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Isolation of Native Arbuscular Mycorrhizal Fungi within Young Thalli of the Liverwort Marchantia paleacea

1
Laboratory of Crop Nutrition, Department of Sustainable Agriculture, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, Japan
2
Hokkaido Agricultural Research Center, National Agriculture and Food Research Organization (NARO), 1 Hitsujigaoka, Toyohira, Sapporo, Hokkaido 062-8555, Japan
3
Central Region Agricultural Research Center, NARO, Kannondai 2-1-18, Tsukuba 305-8666, Japan
4
NARO Headquarters, Kannondai 3-1-1, Tsukuba 305-8517, Japan
5
Division of Symbiotic Systems, National Institute for Basic Biology, Nishigonaka 38, Myodaiji, Okazaki 444-8585, Aichi, Japan
6
Graduate School of Life Sciences, Tohoku University, Katahira 2-1-1, Aoba-Ku, Sendai 980-8577, Japan
*
Author to whom correspondence should be addressed.
Plants 2019, 8(6), 142; https://doi.org/10.3390/plants8060142
Received: 22 April 2019 / Revised: 23 May 2019 / Accepted: 28 May 2019 / Published: 30 May 2019
(This article belongs to the Special Issue Contribution of Mycorrhizal Symbiosis to Plant Growth)
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Abstract

Arbuscular mycorrhizal fungi (AMF) are a group of soil microorganisms that establish symbioses with most land plant species. “Root trap culture” generally has been used for isolating a single regenerated spore in order to establish a monospecific, native AMF line. Roots may be co-colonized with multiple AMF species; however, only a small portion of AMF within roots sporulate, and do so only under certain conditions. In this study, we tested whether young thalli (<2 mm) of the liverwort Marchantia paleacea harbour monospecific AMF, and can be used as a vegetative inoculant line. When M. paleacea gemmae were co-cultivated with roots obtained from the field, the young thalli were infected by AMF via rhizoids and formed arbuscules after 18 days post-sowing. Ribosomal DNA sequencing of the AMF-colonized thalli (mycothalli) revealed that they harboured phylogenetically diverse AMF; however, new gemmae sown around transplanted mycothalli showed evidence of colonization from phylogenetically uniform Rhizophagus species. Of note, mycothalli can also be used as an inoculum. These results suggest that the young thalli of M. paleacea can potentially isolate monospecific AMF from field soil in a spore-independent manner. View Full-Text
Keywords: liverwort; Marchantia paleacea; mycothalli; native arbuscular mycorrhizal fungi; Sanger sequencing liverwort; Marchantia paleacea; mycothalli; native arbuscular mycorrhizal fungi; Sanger sequencing
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Kobae, Y.; Ohtomo, R.; Morimoto, S.; Sato, D.; Nakagawa, T.; Oka, N.; Sato, S. Isolation of Native Arbuscular Mycorrhizal Fungi within Young Thalli of the Liverwort Marchantia paleacea. Plants 2019, 8, 142.

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