Isolation of Native Arbuscular Mycorrhizal Fungi within Young Thalli of the Liverwort Marchantia paleacea
Abstract
:1. Introduction
2. Results and Discussion
2.1. Growth Condition of M. paleacea Young Thalli
2.2. Young Thalli (<2 mm) Colonized by Arbuscular Mycorrhizal Fungi
2.3. First Trapping of Arbuscular Mycorrhizal Fungi and Sequencing
2.4. “Meta”-Sequences of Arbuscular Mycorrhizal Fungi rDNA in Young Thalli
2.5. Second Trapping Isolated Phylogenetically Uniform Arbuscular Mycorrhizal Fungi Species
2.6. Mycothalli Can Be Used as an Arbuscular Mycorrhizal Fungi Inoculum in Pot Experiments
3. Materials and Methods
3.1. M. paleacea Thalli Growth Conditions
3.2. Inoculation Test
3.3. Arbuscular Mycorrhizal Fungi Cell Wall Staining
3.4. Trap Culture
3.5. PCR and Sequencing
3.6. Sequence Analysis and Classification
3.7. Mycothalli Inoculation Test
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Kobae, Y.; Ohtomo, R.; Morimoto, S.; Sato, D.; Nakagawa, T.; Oka, N.; Sato, S. Isolation of Native Arbuscular Mycorrhizal Fungi within Young Thalli of the Liverwort Marchantia paleacea. Plants 2019, 8, 142. https://doi.org/10.3390/plants8060142
Kobae Y, Ohtomo R, Morimoto S, Sato D, Nakagawa T, Oka N, Sato S. Isolation of Native Arbuscular Mycorrhizal Fungi within Young Thalli of the Liverwort Marchantia paleacea. Plants. 2019; 8(6):142. https://doi.org/10.3390/plants8060142
Chicago/Turabian StyleKobae, Yoshihiro, Ryo Ohtomo, Sho Morimoto, Daiki Sato, Tomomi Nakagawa, Norikuni Oka, and Shusei Sato. 2019. "Isolation of Native Arbuscular Mycorrhizal Fungi within Young Thalli of the Liverwort Marchantia paleacea" Plants 8, no. 6: 142. https://doi.org/10.3390/plants8060142
APA StyleKobae, Y., Ohtomo, R., Morimoto, S., Sato, D., Nakagawa, T., Oka, N., & Sato, S. (2019). Isolation of Native Arbuscular Mycorrhizal Fungi within Young Thalli of the Liverwort Marchantia paleacea. Plants, 8(6), 142. https://doi.org/10.3390/plants8060142