Electrochemically Prepared Unzipped Single Walled Carbon Nanotubes-MnO 2 Nanostructure Composites for Hydrogen Peroxide and Glucose Sensing

Amperometric hydrogen peroxide (H2O2) and glucose biosensors based on unzipped carbon nanotubes with modified glassy carbon electrode (GCE) have been successfully fabricated via a facile electrochemical oxidative method. In this work, we investigated the feasibility of this new form of carbon nanomaterial as a substrate electrode material for fabricating sensitive platform for H2O2 and glucose sensors. For this purpose, the manganese oxide (MnO2)/unzipped single-walled carbon nanotubes (SWCNTs) film was synthesized by the cyclic voltammetry method. The developed sensing film, MnO2/unzipped SWCNTs/GCE, displayed a satisfactory analytical performance for H2O2, including a wide linear range of 2.0 × 10−6 to 5.0 × 10−3 M with a detection limit of 0.31 × 10−6 M (10.7 ppb). This film was further applied for glucose sensing with a linearity range of 0.01 to 1.2 mM with a correlation coefficient of 0.9822 in the physiological pH (7.4). This facile, fast, environmentally-friendly, and economical preparation strategy of carbon nanomaterial-based electrode materials opens up the possibility of developing high quality biocompatible hydrogen peroxide and glucose sensors.


Introduction
Unzipped carbon nanotubes, a new transformed material commonly synthesized from carbon nanotubes (CNTs), are a combination of one-dimensional CNTs and two-dimensional graphene [1].This unique nanostructure has also been called graphene nanoribbons (GNRs) [2,3].It has attracted enormous interest from both theoretical and experimental scientists due to its unique electrical [4], optical [5], thermal [6], doping [7], magnetic [8], and mechanical [9] properties.To date, numerous experiments have been performed to categorize the practical significance in manufacturing devices, such as field-effect transistors [10], photodetectors [11], catalysts for proton exchange membrane (PEM) fuel cells [12], and magnetic field sensors [13].However, very few reports have been published on using this carbon nanostructure as a substrate material for constructing sensors for specific measurements, such as hydrogen peroxide and glucose.
Various preparation methods for unzipping carbon nanotubes are known, such as plasma etching [14], annealing in hydrogen [15], intercalation-exfoliation [16], catalytic metal nanoparticle cutting [17], and chemical unzipping [18,19].Among these methods, the electrochemical approach is an effective way for converting carbon nanotubes (CNTs) to unzipped carbon nanotubes.Moreover, the electrochemical method has a unique advantage when compared with other methods, as it allows controlling of the layer's thickness and orientation [20][21][22].Graphene-like unzipped carbon nanotubes consist of a sp 2 carbon plane, but have an elongated structure with a high aspect ratio, edge density, and defects sites, as well as high-quality graphene layers.These unique properties make them an excellent candidate for electrochemical sensing platforms with high current responses, and lower limits of detection towards target analytes.
Novel metal nanostructures incorporating carbon nanomaterials (CNMs) have shown great potential applications in the fields of electrochemical sensing devices.Recently, metal and transition metal oxide nanoparticles have been extensively applied in developing high efficient electrochemical sensing toward H 2 O 2 and glucose [23][24][25].Particularly, manganese oxide (MnO 2 ) nanoparticles are one of the most promising inorganic materials in fabricating sensing devices due to high catalytic ability, low-cost, high energy density, and environmental benignity [26][27][28][29].
Nowadays, H 2 O 2 and glucose are suitable probe analytes to justify a new material in future biosensing applications.Several new forms of hybrid materials have been investigated by using these probe analytes.Most importantly, CNM-based sensors possess fast electron transfer kinetics, low limits of detection, and high sensitivity.For example, Yaojuan et al. investigated gold nanoparticle (AuNP)-graphene nanocomposites as a new electrode material synthesized by electrochemical deposition of Au-NPs on graphene sheets [30].Selvakumar et al. demonstrated the glucose biosensing properties of a direct growth of glucose oxidase (GOx) on reduced graphene oxide/β-Cyclodextrin composite modified electrode for glucose monitoring [31].Qinglin et al. fabricated glassy carbon electrode with graphene-carbon nanotube-nafion/gold-platinum alloy nanoparticles [32].Nur et al. used Au nanoparticles supported graphene oxide nanoribbons (GONRs) [33].Minmin et al. demonstrated Cu 2 O nanocubes wrapped in graphene nanosheets (Cu 2 O/GNs) as electrocatalysts [34].Jasmina et al. reported an amperometric H 2 O 2 sensor using a carbon paste electrode (CPE) coated with platinum nanoparticles on graphitized carbon (Pt-C) [35].
The preparation of these electrode materials is usually time-consuming and high-cost, and requires complicated, multi-step procedures.Therefore, a simple, low-cost preparation method to fabricate electrode is highly desired.In our previous work, we have achieved substantial progress on preparing substrate electrode material including synthesis and characterization of unzipped single-walled carbon nanotubes (unzipped SWCNTs) [36].The obtained new form of carbon nanomaterial offers high active sites, fast electron transfer kinetics, and effective high surface area.
In this work, we report our extended study on preparing the final electrode material by the electrodeposition of MnO 2 on as-prepared unzipped SWCNTs modified glassy carbon electrode (GCE) and evaluating its electrocatalytic performances towards electrooxidation of the probe analytes, such as H 2 O 2 and glucose.
Amperometric and cyclic voltammograms were carried out with CH Instruments (CHI 440, CH Instruments, Austin, TX, USA) using a standard three-electrode system.The glassy carbon electrode (GCE), Ag/AgCl/saturated KCl (Model CH111, CH Instruments, Austin, TX, USA), and a platinum wire were used as working, reference, and counter electrodes, respectively.High purity argon was used to deoxygenate all experimental solutions for 20 min, and maintaining argon atmosphere during experiments.Scanning electron microscopy (ZEISS Supra 40VP SEM) and transmission electron microscopy (JEOL-2100F operated at 100 kV) were used for characterizing the surface morphology and nanostructure of the carbon nanomaterials and MnO 2 nanoparticles.

Preparation of MnO 2 /Unzipped SWCNTs/GCE
Unzipped SWCNTs/GCE was prepared as reported before [20,36].Briefly, 5 mg of SWCNTs was dispersed in 5 mL of Nafion under 20 min constant ultrasonic agitations until obtaining 1 mg/mL of black homogeneous suspension.The glassy carbon electrode was polished with 0.05 µm alumina powder, and washed ultrasonically in deionized water and ethanol for 10 min.Then, a 3 µL of the black suspension was dropped onto cleaned GCE surface, and dried under a yellow lamp at 50 • C to obtain the SWCNTs/GCE.
The modified GCE was subjected to electrochemical oxidation in potential window of −0.2 to 0.7 V, at scan rate 100 mVs −1 in 0.5 M H 2 SO 4 for 12 h.Then, MnO 2 was electrodeposited on the surface of unzipped SWCNTs/GCE by repetitive scanning cycles by applying potential window of 0.3 to 1.3 V in a solution of 0.1 M Na 2 SO 4 and 5.0 mM MnSO 4 .The electrochemical setup, including the steps of preparation method of modified glassy carbon electrode (GCE) and electrochemical reactions in sensing H 2 O 2 and glucose, is illustrated in Figure 1.
Chemosensors 2019, 7, x FOR PEER REVIEW 3 of 12 used to deoxygenate all experimental solutions for 20 min, and maintaining argon atmosphere during experiments.Scanning electron microscopy (ZEISS Supra 40VP SEM) and transmission electron microscopy (JEOL-2100F operated at 100 kV) were used for characterizing the surface morphology and nanostructure of the carbon nanomaterials and MnO2 nanoparticles.

Preparation of MnO2/Unzipped SWCNTs/GCE
Unzipped SWCNTs/GCE was prepared as reported before [20,36].Briefly, 5 mg of SWCNTs was dispersed in 5 mL of Nafion under 20 min constant ultrasonic agitations until obtaining 1 mg/mL of black homogeneous suspension.The glassy carbon electrode was polished with 0.05 µm alumina powder, and washed ultrasonically in deionized water and ethanol for 10 min.Then, a 3 µL of the black suspension was dropped onto cleaned GCE surface, and dried under a yellow lamp at 50 °C to obtain the SWCNTs/GCE.
The modified GCE was subjected to electrochemical oxidation in potential window of −0.2 to 0.7 V, at scan rate 100 mVs −1 in 0.5 M H2SO4 for 12 h.Then, MnO2 was electrodeposited on the surface of unzipped SWCNTs/GCE by repetitive scanning cycles by applying potential window of 0.3 to 1.3 V in a solution of 0.1 M Na2SO4 and 5.0 mM MnSO4.The electrochemical setup, including the steps of preparation method of modified glassy carbon electrode (GCE) and electrochemical reactions in sensing H2O2 and glucose, is illustrated in Figure 1.

Characterization of Unzipped SWCNTs and MnO2 Nanostructured Film
The surface of SWCNTs was investigated before and after electrochemical treatment by scanning electron microscopy (SEM).Figure 2A shows the SEM image of the characteristic tubular networks of pristine SWCNTs.After anodic oxidation, a cloudy surface morphology of CNT was observed (Figure 2B).This cloudy feature of unzipped SWCNTs was observed due to increasing

Characterization of Unzipped SWCNTs and MnO 2 Nanostructured Film
The surface of SWCNTs was investigated before and after electrochemical treatment by scanning electron microscopy (SEM).Figure 2A shows the SEM image of the characteristic tubular networks of pristine SWCNTs.After anodic oxidation, a cloudy surface morphology of CNT was observed (Figure 2B).This cloudy feature of unzipped SWCNTs was observed due to increasing edge carbon resulting hydrophilic nature developing on its surface and attaching atmospheric water molecules.In addition, Figure 2C, 2D depicts the spike-like MnO2 anchored with edge carbon generated during transformation of SWCNTs to unzipped SWCNTs.The homogeneous structure of MnO2/unzipped SWCNTs film suggests that MnO2 was successfully attached on the surface of unzipped SWCNTs.
In order to confirm the oxidative opening (unzipping) of SWCNTs, we carried out Fourier transform infrared spectroscopy (FTIR) and Raman spectroscopy analyses.The FTIR (Figure 3A) spectrum of unzipped SWCNTs illustrates higher absorption of O-H group in the regions of O-H (3000-3700 cm −1 ) and C=O modes (1700-1750 cm −1 ), and lower absorption in the regions of C-O (1000-1250 cm −1 ) and C=C-H (800-1000 cm −1 ) compared to the spectrum of pristine SWCNTs.Moreover, Raman spectra (Figure 3B) also confirmed the fully unzipping of pristine SWCNTs.The G and D bands of SWCNTs has been reported as the characteristics of sp 2 carbon atoms and the extent of defects of carbon nanostructure respectively [37].The spectrum of unzipped SWCNTs in Figure 3B shows the intensity of the D band at 1360 cm −1 and the G band at 1600 cm −1 s increased and broadened compared to the pristine SWCNTs.This observation is attributed to the confirmation of the formation of unzipped SWCNTs.In addition, Figure 2C,D depicts the spike-like MnO 2 anchored with edge carbon generated during transformation of SWCNTs to unzipped SWCNTs.The homogeneous structure of MnO 2 /unzipped SWCNTs film suggests that MnO 2 was successfully attached on the surface of unzipped SWCNTs.
In order to confirm the oxidative opening (unzipping) of SWCNTs, we carried out Fourier transform infrared spectroscopy (FTIR) and Raman spectroscopy analyses.The FTIR (Figure 3A) spectrum of unzipped SWCNTs illustrates higher absorption of O-H group in the regions of O-H (3000-3700 cm −1 ) and C=O modes (1700-1750 cm −1 ), and lower absorption in the regions of C-O (1000-1250 cm −1 ) and C=C-H (800-1000 cm −1 ) compared to the spectrum of pristine SWCNTs.Moreover, Raman spectra (Figure 3B) also confirmed the fully unzipping of pristine SWCNTs.The G and D bands of SWCNTs has been reported as the characteristics of sp 2 carbon atoms and the extent of defects of carbon nanostructure respectively [37].The spectrum of unzipped SWCNTs in Figure 3B shows the intensity of the D band at 1360 cm −1 and the G band at 1600 cm −1 s increased and broadened compared to the pristine SWCNTs.This observation is attributed to the confirmation of the formation of unzipped SWCNTs.Figure 5A shows the cyclic voltammograms (CVs) obtained in solution of 0.1M Na2SO4 and 5.0 mM MnSO4 at SWCNTs/GCE and unzipped SWCNTs/GCE.Obtained data strongly demonstrated that the sharp and large peak area of oxidizing Mn 2+ to Mn 3+ and reducing Mn 3+ to Mn 2+ was observed at unzipping SWCNTs when compared to pristine SWCNTs.The current intensity of oxidation and reduction during electrodeposition of MnO2 was increased with repetitive voltammetric cycles (Figure 5B).This observation is consistent with our previous work [27].It also suggests that large amounts of MnO2 nanoparticles were successfully attached due to enlarged surface area with increasing active sites on unzipped SWCNTs.

Formation of MnO2 on Unzipped SWCNTs/GCE
Figure 4 shows the voltammograms of the first scan and scans after 12 h and 72 h, respectively, indicating the stable and successful unzipping of carbon nanotubes.Figure 5A shows the cyclic voltammograms (CVs) obtained in solution of 0.1M Na2SO4 and 5.0 mM MnSO4 at SWCNTs/GCE and unzipped SWCNTs/GCE.Obtained data strongly demonstrated that the sharp and large peak area of oxidizing Mn 2+ to Mn 3+ and reducing Mn 3+ to Mn 2+ was observed at unzipping SWCNTs when compared to pristine SWCNTs.The current intensity of oxidation and reduction during electrodeposition of MnO2 was increased with repetitive voltammetric cycles (Figure 5B).This observation is consistent with our previous work [27].It also suggests that large amounts of MnO2 nanoparticles were successfully attached due to enlarged surface area with increasing active sites on unzipped SWCNTs.Figure 5A shows the cyclic voltammograms (CVs) obtained in solution of 0.1M Na 2 SO 4 and 5.0 mM MnSO 4 at SWCNTs/GCE and unzipped SWCNTs/GCE.Obtained data strongly demonstrated that the sharp and large peak area of oxidizing Mn 2+ to Mn 3+ and reducing Mn 3+ to Mn 2+ was observed at unzipping SWCNTs when compared to pristine SWCNTs.The current intensity of oxidation and reduction during electrodeposition of MnO 2 was increased with repetitive voltammetric cycles (Figure 5B).This observation is consistent with our previous work [27].It also suggests that large amounts of MnO 2 nanoparticles were successfully attached due to enlarged surface area with increasing active sites on unzipped SWCNTs.
that the sharp and large peak area of oxidizing Mn 2+ to Mn 3+ and reducing Mn 3+ to Mn 2+ was observed at unzipping SWCNTs when compared to pristine SWCNTs.The current intensity of oxidation and reduction during electrodeposition of MnO2 was increased with repetitive voltammetric cycles (Figure 5B).This observation is consistent with our previous work [27].It also suggests that large amounts of MnO2 nanoparticles were successfully attached due to enlarged surface area with increasing active sites on unzipped SWCNTs.The pristine SWCNTs did not show any oxidation peak indicating its electrochemical inactivity in this potential window.The unzipped SWCNTs modified GCE showed some oxidation current due to available active sites generated during the opening of the side wall of the carbon nanotubes.In contrast, a noticeable oxidation peak at the potential of 0.85 V was observed at MnO2/unzipped SWCNTs/GCE resulting from two factors.Firstly, MnO2 has high catalytic activity for H2O2.Secondly, spike or edge-like structure of MnO2 nanoparticles electrodeposited on unzipped SWCNTs might have higher catalytic ability for decomposing H2O2 than regular-sized nanoparticles.From Figure 6B, it is obvious that the oxidation peak current was proportional to increasing H2O2 concentration (a-f: 1.0 mM to 5.0 mM).The excellent electrochemical activity toward H2O2 can be attributed to the relatively high specific surface area, and the orientation of nanostructured MnO2 on the unzipped SWCNTs/GCE surface.Secondly, spike or edge-like structure of MnO 2 nanoparticles electrodeposited on unzipped SWCNTs might have higher catalytic ability for decomposing H 2 O 2 than regular-sized nanoparticles.From Figure 6B, it is obvious that the oxidation peak current was proportional to increasing H 2 O 2 concentration (a-f: 1.0 mM to 5.0 mM).The excellent electrochemical activity toward H 2 O 2 can be attributed to the relatively high specific surface area, and the orientation of nanostructured MnO 2 on the unzipped SWCNTs/GCE surface.

Determination of H 2 O 2
where x is the concentration of H 2 O 2 in µM.
The linear range of this calibration curve was from 2.0 × 10 −6 to 5.0 × 10 −3 M with a correlation coefficient of 0.9831.From the slope of 0.0209 µA/µM, the limit of detection (LOD) was calculated to 0.31 × 10 −6 M using the following equation: where S is slop or sensitivity, SD: Standard deviation.The linearity range and sensitivity for detecting H 2 O 2 at MnO 2 /unzipped SWCNTs/GCE was greater than that of MnO 2 /pristine SWCNTs/GCE, as was reported in our previous work [30].In addition, the sensor exhibited appreciable reproducibility of 3.78% for five independent measurements carried out by five different MnO 2 /unzipped SWCNT film modified electrodes.The linearity range and sensitivity for detecting H2O2 at MnO2/unzipped SWCNTs/GCE was greater than that of MnO2/pristine SWCNTs/GCE, as was reported in our previous work [30].In addition, the sensor exhibited appreciable reproducibility of 3.78% for five independent measurements carried out by five different MnO2/unzipped SWCNT film modified electrodes.Table 1 summarizes performance of different modified electrodes toward direct measurements of hydrogen peroxide in water matrix.Glucose oxidase (GOx) was taken as the representative enzyme to investigate the electrocatalytic ability of MnO 2 /unzipped SWCNTs modified GCE during glucose monitoring.As is well-known, glucose oxidase catalyzes the oxidation of glucose with oxygen, accompanying the production of gluconic acid and H 2 O 2 .Consequently, the concentration of glucose can be indirectly detected by the determination of the liberated H 2 O 2 during the enzymatic catalysis.Figure 8 shows the amperometric response of the MnO 2 /unzipped SWCNTs/GCE during successive addition of glucose to a solution containing 1 mg/1 mL GOx.Based on the current response towards the oxidation of H 2 O 2 during the enzyme/glucose reaction, the calibration curve was plotted between catalytic current and glucose concentration.The linearity range was estimated from 0.01 × 10 −3 to 1.2 × 10 −3 M with a correlation coefficient of 0.9822 and the detection limit of the glucose was 1.2 × 10 −6 M. Table 2 summarizes performance of several composite electrodes toward glucose detection, including our work.The prepared electrode showed higher stability for amperometric measurement at a constant  The prepared electrode showed higher stability for amperometric measurement at a constant Figure 9A displays the stability response without addition of glucose to 0.1 M, pH 7.4, PBS solution containing 1 mg/mL GOx at 0.85 V (vs.Ag/AgCl).The reproducibility of the as-prepared electrode was also evaluated by measuring amperometric (E ap = 0.85 V) responses of the sequential addition of 0.1 mM of glucose in 0.1M PBS.The relative standard deviation (R.S.D) was 5.4% suggesting the high reproducibility of the prepared electrode.Moreover, Figure 9B shows the amperometric response of the sequential addition of 0.1 mM glucose, and the common interferents including uric acid (UA) and ascorbic acid (AA) of 0.1 mM for each addition.The significant response for glucose and insignificant responses for interfering species were observed, suggesting good selectivity for glucose detection.

Conclusions
We have outlined a facile methodology for constructing a suitable electrochemical sensing platform based on MnO2/unzipped SWCNTs that exhibits high electrocatalytic activity toward the oxidation of H2O2 in physiological pH of 7.4.Electrochemical method presents an efficient approach for tunable, simple and fast preparation of MnO2/unzipped SWCNTs film.More importantly, electrochemically synthesized unzipped SWCNTs provided sufficient anchoring sites that allowed the formation of high electrocatalytic MnO2 nanostructured film.Consequently, the as-prepared MnO2/unzipped SWCNTs can be used for monitoring H2O2 at a low potential of 0.85 V with high sensitivity.The estimated linearity range for measuring H2O2 was found to be from 2.0 × 10 −6 to 5.0 × 10 −3 M and the detection limit was 0.

Conclusions
We have outlined a facile methodology for constructing a suitable electrochemical sensing platform based on MnO 2 /unzipped SWCNTs that exhibits high electrocatalytic activity toward the oxidation of H 2 O 2 in physiological pH of 7.4.Electrochemical method presents an efficient approach for tunable, simple and fast preparation of MnO 2 /unzipped SWCNTs film.More importantly, electrochemically synthesized unzipped SWCNTs provided sufficient anchoring sites that allowed the formation of high electrocatalytic MnO 2 nanostructured film.Consequently, the as-prepared MnO 2 /unzipped SWCNTs can be used for monitoring H 2 O 2 at a low potential of 0.85 V with high sensitivity.The estimated linearity range for measuring H 2 O 2 was found to be from 2.0 × 10 −6 to 5.0 × 10 −3 M and the detection limit was 0.31 × 10 −6 M. Furthermore, this modified electrode was detecting very low concentration (1.2 × 10 −6 M) of glucose.The results show that MnO 2 /unzipped

Figure 1 .
Figure 1.Schematic representation of the preparation of MnO2/unzipped SWCNTs modified glassy carbon electrode (GCE) for glucose sensing.

Figure 1 .
Figure 1.Schematic representation of the preparation of MnO 2 /unzipped SWCNTs modified glassy carbon electrode (GCE) for glucose sensing.

Chemosensors 2019, 7 ,
x FOR PEER REVIEW 4 of 12 edge carbon resulting hydrophilic nature developing on its surface and attaching atmospheric water molecules.

Figure 4
Figure4shows the voltammograms of the first scan and scans after 12 h and 72 h, respectively, indicating the stable and successful unzipping of carbon nanotubes.

3. 2 .
Figure4shows the voltammograms of the first scan and scans after 12 h and 72 h, respectively, indicating the stable and successful unzipping of carbon nanotubes.

3. 3 . 12 Figure 5 .
Figure 6A displays the cyclic voltammetry (CV) obtained at the pristine SWCNTs/GCE (a), only MnO 2 /GCE (b), unzipped SWCNTs/GCE (c), and MnO 2 /unzipped SWCNTs/GCE (d) in 0.1 M PBS (pH 7.4) containing 1.0 mM H 2 O 2 , at a scan rate of 100 mVs −1 .The observed electrocatalytic ability of these different electrodes towards oxidation of H 2 O 2 has shown following order: MnO 2 /unzipped SWCNTs/GCE > unzipped SWCNTs/GCE > MnO 2 /GCE > Mn pristine SWCNTs/GCE.The pristine SWCNTs did not show any oxidation peak indicating its electrochemical inactivity in this potential window.The unzipped SWCNTs modified GCE showed some oxidation current due to available active sites generated during the opening of the side wall of the carbon nanotubes.In contrast, a noticeable oxidation peak at the potential of 0.85 V was observed at MnO 2 /unzipped SWCNTs/GCE resulting from two factors.Firstly, MnO 2 has high catalytic activity for H 2 O 2 .

Figure 7
Figure 7 displays the amperometric current-time curve of MnO2/unzipped SWCNTs/GCE for the successive addition of H2O2 in 0.1 M PBS (pH 7.4) at the potential +0.85 V.The figure (inset) also shows the calibration curve, and the regression equation of the sensor.The regression equation is as follows:Ip(μA) = 0.0209x + 1.7781(1)

Figure 7
Figure 7 displays the amperometric current-time curve of MnO 2 /unzipped SWCNTs/GCE for the successive addition of H 2 O 2 in 0.1 M PBS (pH 7.4) at the potential +0.85 V.The figure (inset) also shows the calibration curve, and the regression equation of the sensor.The regression equation is as follows:

Figure 7 .
Figure 7. Current-time curve obtained on MnO2/unzipped SWCNTs/GCE with successive addition of different concentration of H2O2 to 0.1 M, pH 7.4, PBS solution; inset: Calibration illustrating the linear relationship between electrode response to the concentration of H2O2.

Figure 7 .
Figure 7. Current-time curve obtained on MnO 2 /unzipped SWCNTs/GCE with successive addition of different concentration of H 2 O 2 to 0.1 M, pH 7.4, PBS solution; inset: Calibration illustrating the linear relationship between electrode response to the concentration of H 2 O 2 .

Figure 8 .
Figure 8. Current-time curve of MnO2/unzipped SWCNTs/GCE with successive addition of glucose to 0.1 M, pH 7.4, PBS solution containing 1 mg/mL GOx at 0.85 V (vs.Ag/AgCl).Inset: Calibration illustrating the linear relationship between electrode responses to the glucose addition.

Figure 8 .
Figure 8. Current-time curve of MnO 2 /unzipped SWCNTs/GCE with successive addition of glucose to 0.1 M, pH 7.4, PBS solution containing 1 mg/mL GOx at 0.85 V (vs.Ag/AgCl).Inset: Calibration illustrating the linear relationship between electrode responses to the glucose addition.

Table 1
summarizes performance of different modified electrodes toward direct measurements of hydrogen peroxide in water matrix.

Table 1 .
Comparative performance of different composite electrodes for H2O2 detection.

Table 1 .
Comparative performance of different composite electrodes for H 2 O 2 detection.

Table 2 .
Comparative performance of different composite electrodes for glucose detection.

Table 2 .
Comparative performance of different composite electrodes for glucose detection.