Contribution of Root Hair Development to Sulfate Uptake in Arabidopsis
Abstract
:1. Introduction
2. Results
2.1. SULTR1;1 and SULTR1;2 Were Differentially Expressed in the Root Epidermal Cells under −S
2.2. Primary Root Length and Numbers of Root Hairs Compensated for Each Other in wer and cpc
2.3. Regulatory Gene Expression in Root Hair Development Was Consistent with Root Hair Numbers under +S but Not –S Conditions
2.4. Sulfate Uptake Was Constant among WT, wer, and cpc
3. Discussion
4. Materials and Methods
4.1. Plant Materials and Growth Conditions
4.2. Observation of GFP Fluorescence
4.3. Observation of Root Development
4.4. Quantitative Real-Time RT-PCR Analysis
4.5. Sulfate Uptake Assay
4.6. Measurement of Sulfate, Cysteine and Glutathione, and Total Sulfur Levels
4.7. Statistical Analysis
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Kimura, Y.; Ushiwatari, T.; Suyama, A.; Tominaga-Wada, R.; Wada, T.; Maruyama-Nakashita, A. Contribution of Root Hair Development to Sulfate Uptake in Arabidopsis. Plants 2019, 8, 106. https://doi.org/10.3390/plants8040106
Kimura Y, Ushiwatari T, Suyama A, Tominaga-Wada R, Wada T, Maruyama-Nakashita A. Contribution of Root Hair Development to Sulfate Uptake in Arabidopsis. Plants. 2019; 8(4):106. https://doi.org/10.3390/plants8040106
Chicago/Turabian StyleKimura, Yuki, Tsukasa Ushiwatari, Akiko Suyama, Rumi Tominaga-Wada, Takuji Wada, and Akiko Maruyama-Nakashita. 2019. "Contribution of Root Hair Development to Sulfate Uptake in Arabidopsis" Plants 8, no. 4: 106. https://doi.org/10.3390/plants8040106