The Role of Symbiotic Microorganisms, Nutrient Uptake and Rhizosphere Bacterial Community in Response of Pea (Pisum sativum L.) Genotypes to Elevated Al Concentrations in Soil
Abstract
:1. Introduction
2. Results
2.1. Plant Biomass
2.2. Symbiotic Structures
2.3. Rhizosphere pH and Al Concentrations
2.4. Nitrogen Uptake
2.5. Phosphorus Uptake
2.6. Concentration of Other Nutrients in the Rhizosphere
2.7. Uptake of Other Nutrients by Plants
2.8. Rhizosphere Bacterial Communities
3. Discussion
3.1. Plant Biomass
3.2. Symbiotic Structures
3.3. Rhizosphere pH and Al Concentrations
3.4. Nitrogen Uptake
3.5. Phosphorus Uptake
3.6. Uptake of Other Nutrients by Plants
3.7. Rhizosphere Bacterial Communities
4. Materials and Methods
4.1. Plants and Microorganisms
4.2. Plant Growth Conditions
4.3. Symbiotic Parameters
4.4. DNA Extraction from Soil
4.5. Sequencing Data Processing
4.6. Aluminium and Nutrient Contents
4.7. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Treatments | Shoots | Seeds | ||||||
---|---|---|---|---|---|---|---|---|
N Concentration (mg g−1 DW) | 15N Fraction (%) | N Content (mg plant−1) | 15N Content (mg plant−1) | N Concentration (mg g−1 DW) | 15N Fraction (%) | N Content (mg plant−1) | 15N Content (mg plant−1) | |
Pea genotype VIR1903 | ||||||||
-Al −M | 7.1 ± 0.2 a | 3.50 ± 0.01 c | 36 ± 1 a | 1.25 ± 0.03 b | 27.9 ± 2.0 a | 2.60 ± 0.16 a | 123 ± 11 a | 3.19 ± 0.27 a |
-Al +M | 7.6 ± 0.1 a | 4.44 ± 0.02 d | 45 ± 3 b | 1.98 ± 0.13 d | 28.1 ± 1.3 a | 2.52 ± 0.21 a | 143 ± 17 ab | 3.51 ± 0.20 a |
+Al −M | 7.9 ± 0.1 a | 3.25 ± 0.01 b | 34 ± 2 a | 1.11 ± 0.07 a | 33.0 ± 1.6 b | 2.72 ± 0.18 a | 128 ± 7 ab | 3.46 ± 0.20 a |
+Al +M | 10.0 ± 0.2 b | 2.79 ± 0.03 a | 59 ± 3 c | 1.64 ± 0.08 c | 36.8 ± 1.8 c | 2.10 ± 0.20 a | 168 ± 6 b | 3.53 ± 0.35 a |
Pea genotype VIR7307 | ||||||||
-Al −M | 6.4 ± 0.1 ab | 2.02 ± 0.01 a | 40 ± 3 a | 0.81 ± 0.06 a | 33.6 ± 1.4 b | 1.31 ± 0.09 a | 301 ± 33 b | 3.87 ± 0.19 a |
-Al +M | 5.6 ± 0.2 a | 2.00 ± 0.01 a | 42 ± 1 ab | 0.84 ± 0.03 a | 32.8 ± 0.9 ab | 1.48 ± 0.12 a | 314 ± 21 b | 4.56 ± 0.09 a |
+Al −M | 7.1 ± 0.2 b | 2.61 ± 0.01 b | 43 ± 4 ab | 1.12 ± 0.12 b | 30.5 ± 1.1 ab | 2.38 ± 0.23 b | 199 ± 13 a | 4.73 ± 0.46 a |
+Al +M | 7.1 ± 0.1 b | 2.57 ± 0.01 b | 48 ± 2 b | 1.24 ± 0.06 b | 29.8 ± 0.6 a | 2.34 ± 0.24 b | 201 ± 15 a | 4.62 ± 0.28 a |
Pea genotype VIR8353 | ||||||||
-Al −M | 13.4 ± 0.1 a | 5.60 ± 0.01 d | 43 ± 1 b | 2.41 ± 0.07 b | 35.2 ± 0.8 a | 3.08 ± 0.34 b | 171 ± 20 a | 5.13 ± 0.43 c |
-Al +M | 13.3 ± 0.1 a | 2.72 ± 0.01 a | 44 ± 2 b | 1.21 ± 0.05 a | 38.7 ± 0.9 a | 1.82 ± 0.09 a | 220 ± 11 b | 4.04 ± 0.37 b |
+Al −M | 13.1 ± 0.3 a | 3.36 ± 0.01 c | 32 ± 3 a | 1.06 ± 0.09 a | 35.5 ± 1.2 a | 2.28 ± 0.25 a | 137 ± 15 a | 3.07 ± 0.35 a |
+Al +M | 13.8 ± 0.2 a | 3.12 ± 0.01 b | 37 ± 2 ab | 1.16 ± 0.05 a | 38.7 ± 0.6 a | 2.39 ± 0.20 a | 175 ± 12 a | 4.17 ± 0.44 b |
Pea genotype VIR8473 | ||||||||
-Al −M | 12.4 ± 0.2 b | 4.17 ± 0.01 b | 36 ± 2 b | 1.49 ± 0.08 b | 29.4 ± 2.0 a | 5.10 ± 0.17 b | 55 ± 6 ab | 2.81 ± 0.29 b |
-Al +M | 10.9 ± 0.7 ab | 3.42 ± 0.03 a | 34 ± 4 b | 1.10 ± 0.15 a | 30.4 ± 0.8 a | 3.98 ± 0.60 a | 96 ± 13 b | 3.61 ± 0.27 bc |
+Al −M | 10.3 ± 0.2 a | 4.36 ± 0.01 c | 23 ± 1 a | 1.01 ± 0.04 a | 33.0 ± 0.9 a | 4.12 ± 0.34 a | 44 ± 3 a | 1.78 ± 0.14 a |
+Al +M | 9.8 ± 0.9 a | 4.46 ± 0.01 d | 26 ± 2 a | 1.14 ± 0.10 a | 32.5 ± 2.0 a | 5.54 ± 0.16 b | 68 ± 4 ab | 3.74 ± 0.25 c |
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Belimov, A.A.; Shaposhnikov, A.I.; Syrova, D.S.; Kichko, A.A.; Guro, P.V.; Yuzikhin, O.S.; Azarova, T.S.; Sazanova, A.L.; Sekste, E.A.; Litvinskiy, V.A.; et al. The Role of Symbiotic Microorganisms, Nutrient Uptake and Rhizosphere Bacterial Community in Response of Pea (Pisum sativum L.) Genotypes to Elevated Al Concentrations in Soil. Plants 2020, 9, 1801. https://doi.org/10.3390/plants9121801
Belimov AA, Shaposhnikov AI, Syrova DS, Kichko AA, Guro PV, Yuzikhin OS, Azarova TS, Sazanova AL, Sekste EA, Litvinskiy VA, et al. The Role of Symbiotic Microorganisms, Nutrient Uptake and Rhizosphere Bacterial Community in Response of Pea (Pisum sativum L.) Genotypes to Elevated Al Concentrations in Soil. Plants. 2020; 9(12):1801. https://doi.org/10.3390/plants9121801
Chicago/Turabian StyleBelimov, Andrey A., Alexander I. Shaposhnikov, Darya S. Syrova, Arina A. Kichko, Polina V. Guro, Oleg S. Yuzikhin, Tatiana S. Azarova, Anna L. Sazanova, Edgar A. Sekste, Vladimir A. Litvinskiy, and et al. 2020. "The Role of Symbiotic Microorganisms, Nutrient Uptake and Rhizosphere Bacterial Community in Response of Pea (Pisum sativum L.) Genotypes to Elevated Al Concentrations in Soil" Plants 9, no. 12: 1801. https://doi.org/10.3390/plants9121801