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Open AccessArticle

Reactive Oxygen Species Alleviate Cell Death Induced by Thaxtomin A in Arabidopsis thaliana Cell Cultures

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Centre SÈVE, Département de Biologie, Université de Sherbrooke, Sherbrooke, QC J1K 2R1, Canada
2
Groupe de Recherche en Biologie Végétale, Département de Chimie, Biochimie et Physique, Université du Québec à Trois-Rivières, Trois-Rivières, QC G9A 5H7, Canada
3
Institut de Pharmacologie de Sherbrooke, Département de Pharmacologie et Physiologie, Université de Sherbrooke, Sherbrooke, QC J1H 5N4, Canada
*
Author to whom correspondence should be addressed.
Plants 2019, 8(9), 332; https://doi.org/10.3390/plants8090332
Received: 28 June 2019 / Revised: 26 August 2019 / Accepted: 3 September 2019 / Published: 6 September 2019
(This article belongs to the Special Issue ROS Responses in Plants)
Thaxtomin A (TA) is a cellulose biosynthesis inhibitor synthesized by the soil actinobacterium Streptomyces scabies, which is the main causal agent of potato common scab. TA is essential for the induction of scab lesions on potato tubers. When added to Arabidopsis thaliana cell cultures, TA induces an atypical programmed cell death (PCD). Although production of reactive oxygen species (ROS) often correlates with the induction of PCD, we observed a decrease in ROS levels following TA treatment. We show that this decrease in ROS accumulation in TA-treated cells is not due to the activation of antioxidant enzymes. Moreover, Arabidopsis cell cultures treated with hydrogen peroxide (H2O2) prior to TA treatment had significantly fewer dead cells than cultures treated with TA alone. This suggests that H2O2 induces biochemical or molecular changes in cell cultures that alleviate the activation of PCD by TA. Investigation of the cell wall mechanics using atomic force microscopy showed that H2O2 treatment can prevent the decrease in cell wall rigidity observed after TA exposure. While we cannot exclude the possibility that H2O2 may promote cell survival by altering the cellular redox environment or signaling pathways, our results suggest that H2O2 may inhibit cell death, at least partially, by reinforcing the cell wall to prevent or compensate for damages induced by TA. View Full-Text
Keywords: cell wall rigidity; H2O2; programmed cell death; thaxtomin A cell wall rigidity; H2O2; programmed cell death; thaxtomin A
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MDPI and ACS Style

Awwad, F.; Bertrand, G.; Grandbois, M.; Beaudoin, N. Reactive Oxygen Species Alleviate Cell Death Induced by Thaxtomin A in Arabidopsis thaliana Cell Cultures. Plants 2019, 8, 332.

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