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Open AccessArticle

More than a Toxin: Protein Inventory of Clostridium tetani Toxoid Vaccines

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Microbiology Division, Department of Biology, Friedrich-Alexander University Erlangen-Nuremberg, Staudtstr. 5, 91058 Erlangen, Germany
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Biochemistry Division, Department of Biology, Friedrich-Alexander University Erlangen-Nuremberg, Staudtstr. 5, 91058 Erlangen, Germany
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Author to whom correspondence should be addressed.
Proteomes 2019, 7(2), 15; https://doi.org/10.3390/proteomes7020015
Received: 2 April 2019 / Revised: 12 April 2019 / Accepted: 15 April 2019 / Published: 16 April 2019
(This article belongs to the Special Issue Microbial Proteomics II)
Clostridium tetani is the etiological agent of tetanus, a life-threatening bacterial infection. The most efficient protection strategy against tetanus is a vaccination with the C. tetani neurotoxin, which is inactivated by formaldehyde-crosslinking. Since we assumed that besides the tetanus toxin, other proteins of C. tetani may also be present in toxoid preparations, we analyzed commercially available vaccines from different countries in respect to their protein content using mass spectrometry. In total 991 proteins could be identified in all five analyzed vaccines, 206 proteins were common in all analyzed vaccines and 54 proteins from the 206 proteins were potential antigens. The additionally present proteins may contribute at least partially to protection against C. tetani infection by supporting the function of the vaccine against the devastating effects of the tetanus toxin indirectly. Two different label-free protein quantification methods were applied for an estimation of protein contents. Similar results were obtained with a Total Protein Approach (TPA)-based method and Protein Discoverer 2.2 software package based on the minora algorithm. Depending on the tetanus toxoid vaccine and the quantification method used, tetanus neurotoxin contributes between 14 and 76 % to the total C. tetani protein content and varying numbers of other C. tetani proteins were detected. View Full-Text
Keywords: Clostridium tetani; label-free quantification; neurotoxin; proteomics; tetanospasmin; vaccination Clostridium tetani; label-free quantification; neurotoxin; proteomics; tetanospasmin; vaccination
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MDPI and ACS Style

Möller, J.; Kraner, M.E.; Burkovski, A. More than a Toxin: Protein Inventory of Clostridium tetani Toxoid Vaccines. Proteomes 2019, 7, 15.

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