The Proteome of Tetrasphaera elongata is adapted to Changing Conditions in Wastewater Treatment Plants
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cultivation and Sampling
2.2. Metabolomics & Proteomics
3. Results and Discussion
3.1. Efficient Growth under Anoxic Conditation
3.2. Cost-Effective Use of Substrates
3.3. Minor Changes in the Proteomes Upon Shift to Anoxic Conditions
3.4. PAO-Metabolism in T. elongata
4. Concluding Remarks
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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OD600nm | Protein Concentration | |||
---|---|---|---|---|
E. coli | T. elongata | E. coli | T. elongata | |
Aerobic phase (t = 0 h) | 0.183 ± 0.003 | 0.174 ± 0.026 | 107.8 ± 0.8 µg/mL | 78.4 ± 3.1 µg/mL |
Anoxic phase (t = 3 h) | 0.214 ± 0.009 | 0.209 ± 0.032 | 114.0 ± 5.1 µg/mL | 88.7 ± 1.8 µg/mL |
Absolute increase | 0.031 ± 0.009 | 0.036 ± 0.015 | 6.2 ± 5.3 µg/µL | 10.3 ± 3.8 µg/µL |
Relative increase | 16.8 ± 4.8% | 20.7 ± 9.2% | 5.8 ± 5.0% | 13.2 ± 5.3% |
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Herbst, F.-A.; Dueholm, M.S.; Wimmer, R.; Nielsen, P.H. The Proteome of Tetrasphaera elongata is adapted to Changing Conditions in Wastewater Treatment Plants. Proteomes 2019, 7, 16. https://doi.org/10.3390/proteomes7020016
Herbst F-A, Dueholm MS, Wimmer R, Nielsen PH. The Proteome of Tetrasphaera elongata is adapted to Changing Conditions in Wastewater Treatment Plants. Proteomes. 2019; 7(2):16. https://doi.org/10.3390/proteomes7020016
Chicago/Turabian StyleHerbst, Florian-Alexander, Morten S. Dueholm, Reinhard Wimmer, and Per Halkjær Nielsen. 2019. "The Proteome of Tetrasphaera elongata is adapted to Changing Conditions in Wastewater Treatment Plants" Proteomes 7, no. 2: 16. https://doi.org/10.3390/proteomes7020016