Next Article in Journal
Retrospective Proteomic Screening of 100 Breast Cancer Tissues
Previous Article in Journal
A Routine ‘Top-Down’ Approach to Analysis of the Human Serum Proteome
Previous Article in Special Issue
Effects of Al3+ and La3+ Trivalent Metal Ions on Tomato Fruit Proteomes
Open AccessFeature PaperArticle

Comparison between Proteome and Transcriptome Response in Potato (Solanum tuberosum L.) Leaves Following Potato Virus Y (PVY) Infection

Department of Biotechnology and Systems Biology, National Institute of Biology, 1000 Ljubljana, Slovenia
Department of Ecogenomics and Systems Biology, Faculty of Life Sciences, University of Vienna, 1010 Wien, Austria
Author to whom correspondence should be addressed.
Proteomes 2017, 5(3), 14;
Received: 4 May 2017 / Revised: 27 June 2017 / Accepted: 1 July 2017 / Published: 6 July 2017
(This article belongs to the Special Issue Proteomics in Plant–Environment Interactions)
Plant diseases caused by viral infection are affecting all major crops. Being an obligate intracellular organisms, chemical control of these pathogens is so far not applied in the field except to control the insect vectors of the viruses. Understanding of molecular responses of plant immunity is therefore economically important, guiding the enforcement of crop resistance. To disentangle complex regulatory mechanisms of the plant immune responses, understanding system as a whole is a must. However, integrating data from different molecular analysis (transcriptomics, proteomics, metabolomics, smallRNA regulation etc.) is not straightforward. We evaluated the response of potato (Solanum tuberosum L.) following the infection with potato virus Y (PVY). The response has been analyzed on two molecular levels, with microarray transcriptome analysis and mass spectroscopy-based proteomics. Within this report, we performed detailed analysis of the results on both levels and compared two different approaches for analysis of proteomic data (spectral count versus MaxQuant). To link the data on different molecular levels, each protein was mapped to the corresponding potato transcript according to StNIB paralogue grouping. Only 33% of the proteins mapped to microarray probes in a one-to-one relation and additionally many showed discordance in detected levels of proteins with corresponding transcripts. We discussed functional importance of true biological differences between both levels and showed that the reason for the discordance between transcript and protein abundance lies partly in complexity and structure of biological regulation of proteome and transcriptome and partly in technical issues contributing to it. View Full-Text
Keywords: potato virus Y (PVY); proteomics; transcriptomics; potato (Solanum tuberosum L.) potato virus Y (PVY); proteomics; transcriptomics; potato (Solanum tuberosum L.)
Show Figures

Figure 1

MDPI and ACS Style

Stare, T.; Stare, K.; Weckwerth, W.; Wienkoop, S.; Gruden, K. Comparison between Proteome and Transcriptome Response in Potato (Solanum tuberosum L.) Leaves Following Potato Virus Y (PVY) Infection. Proteomes 2017, 5, 14.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

Back to TopTop