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Open AccessArticle

Stomatal and Photosynthetic Traits Are Associated with Investigating Sodium Chloride Tolerance of Brassica napus L. Cultivars

1
College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
2
Faculty of Agriculture, Fayoum University, Fayoum 63514, Egypt
*
Author to whom correspondence should be addressed.
Plants 2020, 9(1), 62; https://doi.org/10.3390/plants9010062
Received: 27 November 2019 / Revised: 19 December 2019 / Accepted: 28 December 2019 / Published: 2 January 2020
(This article belongs to the Special Issue Physiological and Molecular Plant Responses to Salt Stress)
The negative effects of salt stress vary among different rapeseed cultivars. In this study, we investigated the sodium chloride tolerance among 10 rapeseed cultivars based on membership function values (MFV) and Euclidean cluster analyses by exposing seedlings to 0, 100, or 200 mM NaCl. The NaCl toxicity significantly reduced growth, biomass, endogenous K+ levels, relative water content and increased electrolyte leakage, soluble sugar levels, proline levels, and antioxidant enzyme activities. SPAD values were highly variable among rapeseed cultivars. We identified three divergent (tolerant, moderately tolerant, and sensitive) groups. We found that Hua6919 and Yunyoushuang2 were the most salt-tolerant cultivars and that Zhongshuang11 and Yangyou9 were the most salt-sensitive cultivars. The rapeseed cultivars were further subjected to photosynthetic gas exchange and anatomical trait analyses. Among the photosynthetic gas exchange and anatomical traits, the stomatal aperture was the most highly correlated with salinity tolerance in rapeseed cultivars and thus, is important for future studies that aim to improve salinity tolerance in rapeseed. Thus, we identified and characterized two salt-tolerant cultivars that will be useful for breeding programs that aim to develop salt-tolerant rapeseed. View Full-Text
Keywords: Brassica napus; salinity; cluster analysis; stomata; growth; antioxidant enzymes Brassica napus; salinity; cluster analysis; stomata; growth; antioxidant enzymes
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Mohamed, I.A.A.; Shalby, N.; Bai, C.; Qin, M.; Agami, R.A.; Jie, K.; Wang, B.; Zhou, G. Stomatal and Photosynthetic Traits Are Associated with Investigating Sodium Chloride Tolerance of Brassica napus L. Cultivars. Plants 2020, 9, 62.

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    Description: Figure S1: Effect of different salt stress levels on chlorophyll content (SPDA). Figure S2: Effect of different salt stress levels on (A) K+ concentration, (B) total soluble sugar, and (C) proline concentration of B. napus L. cultivars. Figure S3: Effect of different salt stress levels on (A) relative water content (RWC%) and (B) electrolyte leakage (EL%) on B. napus L. cultivars. Figure S4. Effect of salt stress on antioxidants enzymes; (A) superoxide dismutase, (B) catalase, (C) ascorbate peroxidase, and (D) peroxidase of B. napus L. cultivars. Figure S5. Effect of salt stress on (A) net photosynthetic rate, (B) transpiration rate, and (C) stomatal conductance in B. napus L. cultivars. Figure S6. Effect of salt stress on (A) stomata aperture area and (B) stomata density in B. napus L. cultivars. Table S1. Effect of salt stress on plant height, number of leaves, and total leaves area of B. napus L. cultivars. Table S2. Effect of salt stress on the shoot and root fresh weights and shoot and root dry weights of B. napus L. cultivars. Table S3. Membership function value (MFV) of rapeseed cultivars.
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