Next Article in Journal
Real-Time Frequency Tracking of an Electro-Thermal Piezoresistive Cantilever Resonator with ZnO Nanorods for Chemical Sensing
Previous Article in Journal
Resistive Low-Temperature Sensor Based on the SiO2ZrO2 Film for Detection of High Concentrations of NO2 Gas
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Chemosensors
Volume 7
Issue 1
10.3390/chemosensors7010001
chemosensors-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles thumb_up
...
Endorse textsms
...
Comment
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Electrochemically Prepared Unzipped Single Walled Carbon Nanotubes-MnO2 Nanostructure Composites for Hydrogen Peroxide and Glucose Sensing

by
A. B. M. Zakaria
1,† and
Danuta Leszczynska
2,*
1
Department of Chemistry and Biochemistry, Jackson State University, Jackson, MS 39217, USA
2
Department of Civil and Environmental Engineering and Interdisciplinary Center of Nanotoxicity, Jackson State University, Jackson, MS 39217, USA
*
Author to whom correspondence should be addressed.
†
Current address: Department of Radiology and Biomedical Imaging, Yale School of Medicine, New Haven, CT 06520, USA.
Chemosensors 2019, 7(1), 1; https://doi.org/10.3390/chemosensors7010001
Submission received: 14 November 2018 / Revised: 21 December 2018 / Accepted: 24 December 2018 / Published: 3 January 2019
Browse Figures
Versions Notes

Abstract

:
Amperometric hydrogen peroxide (H2O2) and glucose biosensors based on unzipped carbon nanotubes with modified glassy carbon electrode (GCE) have been successfully fabricated via a facile electrochemical oxidative method. In this work, we investigated the feasibility of this new form of carbon nanomaterial as a substrate electrode material for fabricating sensitive platform for H2O2 and glucose sensors. For this purpose, the manganese oxide (MnO2)/unzipped single-walled carbon nanotubes (SWCNTs) film was synthesized by the cyclic voltammetry method. The developed sensing film, MnO2/unzipped SWCNTs/GCE, displayed a satisfactory analytical performance for H2O2, including a wide linear range of 2.0 × 10−6 to 5.0 × 10−3 M with a detection limit of 0.31 × 10−6 M (10.7 ppb). This film was further applied for glucose sensing with a linearity range of 0.01 to 1.2 mM with a correlation coefficient of 0.9822 in the physiological pH (7.4). This facile, fast, environmentally-friendly, and economical preparation strategy of carbon nanomaterial-based electrode materials opens up the possibility of developing high quality biocompatible hydrogen peroxide and glucose sensors.

Graphical Abstract

1. Introduction

Unzipped carbon nanotubes, a new transformed material commonly synthesized from carbon nanotubes (CNTs), are a combination of one-dimensional CNTs and two-dimensional graphene [1]. This unique nanostructure has also been called graphene nanoribbons (GNRs) [2,3]. It has attracted enormous interest from both theoretical and experimental scientists due to its unique electrical [4], optical [5], thermal [6], doping [7], magnetic [8], and mechanical [9] properties. To date, numerous experiments have been performed to categorize the practical significance in manufacturing devices, such as field-effect transistors [10], photodetectors [11], catalysts for proton exchange membrane (PEM) fuel cells [12], and magnetic field sensors [13]. However, very few reports have been published on using this carbon nanostructure as a substrate material for constructing sensors for specific measurements, such as hydrogen peroxide and glucose.
Various preparation methods for unzipping carbon nanotubes are known, such as plasma etching [14], annealing in hydrogen [15], intercalation–exfoliation [16], catalytic metal nanoparticle cutting [17], and chemical unzipping [18,19]. Among these methods, the electrochemical approach is an effective way for converting carbon nanotubes (CNTs) to unzipped carbon nanotubes. Moreover, the electrochemical method has a unique advantage when compared with other methods, as it allows controlling of the layer’s thickness and orientation [20,21,22]. Graphene-like unzipped carbon nanotubes consist of a sp2 carbon plane, but have an elongated structure with a high aspect ratio, edge density, and defects sites, as well as high-quality graphene layers. These unique properties make them an excellent candidate for electrochemical sensing platforms with high current responses, and lower limits of detection towards target analytes.
Novel metal nanostructures incorporating carbon nanomaterials (CNMs) have shown great potential applications in the fields of electrochemical sensing devices. Recently, metal and transition metal oxide nanoparticles have been extensively applied in developing high efficient electrochemical sensing toward H2O2 and glucose [23,24,25]. Particularly, manganese oxide (MnO2) nanoparticles are one of the most promising inorganic materials in fabricating sensing devices due to high catalytic ability, low-cost, high energy density, and environmental benignity [26,27,28,29].
Nowadays, H2O2 and glucose are suitable probe analytes to justify a new material in future biosensing applications. Several new forms of hybrid materials have been investigated by using these probe analytes. Most importantly, CNM-based sensors possess fast electron transfer kinetics, low limits of detection, and high sensitivity. For example, Yaojuan et al. investigated gold nanoparticle (AuNP)–graphene nanocomposites as a new electrode material synthesized by electrochemical deposition of Au–NPs on graphene sheets [30]. Selvakumar et al. demonstrated the glucose biosensing properties of a direct growth of glucose oxidase (GOx) on reduced graphene oxide/β-Cyclodextrin composite modified electrode for glucose monitoring [31]. Qinglin et al. fabricated glassy carbon electrode with graphene–carbon nanotube–nafion/gold–platinum alloy nanoparticles [32]. Nur et al. used Au nanoparticles supported graphene oxide nanoribbons (GONRs) [33]. Minmin et al. demonstrated Cu2O nanocubes wrapped in graphene nanosheets (Cu2O/GNs) as electrocatalysts [34]. Jasmina et al. reported an amperometric H2O2 sensor using a carbon paste electrode (CPE) coated with platinum nanoparticles on graphitized carbon (Pt–C) [35].
The preparation of these electrode materials is usually time-consuming and high-cost, and requires complicated, multi-step procedures. Therefore, a simple, low-cost preparation method to fabricate electrode is highly desired. In our previous work, we have achieved substantial progress on preparing substrate electrode material including synthesis and characterization of unzipped single-walled carbon nanotubes (unzipped SWCNTs) [36]. The obtained new form of carbon nanomaterial offers high active sites, fast electron transfer kinetics, and effective high surface area.
In this work, we report our extended study on preparing the final electrode material by the electrodeposition of MnO2 on as-prepared unzipped SWCNTs modified glassy carbon electrode (GCE) and evaluating its electrocatalytic performances towards electrooxidation of the probe analytes, such as H2O2 and glucose.

2. Materials and Methods

2.1. Reagents and Apparatus

Single-walled carbon nanotubes (non-functionalized, purity >90 wt.%, outer diameter 1–2 nm, length 5–30 µm) were purchased from Cheap Tubes Inc. Cambridgeport, VT, USA. Nafion (perfluorinated ion-exchange resin), sulfuric acid (93–98 w/w%, density 1.84 g/cm3 at 20 °C), Na2HPO4·7H2O, KH2PO4, NaCl, KCl, glucose oxidase (from Aspergillus niger, 18,200 units g−1), and glucose (D-(+)-99.5%) were purchased from Sigma-Aldrich (St. Louis, MO, USA). All chemicals were analytical grade and were used as received. All stock solutions were prepared using deionized water without further purification.
Amperometric and cyclic voltammograms were carried out with CH Instruments (CHI 440, CH Instruments, Austin, TX, USA) using a standard three-electrode system. The glassy carbon electrode (GCE), Ag/AgCl/saturated KCl (Model CH111, CH Instruments, Austin, TX, USA), and a platinum wire were used as working, reference, and counter electrodes, respectively. High purity argon was used to deoxygenate all experimental solutions for 20 min, and maintaining argon atmosphere during experiments. Scanning electron microscopy (ZEISS Supra 40VP SEM) and transmission electron microscopy (JEOL-2100F operated at 100 kV) were used for characterizing the surface morphology and nanostructure of the carbon nanomaterials and MnO2 nanoparticles.

2.2. Preparation of MnO2/Unzipped SWCNTs/GCE

Unzipped SWCNTs/GCE was prepared as reported before [20,36]. Briefly, 5 mg of SWCNTs was dispersed in 5 mL of Nafion under 20 min constant ultrasonic agitations until obtaining 1 mg/mL of black homogeneous suspension. The glassy carbon electrode was polished with 0.05 µm alumina powder, and washed ultrasonically in deionized water and ethanol for 10 min. Then, a 3 µL of the black suspension was dropped onto cleaned GCE surface, and dried under a yellow lamp at 50 °C to obtain the SWCNTs/GCE.
The modified GCE was subjected to electrochemical oxidation in potential window of −0.2 to 0.7 V, at scan rate 100 mVs−1 in 0.5 M H2SO4 for 12 h. Then, MnO2 was electrodeposited on the surface of unzipped SWCNTs/GCE by repetitive scanning cycles by applying potential window of 0.3 to 1.3 V in a solution of 0.1 M Na2SO4 and 5.0 mM MnSO4. The electrochemical setup, including the steps of preparation method of modified glassy carbon electrode (GCE) and electrochemical reactions in sensing H2O2 and glucose, is illustrated in Figure 1.

3. Results and Discussion

3.1. Characterization of Unzipped SWCNTs and MnO2 Nanostructured Film

The surface of SWCNTs was investigated before and after electrochemical treatment by scanning electron microscopy (SEM). Figure 2A shows the SEM image of the characteristic tubular networks of pristine SWCNTs. After anodic oxidation, a cloudy surface morphology of CNT was observed (Figure 2B). This cloudy feature of unzipped SWCNTs was observed due to increasing edge carbon resulting hydrophilic nature developing on its surface and attaching atmospheric water molecules.
In addition, Figure 2C,D depicts the spike-like MnO2 anchored with edge carbon generated during transformation of SWCNTs to unzipped SWCNTs. The homogeneous structure of MnO2/unzipped SWCNTs film suggests that MnO2 was successfully attached on the surface of unzipped SWCNTs.
In order to confirm the oxidative opening (unzipping) of SWCNTs, we carried out Fourier transform infrared spectroscopy (FTIR) and Raman spectroscopy analyses. The FTIR (Figure 3A) spectrum of unzipped SWCNTs illustrates higher absorption of O–H group in the regions of O–H (3000–3700 cm−1) and C=O modes (1700–1750 cm−1), and lower absorption in the regions of C–O (1000–1250 cm−1) and C=C–H (800–1000 cm−1) compared to the spectrum of pristine SWCNTs. Moreover, Raman spectra (Figure 3B) also confirmed the fully unzipping of pristine SWCNTs. The G and D bands of SWCNTs has been reported as the characteristics of sp2 carbon atoms and the extent of defects of carbon nanostructure respectively [37]. The spectrum of unzipped SWCNTs in Figure 3B shows the intensity of the D band at 1360 cm−1 and the G band at 1600 cm−1 s increased and broadened compared to the pristine SWCNTs. This observation is attributed to the confirmation of the formation of unzipped SWCNTs.

3.2. Formation of MnO2 on Unzipped SWCNTs/GCE

Figure 4 shows the voltammograms of the first scan and scans after 12 h and 72 h, respectively, indicating the stable and successful unzipping of carbon nanotubes.
Figure 5A shows the cyclic voltammograms (CVs) obtained in solution of 0.1M Na2SO4 and 5.0 mM MnSO4 at SWCNTs/GCE and unzipped SWCNTs/GCE. Obtained data strongly demonstrated that the sharp and large peak area of oxidizing Mn2+ to Mn3+ and reducing Mn3+ to Mn2+ was observed at unzipping SWCNTs when compared to pristine SWCNTs. The current intensity of oxidation and reduction during electrodeposition of MnO2 was increased with repetitive voltammetric cycles (Figure 5B). This observation is consistent with our previous work [27]. It also suggests that large amounts of MnO2 nanoparticles were successfully attached due to enlarged surface area with increasing active sites on unzipped SWCNTs.

3.3. Electrocatalytic Oxidation of H2O2 at MnO2/Unzipped SWCNTs/GCE

Figure 6A displays the cyclic voltammetry (CV) obtained at the pristine SWCNTs/GCE (a), only MnO2/GCE (b), unzipped SWCNTs/GCE (c), and MnO2/unzipped SWCNTs/GCE (d) in 0.1 M PBS (pH 7.4) containing 1.0 mM H2O2, at a scan rate of 100 mVs−1. The observed electrocatalytic ability of these different electrodes towards oxidation of H2O2 has shown following order: MnO2/unzipped SWCNTs/GCE > unzipped SWCNTs/GCE > MnO2/GCE > Mn pristine SWCNTs/GCE.
The pristine SWCNTs did not show any oxidation peak indicating its electrochemical inactivity in this potential window. The unzipped SWCNTs modified GCE showed some oxidation current due to available active sites generated during the opening of the side wall of the carbon nanotubes. In contrast, a noticeable oxidation peak at the potential of 0.85 V was observed at MnO2/unzipped SWCNTs/GCE resulting from two factors. Firstly, MnO2 has high catalytic activity for H2O2.
Secondly, spike or edge-like structure of MnO2 nanoparticles electrodeposited on unzipped SWCNTs might have higher catalytic ability for decomposing H2O2 than regular-sized nanoparticles. From Figure 6B, it is obvious that the oxidation peak current was proportional to increasing H2O2 concentration (a–f: 1.0 mM to 5.0 mM). The excellent electrochemical activity toward H2O2 can be attributed to the relatively high specific surface area, and the orientation of nanostructured MnO2 on the unzipped SWCNTs/GCE surface.

3.4. Determination of H2O2

Figure 7 displays the amperometric current–time curve of MnO2/unzipped SWCNTs/GCE for the successive addition of H2O2 in 0.1 M PBS (pH 7.4) at the potential +0.85 V. The figure (inset) also shows the calibration curve, and the regression equation of the sensor. The regression equation is as follows:
Ip(μA) = 0.0209x + 1.7781
where x is the concentration of H2O2 in μM.
The linear range of this calibration curve was from 2.0 × 10−6 to 5.0 × 10−3 M with a correlation coefficient of 0.9831. From the slope of 0.0209 μA/μM, the limit of detection (LOD) was calculated to 0.31 × 10−6 M using the following equation:
LOD = SDbackground/S
where S is slop or sensitivity, SD: Standard deviation.
The linearity range and sensitivity for detecting H2O2 at MnO2/unzipped SWCNTs/GCE was greater than that of MnO2/pristine SWCNTs/GCE, as was reported in our previous work [30]. In addition, the sensor exhibited appreciable reproducibility of 3.78% for five independent measurements carried out by five different MnO2/unzipped SWCNT film modified electrodes.
Table 1 summarizes performance of different modified electrodes toward direct measurements of hydrogen peroxide in water matrix.

3.5. Application of MnO2/Unzipped SWCNTs/GCE in Glucose Sensing

Glucose oxidase (GOx) was taken as the representative enzyme to investigate the electrocatalytic ability of MnO2/unzipped SWCNTs modified GCE during glucose monitoring. As is well-known, glucose oxidase catalyzes the oxidation of glucose with oxygen, accompanying the production of gluconic acid and H2O2. Consequently, the concentration of glucose can be indirectly detected by the determination of the liberated H2O2 during the enzymatic catalysis. Figure 8 shows the amperometric response of the MnO2/unzipped SWCNTs/GCE during successive addition of glucose to a solution containing 1 mg/1 mL GOx. Based on the current response towards the oxidation of H2O2 during the enzyme/glucose reaction, the calibration curve was plotted between catalytic current and glucose concentration. The linearity range was estimated from 0.01 × 10−3 to 1.2 × 10−3 M with a correlation coefficient of 0.9822 and the detection limit of the glucose was 1.2 × 10−6 M. Table 2 summarizes performance of several composite electrodes toward glucose detection, including our work.

3.6. Selectivity, Stability and Reproducibility Property of the MnO2/Unzipped SWCNTs/GCE

The prepared electrode showed higher stability for amperometric measurement at a constant potential. Figure 9A displays the stability response without addition of glucose to 0.1 M, pH 7.4, PBS solution containing 1 mg/mL GOx at 0.85 V (vs. Ag/AgCl). The reproducibility of the as-prepared electrode was also evaluated by measuring amperometric (Eap = 0.85 V) responses of the sequential addition of 0.1 mM of glucose in 0.1M PBS. The relative standard deviation (R.S.D) was 5.4% suggesting the high reproducibility of the prepared electrode. Moreover, Figure 9B shows the amperometric response of the sequential addition of 0.1 mM glucose, and the common interferents including uric acid (UA) and ascorbic acid (AA) of 0.1 mM for each addition. The significant response for glucose and insignificant responses for interfering species were observed, suggesting good selectivity for glucose detection.

4. Conclusions

We have outlined a facile methodology for constructing a suitable electrochemical sensing platform based on MnO2/unzipped SWCNTs that exhibits high electrocatalytic activity toward the oxidation of H2O2 in physiological pH of 7.4. Electrochemical method presents an efficient approach for tunable, simple and fast preparation of MnO2/unzipped SWCNTs film. More importantly, electrochemically synthesized unzipped SWCNTs provided sufficient anchoring sites that allowed the formation of high electrocatalytic MnO2 nanostructured film. Consequently, the as-prepared MnO2/unzipped SWCNTs can be used for monitoring H2O2 at a low potential of 0.85 V with high sensitivity. The estimated linearity range for measuring H2O2 was found to be from 2.0 × 10−6 to 5.0 × 10−3 M and the detection limit was 0.31 × 10−6 M. Furthermore, this modified electrode was detecting very low concentration (1.2 × 10−6 M) of glucose. The results show that MnO2/unzipped SWCNTs are good nanostructured materials that can be applied for developing biosensors for monitoring different biological analytes.

Author Contributions

There is a balanced contribution to this study from two co-authors; Conceptualization, A.B.M.Z. and D.L.; Investigation, A.B.M.Z. and D.L.; Supervision, D.L.; Writing—original draft, A.B.M.Z.; Writing—review & editing, D.L.

Funding

This study was financially supported by the National Science Foundation (grants: EPS-362492-190200-01; EPS-0903787; and HRD 1547754; RCMI-8G12MD007581).

Acknowledgments

The authors would like to offer special thanks to Hiroyasu Tachikawa for using his electrochemical workstation.

Conflicts of Interest

The authors declare no conflict of interest.

References

  1. Kim, N.D.; Li, Y.; Wang, G.; Fan, X.; Jiang, J.; Li, L.; Ji, Y.; Ruan, G.; Hauge, R.H.; Tour, J.M. Growth and Transfer of Seamless 3D Graphene–Nanotube Hybrids. Nano Lett. 2016, 16, 1287–1292. [Google Scholar] [CrossRef] [PubMed]
  2. Kosynkin, D.V.; Higginbotham, A.L.; Sinitskii, A.; Lomeda, J.R.; Dimiev, A.; Price, B.K.; Tour, J.M. Longitudinal unzipping of carbon nanotubes to form graphene nanoribbons. Nature 2009, 458, 872–876. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  3. John, R.; Shinde, D.B.; Liu, L.; Ding, F.; Xu, Z.; Vijayan, C.; Pillai, V.K.; Pradeep, T. Sequential Electrochemical Unzipping of Single-Walled Carbon Nanotubes to Graphene Ribbons Revealed by in Situ Raman Spectroscopy and Imaging. ACS Nano 2014, 8, 234–242. [Google Scholar] [CrossRef] [PubMed]
  4. Li, Y.; Zhou, W.; Wang, H.; Xie, L.; Liang, Y.; Wei, F.; Idrobo, J.-C.; Pennycook, S.J.; Dai, H. An oxygen reduction electrocatalyst based on carbon nanotube-graphene complexes. Nat. Nano 2012, 7, 394–400. [Google Scholar] [CrossRef] [PubMed]
  5. Deilmann, T.; Rohlfing, M. Huge Trionic Effects in Graphene Nanoribbons. Nano Lett. 2017, 17, 6833–6837. [Google Scholar] [CrossRef]
  6. Chen, X.; Xu, Y.; Zou, X.; Gu, B.-L.; Duan, W. Interfacial thermal conductance of partially unzipped carbon nanotubes: Linear scaling and exponential decay. Phys. Rev. B 2013, 87, 155438. [Google Scholar] [CrossRef]
  7. Ervasti, M.M.; Fan, Z.; Uppstu, A.; Krasheninnikov, A.V.; Harju, A. Silicon and silicon-nitrogen impurities in graphene: Structure, energetics, and effects on electronic transport. Phys. Rev. B 2015, 92, 235412. [Google Scholar] [CrossRef]
  8. Chen, X.; Zhang, L.; Guo, H. Valley caloritronics and its realization by graphene nanoribbons. Phys. Rev. B 2015, 92, 155427. [Google Scholar] [CrossRef]
  9. Yan, Z.; Peng, Z.; Casillas, G.; Lin, J.; Xiang, C.; Zhou, H.; Yang, Y.; Ruan, G.; Raji, A.-R.O.; Samuel, E.L.G.; et al. Rebar Graphene. ACS Nano 2014, 8, 5061–5068. [Google Scholar] [CrossRef]
  10. Candini, A.; Martini, L.; Chen, Z.; Mishra, N.; Convertino, D.; Coletti, C.; Narita, A.; Feng, X.; Müllen, K.; Affronte, M. High Photoresponsivity in Graphene Nanoribbon Field-Effect Transistor Devices Contacted with Graphene Electrodes. J. Phys. Chem. C 2017, 121, 10620–10625. [Google Scholar] [CrossRef] [Green Version]
  11. Wei, D.; Xie, L.; Lee, K.K.; Hu, Z.; Tan, S.; Chen, W.; Sow, C.H.; Chen, K.; Liu, Y.; Wee, A.T.S. Controllable unzipping for intramolecular junctions of graphene nanoribbons and single-walled carbon nanotubes. Nat. Commun. 2013, 4, 1374. [Google Scholar] [CrossRef] [Green Version]
  12. Long, D.; Li, W.; Qiao, W.; Miyawaki, J.; Yoon, S.-H.; Mochida, I.; Ling, L. Partially unzipped carbon nanotubes as a superior catalyst support for PEM fuel cells. Chem. Commun. 2011, 47, 9429–9431. [Google Scholar] [CrossRef] [PubMed]
  13. Costamagna, S.; Schulz, A.; Covaci, L.; Peeters, F. Partially unzipped carbon nanotubes as magnetic field sensors. Appl. Phys. Lett. 2012, 100, 232104. [Google Scholar] [CrossRef] [Green Version]
  14. Jiao, L.; Zhang, L.; Wang, X.; Diankov, G.; Dai, H. Narrow graphene nanoribbons from carbon nanotubes. Nature 2009, 458, 877–880. [Google Scholar] [CrossRef] [PubMed]
  15. Talyzin, A.V.; Luzan, S.; Anoshkin, I.V.; Nasibulin, A.G.; Jiang, H.; Kauppinen, E.I.; Mikoushkin, V.M.; Shnitov, V.V.; Marchenko, D.E.; Noréus, D. Hydrogenation, Purification, and Unzipping of Carbon Nanotubes by Reaction with Molecular Hydrogen: Road to Graphane Nanoribbons. ACS Nano 2011, 5, 5132–5140. [Google Scholar] [CrossRef] [PubMed]
  16. Kosynkin, D.V.; Lu, W.; Sinitskii, A.; Pera, G.; Sun, Z.; Tour, J.M. Highly Conductive Graphene Nanoribbons by Longitudinal Splitting of Carbon Nanotubes Using Potassium Vapor. ACS Nano 2011, 5, 968–974. [Google Scholar] [CrossRef] [PubMed]
  17. Elías, A.L.; Botello-Méndez, A.R.; Meneses-Rodríguez, D.; Jehová González, V.; Ramírez-González, D.; Ci, L.; Munoz-Sandoval, E.; Ajayan, P.M.; Terrones, H.; Terrones, M. Longitudinal cutting of pure and doped carbon nanotubes to form graphitic nanoribbons using metal clusters as nanoscalpels. Nano Lett. 2009, 10, 366–372. [Google Scholar] [CrossRef]
  18. Jiao, L.; Wang, X.; Diankov, G.; Wang, H.; Dai, H. Facile synthesis of high-quality graphene nanoribbons. Nat. Nanotechnol. 2010, 5, 321–325. [Google Scholar] [CrossRef] [Green Version]
  19. Zehtab Yazdi, A.; Chizari, K.; Jalilov, A.S.; Tour, J.; Sundararaj, U. Helical and Dendritic Unzipping of Carbon Nanotubes: A Route to Nitrogen-Doped Graphene Nanoribbons. ACS Nano 2015, 9, 5833–5845. [Google Scholar] [CrossRef]
  20. Shinde, D.B.; Debgupta, J.; Kushwaha, A.; Aslam, M.; Pillai, V.K. Electrochemical Unzipping of Multi-walled Carbon Nanotubes for Facile Synthesis of High-Quality Graphene Nanoribbons. J. Am. Chem. Soc. 2011, 133, 4168–4171. [Google Scholar] [CrossRef]
  21. Guo, H.-L.; Wang, X.-F.; Qian, Q.-Y.; Wang, F.-B.; Xia, X.-H. A Green Approach to the Synthesis of Graphene Nanosheets. ACS Nano 2009, 3, 2653–2659. [Google Scholar] [CrossRef] [PubMed]
  22. Zakaria, A.B.M.; Vasquez, E.S.; Walters, K.B.; Leszczynska, D. Functional holey graphene oxide: A new electrochemically transformed substrate material for dopamine sensing. RSC Adv. 2015, 5, 107123–107135. [Google Scholar] [CrossRef]
  23. Palanisamy, S.; Cheemalapati, S.; Chen, S.-M. Amperometric glucose biosensor based on glucose oxidase dispersed in multiwalled carbon nanotubes/graphene oxide hybrid biocomposite. Mater. Sci. Eng. C 2014, 34, 207–213. [Google Scholar] [CrossRef] [PubMed]
  24. Unnikrishnan, B.; Palanisamy, S.; Chen, S.-M. A simple electrochemical approach to fabricate a glucose biosensor based on graphene–glucose oxidase biocomposite. Biosens. Bioelectron. 2013, 39, 70–75. [Google Scholar] [CrossRef] [PubMed]
  25. Palanisamy, S.; Chen, S.-M.; Sarawathi, R. A novel nonenzymatic hydrogen peroxide sensor based on reduced graphene oxide/ZnO composite modified electrode. Sens. Actuators B Chem. 2012, 166–167, 372–377. [Google Scholar] [CrossRef]
  26. Han, Y.; Zheng, J.; Dong, S. A novel nonenzymatic hydrogen peroxide sensor based on Ag–MnO2–MWCNTs nanocomposites. Electrochim. Acta 2013, 90, 35–43. [Google Scholar] [CrossRef]
  27. Zakaria, A.; Leszczynska, D. Novel design of non-enzymatic sensor for rapid monitoring of hydrogen peroxide in water matrix. J. Electroanal. Chem. 2016, 766, 30–36. [Google Scholar] [CrossRef] [Green Version]
  28. Vukojević, V.; Djurdjić, S.; Ognjanović, M.; Fabián, M.; Samphao, A.; Kalcher, K.; Stanković, D.M. Enzymatic glucose biosensor based on manganese dioxide nanoparticles decorated on graphene nanoribbons. J. Electroanal. Chem. 2018, 823, 610–616. [Google Scholar] [CrossRef]
  29. Schachl, K.; Alemu, H.; Kalcher, K.; Moderegger, H.; Svancara, I.; Vytras, K. Amperometric determination of hydrogen peroxide with a manganese dioxide film-modified screen printed carbon electrode. Fresenius J. Anal. Chem. 1998, 362, 194–200. [Google Scholar] [CrossRef]
  30. Hu, Y.; Jin, J.; Wu, P.; Zhang, H.; Cai, C. Graphene–gold nanostructure composites fabricated by electrodeposition and their electrocatalytic activity toward the oxygen reduction and glucose oxidation. Electrochim. Acta 2010, 56, 491–500. [Google Scholar] [CrossRef]
  31. Palanisamy, S.; Devasenathipathy, R.; Chen, S.-M.; Ajmal Ali, M.; Karuppiah, C.; Balakumar, V.; Prakash, P.; Elshikh, M.S.; Al-Hemaid, F.M.A. Direct Electrochemistry of Glucose Oxidase at Reduced Graphene Oxide and β-Cyclodextrin Composite Modified Electrode and Application for Glucose Biosensing. Electroanalysis 2015, 27, 2412–2420. [Google Scholar] [CrossRef]
  32. Sheng, Q.; Wang, M.; Zheng, J. A novel hydrogen peroxide biosensor based on enzymatically induced deposition of polyaniline on the functionalized graphene–carbon nanotube hybrid materials. Sens. Actuators B Chem. 2011, 160, 1070–1077. [Google Scholar] [CrossRef]
  33. Ismail, N.S.; Le, Q.H.; Yoshikawa, H.; Saito, M.; Tamiya, E. Development of Non-enzymatic Electrochemical Glucose Sensor Based on Graphene Oxide Nanoribbon—Gold Nanoparticle Hybrid. Electrochim. Acta 2014, 146, 98–105. [Google Scholar] [CrossRef]
  34. Liu, M.; Liu, R.; Chen, W. Graphene wrapped Cu2O nanocubes: Non-enzymatic electrochemical sensors for the detection of glucose and hydrogen peroxide with enhanced stability. Biosens. Bioelectron. 2013, 45, 206–212. [Google Scholar] [CrossRef] [PubMed]
  35. Anojčić, J.; Guzsvány, V.; Vajdle, O.; Kónya, Z.; Kalcher, K. Rapid amperometric determination of H2O2 by a Pt nanoparticle/Vulcan XC72 composite-coated carbon paste electrode in disinfection and contact lens solutions. Monatshefte Chem. Chem. Mon. 2018, 149, 1727–1738. [Google Scholar] [CrossRef]
  36. Zakaria, A.; Leszczynska, D.; Novikov, V. (Eds.) A Facial Electrochemical Method for Preparation and Characterization of Graphene Nanoribbons from Single Walled Carbon Nanotubes. In Mordern Directions in Chemistry, Biology, Pharmacy and Biotechnology; Lviv Polytechnic Publishing House: Lviv, Ukraine, 2015; pp. 110–116. [Google Scholar]
  37. Dresselhaus, M.S.; Dresselhaus, G.; Jorio, A.; Souza Filho, A.G.; Saito, R. Raman spectroscopy on isolated single wall carbon nanotubes. Carbon 2002, 40, 2043–2061. [Google Scholar] [CrossRef]
  38. Farid, M.M.; Goudini, L.; Piri, F.; Zamani, A.; Saadati, F. Molecular imprinting method for fabricating novel glucose sensor: Polyvinyl acetate electrode reinforced by MnO2/CuO loaded on graphene oxide nanoparticles. Food Chem. 2016, 194, 61–67. [Google Scholar] [CrossRef] [PubMed]
  39. Si, P.; Dong, X.-C.; Chen, P.; Kim, D.-H. A hierarchically structured composite of Mn3O4/3D graphene foam for flexible nonenzymatic biosensors. J. Mater. Chem. B 2013, 1, 110–115. [Google Scholar] [CrossRef]
  40. Ye, Y.; Wang, P.; Dai, E.; Liu, J.; Tian, Z.; Liang, C.; Shao, G. A novel reduction approach to fabricate quantum-sized SnO2-conjugated reduced graphene oxide nanocomposites as non-enzymatic glucose sensors. Phys. Chem. Chem. Phys. 2014, 16, 8801–8807. [Google Scholar] [CrossRef]
  41. Lin, K.-C.; Huang, L.-H.; Chen, S.-M. Electrochemical synthesis of mixed-valence manganese/copper hybrid composite using graphene oxide and multi-walled carbon nanotubes for nonenzymatic glucose sensor. J. Electroanal. Chem. 2014, 735, 36–42. [Google Scholar] [CrossRef]
Chemosensors 07 00001 g001 550
Figure 1. Schematic representation of the preparation of MnO2/unzipped SWCNTs modified glassy carbon electrode (GCE) for glucose sensing.
Figure 1. Schematic representation of the preparation of MnO2/unzipped SWCNTs modified glassy carbon electrode (GCE) for glucose sensing.
Chemosensors 07 00001 g001
Chemosensors 07 00001 g002 550
Figure 2. SEM images of pristine SWCNTs (A), unzipped SWCNTs (B), and spike like MnO2 on unzipped SWCNTs (C). TEM image of MnO2/unzipped SWCNTs (D).
Figure 2. SEM images of pristine SWCNTs (A), unzipped SWCNTs (B), and spike like MnO2 on unzipped SWCNTs (C). TEM image of MnO2/unzipped SWCNTs (D).
Chemosensors 07 00001 g002
Chemosensors 07 00001 g003 550
Figure 3. Comparisons of unzipped (---) and pristine (-) SWCNTs in (A) FTIR transmittance and (B) Raman spectra.
Figure 3. Comparisons of unzipped (---) and pristine (-) SWCNTs in (A) FTIR transmittance and (B) Raman spectra.
Chemosensors 07 00001 g003
Chemosensors 07 00001 g004 550
Figure 4. Cyclic voltammograms (oxidation) in 0.5 M H2SO4 at 100 mV s−1 scan rate in the potential window from −0.2 to 0.7 V vs. Ag/AgCl sat. KCl.
Figure 4. Cyclic voltammograms (oxidation) in 0.5 M H2SO4 at 100 mV s−1 scan rate in the potential window from −0.2 to 0.7 V vs. Ag/AgCl sat. KCl.
Chemosensors 07 00001 g004
Chemosensors 07 00001 g005 550
Figure 5. (A) Cyclic voltammograms (CVs) obtained at pristine SWCNTs and unzipped SWCNTs modified GC electrode in 0.1 M Na2SO4 containing 5.0 mM MnSO4, at scan rate of 100 mV−1; (B) electrodeposition of MnO2 by repetitive CVs on unzipped SWCNTs/GCE.
Figure 5. (A) Cyclic voltammograms (CVs) obtained at pristine SWCNTs and unzipped SWCNTs modified GC electrode in 0.1 M Na2SO4 containing 5.0 mM MnSO4, at scan rate of 100 mV−1; (B) electrodeposition of MnO2 by repetitive CVs on unzipped SWCNTs/GCE.
Chemosensors 07 00001 g005
Chemosensors 07 00001 g006 550
Figure 6. (A) Cyclic voltammograms (CVs) of different electrodes: (a) pristine SWCNTs, (b) only MnO2, (c) unzipped SWCNTs, and (d) MnO2/unzipped SWCNTs film modified GCE in presence of 1.0 mM of H2O2; (B) CVs of MnO2/unzipped SWCNTs with different concentration of H2O2 (1.0 mM to 5.0 mM) in 0.1 M PBS (pH 7.4) at scan rate 100 mV/s.
Figure 6. (A) Cyclic voltammograms (CVs) of different electrodes: (a) pristine SWCNTs, (b) only MnO2, (c) unzipped SWCNTs, and (d) MnO2/unzipped SWCNTs film modified GCE in presence of 1.0 mM of H2O2; (B) CVs of MnO2/unzipped SWCNTs with different concentration of H2O2 (1.0 mM to 5.0 mM) in 0.1 M PBS (pH 7.4) at scan rate 100 mV/s.
Chemosensors 07 00001 g006
Chemosensors 07 00001 g007 550
Figure 7. Current–time curve obtained on MnO2/unzipped SWCNTs/GCE with successive addition of different concentration of H2O2 to 0.1 M, pH 7.4, PBS solution; inset: Calibration illustrating the linear relationship between electrode response to the concentration of H2O2.
Figure 7. Current–time curve obtained on MnO2/unzipped SWCNTs/GCE with successive addition of different concentration of H2O2 to 0.1 M, pH 7.4, PBS solution; inset: Calibration illustrating the linear relationship between electrode response to the concentration of H2O2.
Chemosensors 07 00001 g007
Chemosensors 07 00001 g008 550
Figure 8. Current–time curve of MnO2/unzipped SWCNTs/GCE with successive addition of glucose to 0.1 M, pH 7.4, PBS solution containing 1 mg/mL GOx at 0.85 V (vs. Ag/AgCl). Inset: Calibration illustrating the linear relationship between electrode responses to the glucose addition.
Figure 8. Current–time curve of MnO2/unzipped SWCNTs/GCE with successive addition of glucose to 0.1 M, pH 7.4, PBS solution containing 1 mg/mL GOx at 0.85 V (vs. Ag/AgCl). Inset: Calibration illustrating the linear relationship between electrode responses to the glucose addition.
Chemosensors 07 00001 g008
Chemosensors 07 00001 g009 550
Figure 9. (A) Current–time curve of MnO2/Unzipped SWCNTs/GCE electrode in absence of glucose over 3000 s and (B) the sequential addition of 0.1 mM glucose, 0.1 mM UA and 0.1 mM AA 0.1 M, pH 7.4, PBS solution containing 1 mg/mL GOx at 0.85 V (vs. Ag/AgCl).
Figure 9. (A) Current–time curve of MnO2/Unzipped SWCNTs/GCE electrode in absence of glucose over 3000 s and (B) the sequential addition of 0.1 mM glucose, 0.1 mM UA and 0.1 mM AA 0.1 M, pH 7.4, PBS solution containing 1 mg/mL GOx at 0.85 V (vs. Ag/AgCl).
Chemosensors 07 00001 g009
Table
Table 1. Comparative performance of different composite electrodes for H2O2 detection.
Table 1. Comparative performance of different composite electrodes for H2O2 detection.
Modified ElectrodeLinear Range (mM)Detection Limit (µM)Reference
Pt NP/graphite-CS0.25–2890 µM66 nM[35]
rGO/FeNPs/GCE0.001–2.150.056[28]
AgNPs/Na2Ti3O7/Graphite5.0–25001.0[29]
MnO2/CuO/GO NP/Cu wire0.5–4.453.0[38]
MnO2/SWCNTs-Nf/GCE0.005–3.00.52Our previous work [27]
MnO2/unzipped SWCNTs/GCE0.002–5.00.3This work
Table
Table 2. Comparative performance of different composite electrodes for glucose detection.
Table 2. Comparative performance of different composite electrodes for glucose detection.
Modified ElectrodeLinear Range (mM)Detection Limit (µM)Reference
Mn3O4 NP/3D GF0.1–810[39]
SnO2 NP/rGO/GCE0.05–5.0013.35[40]
Mn-Cu NP/MWCNT/GCE1–321.0[41]
MnO2/CuO/GO NP/Cu wire0.5–4.453[38]
GOx/rGO/CD/GCE0.050–3.012[31]
MnO2/unzipped SWCNTs/GC0.01–1.21.2This work

Share and Cite

MDPI and ACS Style

Zakaria, A.B.M.; Leszczynska, D. Electrochemically Prepared Unzipped Single Walled Carbon Nanotubes-MnO2 Nanostructure Composites for Hydrogen Peroxide and Glucose Sensing. Chemosensors 2019, 7, 1. https://doi.org/10.3390/chemosensors7010001

AMA Style

Zakaria ABM, Leszczynska D. Electrochemically Prepared Unzipped Single Walled Carbon Nanotubes-MnO2 Nanostructure Composites for Hydrogen Peroxide and Glucose Sensing. Chemosensors. 2019; 7(1):1. https://doi.org/10.3390/chemosensors7010001

Chicago/Turabian Style

Zakaria, A. B. M., and Danuta Leszczynska. 2019. "Electrochemically Prepared Unzipped Single Walled Carbon Nanotubes-MnO2 Nanostructure Composites for Hydrogen Peroxide and Glucose Sensing" Chemosensors 7, no. 1: 1. https://doi.org/10.3390/chemosensors7010001

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop