Handheld Enzymatic Luminescent Biosensor for Rapid Detection of Heavy Metals in Water Samples
by
Kirill A. Lukyanenko 1,2,†
, Ivan A. Denisov 1,2,*,†, Vladimir V. Sorokin 2,†, Anton S. Yakimov 3, Elena N. Esimbekova 2,4 and Peter I. Belobrov 2
Kirill A. Lukyanenko 1,2,†, Ivan A. Denisov 1,2,*,†, Vladimir V. Sorokin 2,†, Anton S. Yakimov 3, Elena N. Esimbekova 2,4 and Peter I. Belobrov 2
1
Laboratory for Digital Controlled Drugs and Theranostics, Federal Research Center “Krasnoyarsk Science Center SB RAS”, Krasnoyarsk 660036, Russia
2
Laboratory of Bioluminescent Biotechnologies, Siberian Federal University, Krasnoyarsk 660041, Russia
3
Research Institute of Molecular Medicine and Pathobiochemistry, Krasnoyarsk State Medical University named after Prof. V.F. Voino-Yasenetsky, Krasnoyarsk 660022, Russia
4
Laboratory of Photobiology, Institute of Biophysics SB RAS, Krasnoyarsk 660036, Russia
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Chemosensors 2019, 7(1), 16; https://doi.org/10.3390/chemosensors7010016
Received: 15 February 2019 / Revised: 18 March 2019 / Accepted: 19 March 2019 / Published: 26 March 2019
(This article belongs to the Special Issue Chemical Sensors for Heavy Metals/Toxin Detection)
Enzymatic luminescent systems are a promising tool for rapid detection of heavy metals ions for water quality assessment. Nevertheless, their widespread use is limited by the lack of test procedure automation and available sensitive handheld luminometers. Herein we describe integration of disposable microfluidic chips for bioluminescent enzyme-inhibition based assay with a handheld luminometer, which detection system is based on a thermally stabilized silicon photomultiplier (SiPM). Microfluidic chips were made of poly(methyl methacrylate) by micro-milling method and sealed using a solvent bonding technique. The composition of the bioluminescent system in microfluidic chip was optimized to achieve higher luminescence intensity and storage time. Results indicate that developed device provided comparable sensitivity with bench-scale PMT-based commercial luminometers. Limit of detection for copper (II) sulfate reached 2.5 mg/L for developed biosensor. Hereby we proved the concept of handheld enzymatic optical biosensors with disposable chips for bioassay. The proposed biosensor can be used as an early warning field-deployable system for rapid detection of heavy metals salts and other toxic chemicals, which affect bioluminescent signal of enzymatic reaction.
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Keywords:
chemical measurements; silicon photomultiplier; optical biosensor; bioassay; microfluidics; luciferase; bioluminescence
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MDPI and ACS Style
Lukyanenko, K.A.; Denisov, I.A.; Sorokin, V.V.; Yakimov, A.S.; Esimbekova, E.N.; Belobrov, P.I. Handheld Enzymatic Luminescent Biosensor for Rapid Detection of Heavy Metals in Water Samples. Chemosensors 2019, 7, 16.
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