Next Article in Journal
Traditional Medicine Plant, Onopordum acanthium L. (Asteraceae): Chemical Composition and Pharmacological Research
Previous Article in Journal
Using Morphogenic Genes to Improve Recovery and Regeneration of Transgenic Plants
Article Menu
Issue 2 (February) cover image

Export Article

Open AccessArticle
Plants 2019, 8(2), 39;

PbCOP1.1 Contributes to the Negative Regulation of Anthocyanin Biosynthesis in Pear

College of Horticulture, Northwest A&F University, Taicheng Road NO.3, Yangling 712100, China
Authors to whom correspondence should be addressed.
Received: 26 December 2018 / Revised: 30 January 2019 / Accepted: 30 January 2019 / Published: 12 February 2019
Full-Text   |   PDF [2346 KB, uploaded 12 February 2019]   |  


The synthesis of anthocyanin in pear (Pyrus bretschneideri) fruit is regulated by light. However, little is known about the molecular mechanisms of pear fruit coloring mediated by upstream light-signaling regulators. Here, the photoresponse factors CONSTITUTIVE PHOTOMORPHOGENIC (COP) 1.1 and 1.2 were cloned from ‘Red Zaosu’ peel to study their functions in pear fruit coloring. The overexpression vectors pBI121-PbCOP1.1 and pBI121-PbCOP1.2 were constructed to analyze their effects on anthocyanin synthesis in pear fruit. A protein sequence alignment and phylogenetic tree analysis revealed that PbCOP1 proteins are highly homologous with those of other species. An analysis of tissue differential expression showed that the greatest expression levels of PbCOP1s occurred in the leaves. Their expression levels increased in the leaves during development, when the leaves changed from red to green. The overexpression of PbCOP1s in the peel resulted in reduced anthocyanin synthesis at the injection sites. A quantitative PCR analysis of the injection sites showed that PbCOP1.1 significantly inhibited the expression of the anthocyanin synthesis-related genes CHI, DFR, UFGT2, bHLH3, HY5 and GST. Based on the above results, we hypothesize that PbCOP1.1 is an anthocyanin synthetic inhibitory factor of pear coloration. View Full-Text
Keywords: pear; PbCOP1; anthocyanin; transient expression; color fading pear; PbCOP1; anthocyanin; transient expression; color fading

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material


Share & Cite This Article

MDPI and ACS Style

Wu, M.; Si, M.; Li, X.; Song, L.; Liu, J.; Zhai, R.; Cong, L.; Yue, R.; Yang, C.; Ma, F.; Xu, L.; Wang, Z. PbCOP1.1 Contributes to the Negative Regulation of Anthocyanin Biosynthesis in Pear. Plants 2019, 8, 39.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Plants EISSN 2223-7747 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top