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Open AccessArticle

Leaf-Wounding Long-Distance Signaling Targets AtCuAOβ Leading to Root Phenotypic Plasticity

1
Department of Science, University “Roma Tre”, 00146 Rome, Italy
2
Department of Life, Health and Environmental Sciences, University of L’Aquila, 67100 L’Aquila, Italy
*
Author to whom correspondence should be addressed.
Plants 2020, 9(2), 249; https://doi.org/10.3390/plants9020249
Received: 23 December 2019 / Revised: 18 January 2020 / Accepted: 12 February 2020 / Published: 15 February 2020
(This article belongs to the Special Issue 2019 Feature Papers by Plants’ Editorial Board Members)
The Arabidopsis gene AtCuAOβ (At4g14940) encodes an apoplastic copper amine oxidase (CuAO) highly expressed in guard cells of leaves and flowers and in root vascular tissues, especially in protoxylem and metaxylem precursors, where its expression is strongly induced by the wound signal methyl jasmonate (MeJA). The hydrogen peroxide (H2O2) derived by the AtCuAOβ-driven oxidation of the substrate putrescine (Put), mediates the MeJA–induced early root protoxylem differentiation. Considering that early root protoxylem maturation was also induced by both exogenous Put and leaf wounding through a signaling pathway involving H2O2, in the present study we investigated the role of AtCuAOβ in the leaf wounding-induced early protoxylem differentiation in combination with Put treatment. Quantitative and tissue specific analysis of AtCuAOβ gene expression by RT-qPCR and promoter::green fluorescent protein-β-glucuronidase fusion analysis revealed that wounding of the cotiledonary leaf induced AtCuAOβ gene expression which was particularly evident in root vascular tissues. AtCuAOβ loss-of-function mutants were unresponsive to the injury, not showing altered phenotype upon wounding in comparison to wild type seedlings. Exogenous Put and wounding did not show synergy in inducing early root protoxylem maturation, suggesting their involvement in a shared signaling pathway. View Full-Text
Keywords: Copper amine oxidases; polyamines; hydrogen peroxide; wounding; root plasticity; protoxylem Copper amine oxidases; polyamines; hydrogen peroxide; wounding; root plasticity; protoxylem
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Fraudentali, I.; Rodrigues-Pousada, R.A.; Tavladoraki, P.; Angelini, R.; Cona, A. Leaf-Wounding Long-Distance Signaling Targets AtCuAOβ Leading to Root Phenotypic Plasticity. Plants 2020, 9, 249.

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