The Ethylene Biosynthesis Genes ACS2 and ACS6 Modulate Disease Severity of Verticillium dahliae
Abstract
:1. Introduction
2. Results
2.1. Verticillium Wilt Symptom Development
2.2. Verticillium dahliae DNA qPCR Quantification
2.3. ACS2 and ACS6 Expression upon Verticillium dahliae Plant Infection
2.4. Exogenous ACC and AOA Application to wt Arabidopsis thaliana before or after Verticillium dahliae Inoculation
3. Discussion
4. Materials and Methods
4.1. Fungal Culture
4.2. Seeds Origin and Plant Growth Conditions
4.3. Pathogenicity Experiments
4.4. DNA Extraction and qPCR Fungal Quantification
4.5. Determination of Transcript Levels Using RT-PCR Assay
4.6. Statistics
Author Contributions
Funding
Conflicts of Interest
References
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ACS2 | ACS6 | |||
---|---|---|---|---|
Source | df | F | df | F |
Tissue | 1 | 413.06 ** | 1 | 2.53 |
Time | 2 | 90.09 ** | 2 | 118.14 ** |
Tissue × Time | 2 | 7.29 * | 2 | 46.80 ** |
Error | 48 | 48 | ||
Total | 54 | 54 |
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Poulaki, E.G.; Tsolakidou, M.-D.; Gkizi, D.; Pantelides, I.S.; Tjamos, S.E. The Ethylene Biosynthesis Genes ACS2 and ACS6 Modulate Disease Severity of Verticillium dahliae. Plants 2020, 9, 907. https://doi.org/10.3390/plants9070907
Poulaki EG, Tsolakidou M-D, Gkizi D, Pantelides IS, Tjamos SE. The Ethylene Biosynthesis Genes ACS2 and ACS6 Modulate Disease Severity of Verticillium dahliae. Plants. 2020; 9(7):907. https://doi.org/10.3390/plants9070907
Chicago/Turabian StylePoulaki, Eirini G., Maria-Dimitra Tsolakidou, Danai Gkizi, Iakovos S. Pantelides, and Sotirios E. Tjamos. 2020. "The Ethylene Biosynthesis Genes ACS2 and ACS6 Modulate Disease Severity of Verticillium dahliae" Plants 9, no. 7: 907. https://doi.org/10.3390/plants9070907