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Article

LED Lights Affecting Morphogenesis and Isosteroidal Alkaloid Contents in Fritillaria cirrhosa D. Don—An Important Chinese Medicinal Herb

by
Chia-Chen Chen
1,
Maw-Rong Lee
2,
Chi-Rei Wu
1,
Hsin-Ju Ke
2,
Hui-Min Xie
3,
Hsin-Sheng Tsay
4,
Dinesh Chandra Agrawal
4,* and
Hung-Chi Chang
5,*
1
Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, China Medical University, Taichung 40402, Taiwan
2
Department of Chemistry, National Chung-Hsing University, Taichung 40227, Taiwan
3
Nin Jiom Pharmaceutical Co. Ltd., Taipei 108024, Taiwan
4
Department of Applied Chemistry, Chaoyang University of Technology, Taichung 41349, Taiwan
5
Department of Golden-Ager Industry Management, Chaoyang University of Technology, Taichung 41349, Taiwan
*
Authors to whom correspondence should be addressed.
Plants 2020, 9(10), 1351; https://doi.org/10.3390/plants9101351
Submission received: 12 August 2020 / Revised: 6 October 2020 / Accepted: 7 October 2020 / Published: 13 October 2020
(This article belongs to the Special Issue The Effects of LED Light Spectra and Intensities on Plant Growth)
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Abstract

Investigations were carried out to study the effects of light-emitting diode (LED) lights on growth and development of isosteroidal alkaloids in embryogenic calli of Fritillaria cirrhosa D. Don, an important traditional Chinese medicine herb. Calli were cultured in glass bottles, each containing 100 mL of Murashige and Skoog’s basal medium supplemented with 2% sucrose and 0.4% gellan gum powder, a gelling agent. These bottles were incubated in a specially designed plant growth chamber equipped with eight different LED lights consisting of single or combinations of four different light spectra emitting blue (450 nm), green (525 nm), red (660 nm), and far-red (730 nm) light. After three months of incubation, morphological changes in embryogenic calli were recorded, and LC-MS/MS analysis of cultures was carried out for peimisine, sipeimine, peiminine, and peimine. The highest number of somatic embryos and the maximum fresh weight was recorded in calli incubated under red (9R), infrared (9IR), and a combination of red+blue+infrared (3R3B3IR), respectively, in decreasing order. The highest contents of peimisine, peiminine, and peimine were recorded under red (9R) and infrared (9IR) lights, respectively. Eight LED lights had significant effects on the morphogenesis of embryogenic calli of F. cirrhosa D. Don and contents of isosteroidal alkaloids.
Keywords: Fritillaria cirrhosa D. Don; alkaloid content; callus; in vitro culture; LED lights; light intensity Fritillaria cirrhosa D. Don; alkaloid content; callus; in vitro culture; LED lights; light intensity

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MDPI and ACS Style

Chen, C.-C.; Lee, M.-R.; Wu, C.-R.; Ke, H.-J.; Xie, H.-M.; Tsay, H.-S.; Agrawal, D.C.; Chang, H.-C. LED Lights Affecting Morphogenesis and Isosteroidal Alkaloid Contents in Fritillaria cirrhosa D. Don—An Important Chinese Medicinal Herb. Plants 2020, 9, 1351. https://doi.org/10.3390/plants9101351

AMA Style

Chen C-C, Lee M-R, Wu C-R, Ke H-J, Xie H-M, Tsay H-S, Agrawal DC, Chang H-C. LED Lights Affecting Morphogenesis and Isosteroidal Alkaloid Contents in Fritillaria cirrhosa D. Don—An Important Chinese Medicinal Herb. Plants. 2020; 9(10):1351. https://doi.org/10.3390/plants9101351

Chicago/Turabian Style

Chen, Chia-Chen, Maw-Rong Lee, Chi-Rei Wu, Hsin-Ju Ke, Hui-Min Xie, Hsin-Sheng Tsay, Dinesh Chandra Agrawal, and Hung-Chi Chang. 2020. "LED Lights Affecting Morphogenesis and Isosteroidal Alkaloid Contents in Fritillaria cirrhosa D. Don—An Important Chinese Medicinal Herb" Plants 9, no. 10: 1351. https://doi.org/10.3390/plants9101351

APA Style

Chen, C.-C., Lee, M.-R., Wu, C.-R., Ke, H.-J., Xie, H.-M., Tsay, H.-S., Agrawal, D. C., & Chang, H.-C. (2020). LED Lights Affecting Morphogenesis and Isosteroidal Alkaloid Contents in Fritillaria cirrhosa D. Don—An Important Chinese Medicinal Herb. Plants, 9(10), 1351. https://doi.org/10.3390/plants9101351

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