Changes in Beneficial C-glycosylflavones and Policosanol Content in Wheat and Barley Sprouts Subjected to Differential LED Light Conditions
Abstract
:1. Introduction
2. Results
2.1. Changes of C-glycosylflavone Content in Barley and Wheat Seedlings Exposed to Differential LED Light Irradiation
2.2. Effect of LED Light Irradiation on Major Long-Chain Fatty-Alcohol (Policosanol) Biosynthesis in Barley and Wheat Seedlings
2.3. Effect of Growth Periods on C-glycosylflavones and Policosanol Content in Young Barley and Wheat Seedlings
2.4. Expression Profiling of Potential Genes Involved in the Biosynthesis of C-glycosylflavones/Flavonoids and Policosanols in Barley Seedlings under Different Light Conditions and Growth Times
2.5. Influence of LED-Light Irradiation on Seedling Growth
3. Discussion
4. Materials and Methods
4.1. Plant Materials, Growth Condition, and LED Treatment
4.2. Extraction of C-glycosylated Flavones and Measurement
4.3. Extraction and Quantification of Policosanols from Barley and Wheat Seedlings
4.4. Identification of Saponarin and Policosanol Biosynthetic-Related Genes
4.5. Analysis of Expression Pattern of Biosynthetic Related Genes in Seedlings Subjected to LED Light Irradiation
4.6. Phenotyping of LED-Treated Cereal Sprouts
4.7. Statistical Analyses
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Metabolite Content vs. Gene Expression | ||||||||
---|---|---|---|---|---|---|---|---|
HvOGT1 | HvFNSII | HvCHS1 | HvFAR2 | HvFAR3 | HvFAR4 | HvFAR5 | HvFAR6 | |
Saponarin | 0.68 (p = 0.01) ** | −0.53 (p = 0.07) | 0.13 (p = 0.68) | -- | -- | -- | -- | -- |
Hexacosanol | -- | -- | -- | −0.59 (p = 0.04) * | 0.67 ( p= 0.01) ** | −0.62 (p = 0.03) * | −0.76 (p = 0.004) ** | −0.69 (p = 0.01) ** |
Growth periods (–9 days) vs. metabolite content | ||||||||
Fluorescent | White | Blue | Red | |||||
Saponarin | −0.93 (p = 0.00001) **** | −0.99 (p = 0.00001) **** | −0.99 (p = 0.00001) **** | −0.97 (p = 0.00001) **** | ||||
Isoorientin | −0.82 (p = 0.001) *** | −0.92 (p = 0.00002) **** | −0.23 (p = 0.47) | −0.66 (p = 0.01) ** | ||||
Isoschaftoside | −0.32 (p = 0.31) | −0.94 (p = 0.00001) **** | −0.47 (p = 0.12) | −0.90 (p = 0.00006) **** | ||||
Hexacosanol | 0.79 (p = 0.001) *** | 0.69 (p = 0.01) ** | 0.91 (p = 0.00004) **** | 0.81 (p = 0.001) *** | ||||
Octacosanol | −0.07 (p = 0.82) | −0.43 (p = 0.16) | −0.17 (p = 0.57) | −0.04 (p = 0.90) |
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Muthusamy, M.; Kim, J.H.; Kim, S.H.; Kim, J.Y.; Heo, J.W.; Lee, H.; Lee, K.-S.; Seo, W.D.; Park, S.; Kim, J.A.; et al. Changes in Beneficial C-glycosylflavones and Policosanol Content in Wheat and Barley Sprouts Subjected to Differential LED Light Conditions. Plants 2020, 9, 1502. https://doi.org/10.3390/plants9111502
Muthusamy M, Kim JH, Kim SH, Kim JY, Heo JW, Lee H, Lee K-S, Seo WD, Park S, Kim JA, et al. Changes in Beneficial C-glycosylflavones and Policosanol Content in Wheat and Barley Sprouts Subjected to Differential LED Light Conditions. Plants. 2020; 9(11):1502. https://doi.org/10.3390/plants9111502
Chicago/Turabian StyleMuthusamy, Muthusamy, Jong Hee Kim, Suk Hee Kim, Joo Yeol Kim, Jeong Wook Heo, HanGyeol Lee, Kwang-Sik Lee, Woo Duck Seo, Soyoung Park, Jin A Kim, and et al. 2020. "Changes in Beneficial C-glycosylflavones and Policosanol Content in Wheat and Barley Sprouts Subjected to Differential LED Light Conditions" Plants 9, no. 11: 1502. https://doi.org/10.3390/plants9111502