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Article

Mutagenic Effect of Three Ion Beams on Rice and Identification of Heritable Mutations by Whole Genome Sequencing

1
National Key Laboratory of Rice Biology, Institute of Crop Sciences, Zhejiang University, Hangzhou 310058, China
2
Institute of Nuclear-Agricultural Sciences, Zhejiang University, Hangzhou 310058, China
3
Zhejiang Provincial Key Laboratory of Crop Germplasm, Zhejiang University, Hangzhou 310058, China
4
Jiaxing Academy of Agricultural Science, Jiaxing, Zhejiang 314016, China
5
Biophysics Group, Biomedical Research Center, Institute of Modern Physics, Chinese Academy of Science, Lanzhou 730000, China
6
Department of Basic Medical Sciences for Radiation Damages, National Institute of Radiological Sciences, National Institutes for Quantum and Radiological Science and Technology, Chiba 263-8555, Japan
*
Author to whom correspondence should be addressed.
Plants 2020, 9(5), 551; https://doi.org/10.3390/plants9050551
Received: 24 March 2020 / Revised: 22 April 2020 / Accepted: 23 April 2020 / Published: 26 April 2020
(This article belongs to the Special Issue Plant Mutation Breeding)
High-energy ion beams are known to be an effective and unique type of physical mutagen in plants. However, no study on the mutagenic effect of argon (Ar) ion beam radiation on rice has been reported. Genome-wide studies on induced mutations are important to comprehend their characteristics for establishing knowledge-based protocols for mutation induction and breeding, which are still very limited in rice. The present study aimed to investigate the mutagenic effect of three ion beams, i.e., Ar, carbon (C) and neon (Ne) on rice and identify and characterize heritable induced mutations by the whole genome sequencing of six M4 plants. Dose-dependent damage effects were observed on M1 plants, which were developed from ion beam irradiated dry seeds of two indica (LH15, T23) and two japonica (DS551, DS48) rice lines. High frequencies of chlorophyll-deficient seedlings and male-sterile plants were observed in all M2 populations (up to ~30% on M1 plant basis); plants from the seeds of different panicles of a common M1 plant appeared to have different mutations; the whole genome-sequencing demonstrated that there were 236–453 mutations in each of the six M4 plants, including single base substitutions (SBSs) and small insertion/deletions (InDels), with the number of SBSs ~ 4–8 times greater than that of InDels; SBS and InDel mutations were distributed across different genomic regions of all 12 chromosomes, however, only a small number of mutations (0–6) were present in exonic regions that might have an impact on gene function. In summary, the present study demonstrates that Ar, C and Ne ion beam radiation are all effective for mutation induction in rice and has revealed at the genome level the characteristics of the mutations induced by the three ion beams. The findings are of importance to the efficient use of ion beam radiation for the generation and utilization of mutants in rice. View Full-Text
Keywords: rice (Oryza sativa L.); mutagenesis; mutation breeding; ion beams; whole genome sequencing; mutation spectrum rice (Oryza sativa L.); mutagenesis; mutation breeding; ion beams; whole genome sequencing; mutation spectrum
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MDPI and ACS Style

Zheng, Y.; Li, S.; Huang, J.; Fu, H.; Zhou, L.; Furusawa, Y.; Shu, Q. Mutagenic Effect of Three Ion Beams on Rice and Identification of Heritable Mutations by Whole Genome Sequencing. Plants 2020, 9, 551. https://doi.org/10.3390/plants9050551

AMA Style

Zheng Y, Li S, Huang J, Fu H, Zhou L, Furusawa Y, Shu Q. Mutagenic Effect of Three Ion Beams on Rice and Identification of Heritable Mutations by Whole Genome Sequencing. Plants. 2020; 9(5):551. https://doi.org/10.3390/plants9050551

Chicago/Turabian Style

Zheng, Yunchao, Shan Li, Jianzhong Huang, Haowei Fu, Libin Zhou, Yoshiya Furusawa, and Qingyao Shu. 2020. "Mutagenic Effect of Three Ion Beams on Rice and Identification of Heritable Mutations by Whole Genome Sequencing" Plants 9, no. 5: 551. https://doi.org/10.3390/plants9050551

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