Angiotensin II Modulates Calcium/Phosphate Excretion in Experimental Model of Hypertension: Focus on Bone

Castoldi, Giovanna; Carletti, Raffaella; Ippolito, Silvia; Villa, Isabella; Palmisano, Biagio; Bolamperti, Simona; Rubinacci, Alessandro; Zerbini, Gianpaolo; Meani, Michela; Zatti, Giovanni; di Gioia, Cira R. T.

doi:10.3390/biomedicines10112928

Open AccessArticle

Angiotensin II Modulates Calcium/Phosphate Excretion in Experimental Model of Hypertension: Focus on Bone

by

Giovanna Castoldi

^1,*,

Raffaella Carletti

²,

Silvia Ippolito

³,

Isabella Villa

⁴

,

Biagio Palmisano

⁵

,

Simona Bolamperti

⁴

,

Alessandro Rubinacci

⁶

,

Gianpaolo Zerbini

⁷

,

Michela Meani

^1,8,

Giovanni Zatti

^1,8 and

Cira R. T. di Gioia

⁹

¹

Dipartimento di Medicina e Chirurgia, Università degli Studi di Milano-Bicocca, 20900 Monza, Italy

²

Dipartimento di Medicina Traslazionale e di Precisione, Sapienza Università di Roma, 00185 Rome, Italy

³

Laboratorio Analisi Chimico Cliniche, Ospedale San Gerardo, ASST Monza, 20900 Monza, Italy

⁴

Laboratorio di Endocrinologia e Metabolismo Osseo, Istituto di Endocrinologia e Scienze Metaboliche, IRCCS Ospedale San Raffaele, 20132 Milano, Italy

⁵

Dipartimento di Medicina Molecolare, Sapienza Università di Roma, 00161 Roma, Italy

⁶

Osteoporosis and Bone and Mineral Metabolism Unit, IRCCS Ospedale San Raffaele, 20132 Milano, Italy

⁷

Unita’ Complicanze del Diabete, IRCCS Ospedale San Raffaele, 20132 Milano, Italy

⁸

Clinica Ortopedica, Ospedale San Gerardo, ASST Monza, 20900 Monza, Italy

⁹

Dipartimento di Scienze Radiologiche, Oncologiche e Anatomopatologiche, Istituto di Anatomia Patologica, Sapienza Università di Roma, 00161 Rome, Italy

^*

Author to whom correspondence should be addressed.

Biomedicines 2022, 10(11), 2928; https://doi.org/10.3390/biomedicines10112928

Submission received: 2 October 2022 / Revised: 4 November 2022 / Accepted: 8 November 2022 / Published: 14 November 2022

(This article belongs to the Special Issue Animal Models for Study of Pathophysiological Mechanisms of Hypertension and Its Complications)

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Abstract

A link between hypertension and long-term bone health has been suggested. The aim of this study was to investigate the effects of chronic angiotensin II administration on urinary calcium/phosphate excretion, bone mineral density, bone remodeling and osteoblast population in a well-established experimental model of hypertension, in the absence of possible confounding factors that could affect bone metabolism. Male Sprague–Dawley rats, divided in the following groups: (a) Angiotensin II (Ang II, 200 ng/kg/min, osmotic minipumps, sub cutis, n = 8); (b) Ang II+losartan (Los, 50 mg/kg/day, per os, n = 6); (c) control group (physiological saline, sub cutis, n = 9); and (d) control+losartan (n = 6) were treated for four weeks. During the experimental period, 24-hour diuresis, urinary calcium, phosphate and sodium excretion were measured prior to the treatment, at two weeks of treatment, and at the end of the treatment. Systolic blood pressure was measured by plethysmography technique (tail cuff method). At the end of the experimental protocol, the rats were euthanized and peripheral quantitative computed tomography at the proximal metaphysis and at the diaphysis of the tibiae and quantitative bone histomorphometry on distal femora were performed. Angiotensin II-dependent hypertension is associated with increased calcium and phosphate excretion. AT1 receptor blockade prevented the increase of blood pressure and phosphate excretion but did not affect the increase of calcium excretion. These changes took place without significantly affecting bone density, bone histology or osteoblast population. In conclusion, in our experimental conditions, angiotensin II-dependent hypertension gave rise to an increased urinary excretion of calcium and phosphate without affecting bone density.

Keywords: angiotensin II; experimental hypertension; bone; rats

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MDPI and ACS Style

Castoldi, G.; Carletti, R.; Ippolito, S.; Villa, I.; Palmisano, B.; Bolamperti, S.; Rubinacci, A.; Zerbini, G.; Meani, M.; Zatti, G.; et al. Angiotensin II Modulates Calcium/Phosphate Excretion in Experimental Model of Hypertension: Focus on Bone. Biomedicines 2022, 10, 2928. https://doi.org/10.3390/biomedicines10112928

AMA Style

Castoldi G, Carletti R, Ippolito S, Villa I, Palmisano B, Bolamperti S, Rubinacci A, Zerbini G, Meani M, Zatti G, et al. Angiotensin II Modulates Calcium/Phosphate Excretion in Experimental Model of Hypertension: Focus on Bone. Biomedicines. 2022; 10(11):2928. https://doi.org/10.3390/biomedicines10112928

Chicago/Turabian Style

Castoldi, Giovanna, Raffaella Carletti, Silvia Ippolito, Isabella Villa, Biagio Palmisano, Simona Bolamperti, Alessandro Rubinacci, Gianpaolo Zerbini, Michela Meani, Giovanni Zatti, and et al. 2022. "Angiotensin II Modulates Calcium/Phosphate Excretion in Experimental Model of Hypertension: Focus on Bone" Biomedicines 10, no. 11: 2928. https://doi.org/10.3390/biomedicines10112928

APA Style

Castoldi, G., Carletti, R., Ippolito, S., Villa, I., Palmisano, B., Bolamperti, S., Rubinacci, A., Zerbini, G., Meani, M., Zatti, G., & di Gioia, C. R. T. (2022). Angiotensin II Modulates Calcium/Phosphate Excretion in Experimental Model of Hypertension: Focus on Bone. Biomedicines, 10(11), 2928. https://doi.org/10.3390/biomedicines10112928

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Angiotensin II Modulates Calcium/Phosphate Excretion in Experimental Model of Hypertension: Focus on Bone

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