conclusion, we provide here preclinical evidence that a cross-linked porcine gelatin sponge is an optimal carrier to concentrate and increase the regenerative activity of ASC, notably angiogenic. This formulation of ASC represents an optimal, convenient and clinically compliant option for the delivery of ASC on ischemic wounds.
Keywords: adipose stem cells; clinical translation; mesenchymal stem cells; stromal cells adipose stem cells; clinical translation; mesenchymal stem cells; stromal cells
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MDPI and ACS Style

Brembilla, N.C.; Modarressi, A.; André-Lévigne, D.; Brioudes, E.; Lanza, F.; Vuagnat, H.; Durual, S.; Marger, L.; Boehncke, W.-H.; Krause, K.-H.; et al. Adipose-Derived Stromal Cells within a Gelatin Matrix Acquire Enhanced Regenerative and Angiogenic Properties: A Pre-Clinical Study for Application to Chronic Wounds. Biomedicines 2023, 11, 987. https://doi.org/10.3390/biomedicines11030987

AMA Style

Brembilla NC, Modarressi A, André-Lévigne D, Brioudes E, Lanza F, Vuagnat H, Durual S, Marger L, Boehncke W-H, Krause K-H, et al. Adipose-Derived Stromal Cells within a Gelatin Matrix Acquire Enhanced Regenerative and Angiogenic Properties: A Pre-Clinical Study for Application to Chronic Wounds. Biomedicines. 2023; 11(3):987. https://doi.org/10.3390/biomedicines11030987

Chicago/Turabian Style

Brembilla, Nicolo Costantino, Ali Modarressi, Dominik André-Lévigne, Estelle Brioudes, Florian Lanza, Hubert Vuagnat, Stéphane Durual, Laurine Marger, Wolf-Henning Boehncke, Karl-Heinz Krause, and et al. 2023. "Adipose-Derived Stromal Cells within a Gelatin Matrix Acquire Enhanced Regenerative and Angiogenic Properties: A Pre-Clinical Study for Application to Chronic Wounds" Biomedicines 11, no. 3: 987. https://doi.org/10.3390/biomedicines11030987

APA Style

Brembilla, N. C., Modarressi, A., André-Lévigne, D., Brioudes, E., Lanza, F., Vuagnat, H., Durual, S., Marger, L., Boehncke, W.-H., Krause, K.-H., & Preynat-Seauve, O. (2023). Adipose-Derived Stromal Cells within a Gelatin Matrix Acquire Enhanced Regenerative and Angiogenic Properties: A Pre-Clinical Study for Application to Chronic Wounds. Biomedicines, 11(3), 987. https://doi.org/10.3390/biomedicines11030987

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