SDF-1α-Releasing Microspheres Effectively Extend Stem Cell Homing after Myocardial Infarction
Abstract
:1. Introduction
2. Methods
2.1. Animal Studies
2.2. Animal Experimental Design
2.3. Microspheres with SDF-1α
2.4. Enzyme-Linked Immunosorbent Assay (ELISA)
2.5. RNA Isolation and Quantitative RT-PCR
2.6. Statistical Analysis
3. Results
3.1. Intrapericardial Administration of Microspheres Increases the Concentration of SDF-1α in the Myocardium
3.2. SDF-1α-Releasing Microspheres Affect Gene Expression in the Heart after MI
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Control Group | Study Group | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
Animals number | n = 3 | n = 3 | n = 3 | n = 3 | n = 3 | n = 3 | n = 3 | n = 3 | n = 3 | n = 3 | n = 3 |
Days after MI | 24 h | 72 h | 7 d | 14 d | 15 d | 17 d | 21 d | 28 d | 35 d | 77 d | 119 d |
Days after MS delivery | 24 h | 72 h | 7 d | 14 d | 3 wks | 9 wks | 15 wks | ||||
ELISA test | x | x | x | x | x | x | x | x | x | x | x |
RT-PCR | x | x | x | x | x | x | x | x | x |
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Bajdak-Rusinek, K.; Fus-Kujawa, A.; Buszman, P.; Żyła-Uklejewicz, D.; Jelonek, K.; Musiał-Kulik, M.; Fernandez, C.; Michalak, M.; George, K.; Kasperczyk, J.; et al. SDF-1α-Releasing Microspheres Effectively Extend Stem Cell Homing after Myocardial Infarction. Biomedicines 2023, 11, 343. https://doi.org/10.3390/biomedicines11020343
Bajdak-Rusinek K, Fus-Kujawa A, Buszman P, Żyła-Uklejewicz D, Jelonek K, Musiał-Kulik M, Fernandez C, Michalak M, George K, Kasperczyk J, et al. SDF-1α-Releasing Microspheres Effectively Extend Stem Cell Homing after Myocardial Infarction. Biomedicines. 2023; 11(2):343. https://doi.org/10.3390/biomedicines11020343
Chicago/Turabian StyleBajdak-Rusinek, Karolina, Agnieszka Fus-Kujawa, Piotr Buszman, Dorota Żyła-Uklejewicz, Katarzyna Jelonek, Monika Musiał-Kulik, Carlos Fernandez, Magdalena Michalak, Kurian George, Janusz Kasperczyk, and et al. 2023. "SDF-1α-Releasing Microspheres Effectively Extend Stem Cell Homing after Myocardial Infarction" Biomedicines 11, no. 2: 343. https://doi.org/10.3390/biomedicines11020343