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Chemosensors
Volume 12
Issue 10
10.3390/chemosensors12100202
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Article

Application of a Novel Disposable Flow Cell for Spectroscopic Bioprocess Monitoring

by
Tobias Steinwedel
1,
Philipp Raithel
2,
Jana Schellenberg
1,
Carlotta Kortmann
1,
Pia Gellermann
1,
Mathias Belz
2 and
Dörte Solle
1,*
1
Institute of Technical Chemistry, Leibniz University, 30167 Hanover, Germany
2
LyteGate GmbH, 61169 Friedberg, Germany
*
Author to whom correspondence should be addressed.
Chemosensors 2024, 12(10), 202; https://doi.org/10.3390/chemosensors12100202
Submission received: 9 August 2024 / Revised: 27 September 2024 / Accepted: 29 September 2024 / Published: 1 October 2024
(This article belongs to the Special Issue Low-Cost Chemosensors for Applications in Environment, Health, Food, and Industry Process Control)
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Abstract

The evaluation of the analytical capabilities of a novel disposable flow cell for spectroscopic bioprocess monitoring is presented. The flow cell is presterilized and can be connected to any kind of bioreactor by weldable tube connections. It is clamped into a reusable holder, which is equipped with SMA-terminated optical fibers or an integrated light source and detection unit. This modular construction enables spectroscopic techniques like UV-Vis spectroscopy or turbidity measurements by scattered light for modern disposable bioreactors. A NIR scattering module was used for biomass monitoring in different cultivations. A high-cell-density fed-batch cultivation with Komagataella phaffii and a continuous perfusion cultivation with a CHO DG44 cell line were conducted. A high correlation between the sensor signal and biomass or viable cell count was observed. Furthermore, the sensor shows high sensitivity during low turbidity states, as well as a high dynamic range to monitor high turbidity values without saturation effects. In addition to upstream processing, the sensor system was used to monitor the purification process of a monoclonal antibody. The absorption module enables simple and cost-efficient monitoring of downstream processing and quality control measurements. Recorded absorption spectra can be used for antibody aggregate detection, due to an increase in overall optical density.
Keywords: bioprocess monitoring; online spectroscopy; disposable bioreactors bioprocess monitoring; online spectroscopy; disposable bioreactors

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MDPI and ACS Style

Steinwedel, T.; Raithel, P.; Schellenberg, J.; Kortmann, C.; Gellermann, P.; Belz, M.; Solle, D. Application of a Novel Disposable Flow Cell for Spectroscopic Bioprocess Monitoring. Chemosensors 2024, 12, 202. https://doi.org/10.3390/chemosensors12100202

AMA Style

Steinwedel T, Raithel P, Schellenberg J, Kortmann C, Gellermann P, Belz M, Solle D. Application of a Novel Disposable Flow Cell for Spectroscopic Bioprocess Monitoring. Chemosensors. 2024; 12(10):202. https://doi.org/10.3390/chemosensors12100202

Chicago/Turabian Style

Steinwedel, Tobias, Philipp Raithel, Jana Schellenberg, Carlotta Kortmann, Pia Gellermann, Mathias Belz, and Dörte Solle. 2024. "Application of a Novel Disposable Flow Cell for Spectroscopic Bioprocess Monitoring" Chemosensors 12, no. 10: 202. https://doi.org/10.3390/chemosensors12100202

APA Style

Steinwedel, T., Raithel, P., Schellenberg, J., Kortmann, C., Gellermann, P., Belz, M., & Solle, D. (2024). Application of a Novel Disposable Flow Cell for Spectroscopic Bioprocess Monitoring. Chemosensors, 12(10), 202. https://doi.org/10.3390/chemosensors12100202

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