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Open AccessFeature PaperArticle

Vesiculation of Red Blood Cells in the Blood Bank: A Multi-Omics Approach towards Identification of Causes and Consequences

1
Department of Biochemistry (286), Radboud University Medical Center, 6500 HB Nijmegen, The Netherlands
2
Department of Applied Sciences, Faculty of Life and Health Sciences, Northumbria University, Newcastle-Upon-Tyne NE1 8ST, UK
3
School of Biomedical Sciences, University of Plymouth, Plymouth PL4 8AA, UK
4
Institute for Medical Physics and Biophysics, Medical Faculty, University of Leipzig, 4107 Leipzig, Germany
5
Department of Ecological and Biological Sciences (DEB), University of Tuscia, 01100 Viterbo, Italy
*
Author to whom correspondence should be addressed.
Proteomes 2020, 8(2), 6; https://doi.org/10.3390/proteomes8020006
Received: 7 February 2020 / Revised: 26 March 2020 / Accepted: 28 March 2020 / Published: 31 March 2020
(This article belongs to the Special Issue Red Blood Cell Multi-Omics)
Microvesicle generation is an integral part of the aging process of red blood cells in vivo and in vitro. Extensive vesiculation impairs function and survival of red blood cells after transfusion, and microvesicles contribute to transfusion reactions. The triggers and mechanisms of microvesicle generation are largely unknown. In this study, we combined morphological, immunochemical, proteomic, lipidomic, and metabolomic analyses to obtain an integrated understanding of the mechanisms underlying microvesicle generation during the storage of red blood cell concentrates. Our data indicate that changes in membrane organization, triggered by altered protein conformation, constitute the main mechanism of vesiculation, and precede changes in lipid organization. The resulting selective accumulation of membrane components in microvesicles is accompanied by the recruitment of plasma proteins involved in inflammation and coagulation. Our data may serve as a basis for further dissection of the fundamental mechanisms of red blood cell aging and vesiculation, for identifying the cause-effect relationship between blood bank storage and transfusion complications, and for assessing the role of microvesicles in pathologies affecting red blood cells. View Full-Text
Keywords: red blood cell; vesicles; proteomics; lipidomics; metabolomics; storage; aging red blood cell; vesicles; proteomics; lipidomics; metabolomics; storage; aging
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MDPI and ACS Style

Freitas Leal, J.K.; Lasonder, E.; Sharma, V.; Schiller, J.; Fanelli, G.; Rinalducci, S.; Brock, R.; Bosman, G. Vesiculation of Red Blood Cells in the Blood Bank: A Multi-Omics Approach towards Identification of Causes and Consequences. Proteomes 2020, 8, 6. https://doi.org/10.3390/proteomes8020006

AMA Style

Freitas Leal JK, Lasonder E, Sharma V, Schiller J, Fanelli G, Rinalducci S, Brock R, Bosman G. Vesiculation of Red Blood Cells in the Blood Bank: A Multi-Omics Approach towards Identification of Causes and Consequences. Proteomes. 2020; 8(2):6. https://doi.org/10.3390/proteomes8020006

Chicago/Turabian Style

Freitas Leal, Joames K.; Lasonder, Edwin; Sharma, Vikram; Schiller, Jürgen; Fanelli, Giuseppina; Rinalducci, Sara; Brock, Roland; Bosman, Giel. 2020. "Vesiculation of Red Blood Cells in the Blood Bank: A Multi-Omics Approach towards Identification of Causes and Consequences" Proteomes 8, no. 2: 6. https://doi.org/10.3390/proteomes8020006

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