Genome-Wide Identification, Characterization, and Regulation of RWP-RK Gene Family in the Nitrogen-Fixing Clade
Abstract
:1. Introduction
2. Results
2.1. Phylogeny of NFC Species and Distribution of RWP-RKs
2.2. Phylogeny and Characteristics of the NFC RWP-RKs
2.3. Comparison of RWP-RKs in Non-Nodulating (A. thaliana) vs. Nodulating Plants (G. max and P. vulgaris)
2.4. The Involved Biological Processes of AtNLPs-Associated Modules under N starvation Condition
2.5. Effects of High and Low Nitrogen on Nodulation via the Reglation of PvNLP Genes in P. vulgaris
3. Discussion
3.1. Distribution and Features of RWP-RKs in NFC
3.2. Effects of N Starvation on Nodulation Possibly Mediated by Regulation of NLP Genes
3.3. Simulation of Low Nitrogen on Nodulation Mediated by Differential Expression of PvNLP Genes in P. vulgaris
4. Materials and Methods
4.1. Inference of Species Tree Based on Gene Duplication Events
4.2. Identification and Characterization of RWP-RK Genes
4.3. Phylogenetic Analysis of RWP-RKs
4.4. Co-Expression Network Analysis
4.5. Plant Materials, Growth and Treatment Conditions
4.6. Quantitative RT-PCR
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Wu, Z.; Liu, H.; Huang, W.; Yi, L.; Qin, E.; Yang, T.; Wang, J.; Qin, R. Genome-Wide Identification, Characterization, and Regulation of RWP-RK Gene Family in the Nitrogen-Fixing Clade. Plants 2020, 9, 1178. https://doi.org/10.3390/plants9091178
Wu Z, Liu H, Huang W, Yi L, Qin E, Yang T, Wang J, Qin R. Genome-Wide Identification, Characterization, and Regulation of RWP-RK Gene Family in the Nitrogen-Fixing Clade. Plants. 2020; 9(9):1178. https://doi.org/10.3390/plants9091178
Chicago/Turabian StyleWu, Zhihua, Hong Liu, Wen Huang, Lisha Yi, Erdai Qin, Tiange Yang, Jing Wang, and Rui Qin. 2020. "Genome-Wide Identification, Characterization, and Regulation of RWP-RK Gene Family in the Nitrogen-Fixing Clade" Plants 9, no. 9: 1178. https://doi.org/10.3390/plants9091178