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Open AccessReview

What Room for Two-Dimensional Gel-Based Proteomics in a Shotgun Proteomics World?

1
Medizinisches Proteom-Center, Medical Faculty & Medical Proteome Analysis, Center for Proteindiagnostics (PRODI) Ruhr-University Bochum Gesundheitscampus, 4 44801 Bochum, Germany
2
CBM UMR CNRS5249, Université Grenoble Alpes, CEA, CNRS, 17 rue des Martyrs, CEDEX 9, 38054 Grenoble, France
3
Laboratory of Chemistry and Biology of Metals, UMR 5249, Université Grenoble Alpes, CNRS, 38054 Grenoble, France
*
Author to whom correspondence should be addressed.
Proteomes 2020, 8(3), 17; https://doi.org/10.3390/proteomes8030017
Received: 28 June 2020 / Revised: 2 August 2020 / Accepted: 4 August 2020 / Published: 6 August 2020
(This article belongs to the Special Issue Proteomics: Technologies and Their Applications)
Two-dimensional gel electrophoresis was instrumental in the birth of proteomics in the late 1980s. However, it is now often considered as an outdated technique for proteomics—a thing of the past. Although this opinion may be true for some biological questions, e.g., when analysis depth is of critical importance, for many others, two-dimensional gel electrophoresis-based proteomics still has a lot to offer. This is because of its robustness, its ability to separate proteoforms, and its easy interface with many powerful biochemistry techniques (including western blotting). This paper reviews where and why two-dimensional gel electrophoresis-based proteomics can still be profitably used. It emerges that, rather than being a thing of the past, two-dimensional gel electrophoresis-based proteomics is still highly valuable for many studies. Thus, its use cannot be dismissed on simple fashion arguments and, as usual, in science, the tree is to be judged by the fruit. View Full-Text
Keywords: two-dimensional gel electrophoresis; proteomics; post-translational modifications; proteoforms; clinical proteomics two-dimensional gel electrophoresis; proteomics; post-translational modifications; proteoforms; clinical proteomics
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MDPI and ACS Style

Marcus, K.; Lelong, C.; Rabilloud, T. What Room for Two-Dimensional Gel-Based Proteomics in a Shotgun Proteomics World? Proteomes 2020, 8, 17.

AMA Style

Marcus K, Lelong C, Rabilloud T. What Room for Two-Dimensional Gel-Based Proteomics in a Shotgun Proteomics World? Proteomes. 2020; 8(3):17.

Chicago/Turabian Style

Marcus, Katrin; Lelong, Cécile; Rabilloud, Thierry. 2020. "What Room for Two-Dimensional Gel-Based Proteomics in a Shotgun Proteomics World?" Proteomes 8, no. 3: 17.

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