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Article

A Simple and Rapid Spectrophotometric Method for Nitrite Detection in Small Sample Volumes

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Division of Health and Applied Sciences, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand
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Forensic Innovation Center, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand
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Center of Excellence for Trace Analysis and Biosensors (TAB-CoE), Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand
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Center of Excellence for Innovation in Chemistry, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand
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Forensic Science Programme, School of Health Sciences, Universiti Sains Malaysia, Kubang Kerian 16150, Kelantan, Malaysia
*
Author to whom correspondence should be addressed.
Academic Editor: Yoav Broza
Chemosensors 2021, 9(7), 161; https://doi.org/10.3390/chemosensors9070161
Received: 18 May 2021 / Revised: 17 June 2021 / Accepted: 22 June 2021 / Published: 25 June 2021
A simple, rapid, and environmentally-friendly spectrophotometric method for nitrite detection was developed. Detection was based on a redox reaction with iodide ions in an acidic condition. The reaction was evaluated by detecting the increase in absorbance of the colored product of iodine at 362 nm wavelength. To obtain a good spectrophotometric performance, the iodide ions concentration, hydrochloric acid concentration, and reaction time were optimized. In the optimal condition, the developed spectrophotometric method provided a linear range of 0.0625 to 4.00 mg L−1 (r = 0.9985), reaction time for 10 min, a limit of detection of 25 µg L−1, and a limit of quantitation of 85 µg L−1. This method showed good repeatability (RSD < 9.21%), high sample throughput (9 samples min−1), and good accuracy (recovery = 88 ± 2 to 99.5 ± 0.4%). The method has the potential to be used in crime scene investigations as a rapid screening test for gunshot residue detection via nitrite detection. View Full-Text
Keywords: nitrite; spectrophotometric method; 96 well microplate nitrite; spectrophotometric method; 96 well microplate
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MDPI and ACS Style

Thipwimonmas, Y.; Jaidam, J.; Samoson, K.; Khunseeraksa, V.; Phonchai, A.; Thiangchanya, A.; Chang, K.H.; Abdullah, A.F.L.; Limbut, W. A Simple and Rapid Spectrophotometric Method for Nitrite Detection in Small Sample Volumes. Chemosensors 2021, 9, 161. https://doi.org/10.3390/chemosensors9070161

AMA Style

Thipwimonmas Y, Jaidam J, Samoson K, Khunseeraksa V, Phonchai A, Thiangchanya A, Chang KH, Abdullah AFL, Limbut W. A Simple and Rapid Spectrophotometric Method for Nitrite Detection in Small Sample Volumes. Chemosensors. 2021; 9(7):161. https://doi.org/10.3390/chemosensors9070161

Chicago/Turabian Style

Thipwimonmas, Yudtapum, Janjira Jaidam, Kritsada Samoson, Vacharachai Khunseeraksa, Apichai Phonchai, Adul Thiangchanya, Kah H. Chang, Ahmad F.L. Abdullah, and Warakorn Limbut. 2021. "A Simple and Rapid Spectrophotometric Method for Nitrite Detection in Small Sample Volumes" Chemosensors 9, no. 7: 161. https://doi.org/10.3390/chemosensors9070161

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