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Open AccessArticle

Non-cultivated Cotton Species (Gossypium spp.) Act as a Reservoir for Cotton Leaf Curl Begomoviruses and Associated Satellites

1
National Institute for Biotechnology and Genetic Engineering, 38000 Faisalabad, Pakistan
2
Virology Lab, Centre for Agricultural Biochemistry and Biotechnology, University of Agriculture, 38000 Faisalabad, Pakistan
3
Crop Genetics Research Unit, USDA-ARS, 141 Experiment Station Rd, Stoneville, MS 38776, USA
4
Genomics and Bioinformatics Research Unit, USDA-ARS, 141 Experiment Station Rd, Stoneville, MS 38776, USA
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Plants 2019, 8(5), 127; https://doi.org/10.3390/plants8050127
Received: 28 March 2019 / Revised: 2 May 2019 / Accepted: 10 May 2019 / Published: 14 May 2019
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Abstract

A collection of cultivated and non-cultivated species of cotton (Gossypium spp.) has been maintained for the last four decades in Multan, Pakistan. This geographical location has been observed as a hotspot for the evolution of begomoviruses and satellites associated with cotton leaf curl disease (CLCuD). Recent studies showed that begomoviruses responsible for the CLCuD epidemic in the 1990s, and that almost disappeared from the CLCuD complex in 2000s, have been observed again in CLCuD-infected cotton fields. To identify host species that acted as probable reservoirs for these viruses, we characterized begomoviruses and satellites in non-cultivated cotton species G. raimondii, G. thurberi and G. mustelinum and identified several species of CLCuD associated begomoviruses and satellites. Further, phylogenetic analysis indicated that the identified begomoviruses and beta/alphasatellites are closely related to the ones associated with the most recent CLCuD complex. qPCR indicated that the comparative level of virus significantly decreased in the presence of alphasatellites. Our results indicated that non-cultivated cotton species have been continuously challenged by diverse begomoviruses and associated satellites and act as reservoirs for CLCuD associated begomoviruses. These results provide novel insights into understanding the spread of begomoviruses and associated satellites in New World cotton species introduced into the Old World. View Full-Text
Keywords: Alphasatellite; Begomovirus; Betasatellite; Cotton leaf curl disease; Gossypium spp. Alphasatellite; Begomovirus; Betasatellite; Cotton leaf curl disease; Gossypium spp.
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Shakir, S.; Zaidi, S. .-E.-A.; Atiq-ur-Rehman; Farooq, M.; Amin, I.; Scheffler, J.; Scheffler, B.; Shah Nawaz-ul-Rehman, M.; Mansoor, S. Non-cultivated Cotton Species (Gossypium spp.) Act as a Reservoir for Cotton Leaf Curl Begomoviruses and Associated Satellites. Plants 2019, 8, 127.

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