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Open AccessArticle

Exogenous Nitric Oxide Mitigates Nickel-Induced Oxidative Damage in Eggplant by Upregulating Antioxidants, Osmolyte Metabolism, and Glyoxalase Systems

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Botany and Microbiology Department, Faculty of Science, Cairo University, 12613, Giza, Egypt
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Department of Biology, College of science, Jouf University, Sakaka 2014, Saudi Arabia
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Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah 21577, Saudi Arabia
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Princess Dr Najla Bint Saud Al- Saud Center for Excellence Research in Biotechnology, King Abdulaziz University, P.O. Box 80200, Jeddah, Saudi Arabia
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Department of Biology, Faculty of Science, University of Tabuk, Tabuk-71491, Saudi Arabia
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Botany Department, Faculty of Science, Tanta University, 31527 Tanta, Egypt
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Sainsbury Laboratory, University of Cambridge, Cambridge CB2 1LR, UK
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Botany Department, Faculty of Science, Suez Canal University, Ismailia 41522, Egypt
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Author to whom correspondence should be addressed.
Plants 2019, 8(12), 562; https://doi.org/10.3390/plants8120562
Received: 7 November 2019 / Revised: 25 November 2019 / Accepted: 28 November 2019 / Published: 1 December 2019
(This article belongs to the Special Issue ROS Responses in Plants)
Nitric oxide (NO) at optimal levels is considered beneficial to plant functioning. The present study was carried out to investigate the role of exogenously applied NO (100 and 150 µM sodium nitropurusside, SNP) in amelioration of nickel (Ni)-mediated oxidative effects in eggplant. Ni stress declined growth and biomass production, relative water content (RWC), and chlorophyll pigment synthesis, thereby affecting the photosynthetic efficiency. Exogenously applied SNP proved beneficial in mitigating the Ni-mediated growth restrictions. NO-treated seedlings exhibited improved photosynthesis, stomatal conductance, and chlorophyll content with the effect of being apparent at lower concentration (100 µM SNP). SNP upregulated the antioxidant system mitigating the oxidative damage on membranes due to Ni stress. The activity of superoxide dismutase, catalase, glutathione S-transferase, ascorbate peroxidase, and glutathione reductase was upregulated due to SNP which also increased the ascorbate and reduced glutathione content. SNP-supplied seedlings also showed higher proline and glycine betaine accumulation, thereby improving RWC and antioxidant system. Glyoxalase I activity was induced due to SNP application declining the accumulation of methylglyoxal. NO-mediated mitigation of Ni toxicity was confirmed using NO scavenger (PTIO, 2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide), which reversed the influence of SNP almost entirely on the parameters studied. Uptake of nitrogen (N), potassium (K), and calcium (Ca) was increased due to SNP application and Ni was reduced significantly. Therefore, this study revealed the efficiency of exogenous SNP in enhancing Ni stress tolerance through upregulating antioxidant and glyoxalase systems.
Keywords: Antioxidants; Glyoxalase; Lipid peroxidation; Lipoxygenase; Nickel; Nitric oxide Antioxidants; Glyoxalase; Lipid peroxidation; Lipoxygenase; Nickel; Nitric oxide
MDPI and ACS Style

Soliman, M.; Alhaithloul, H.A.; Hakeem, K.R.; Alharbi, B.M.; El-Esawi, M.; Elkelish, A. Exogenous Nitric Oxide Mitigates Nickel-Induced Oxidative Damage in Eggplant by Upregulating Antioxidants, Osmolyte Metabolism, and Glyoxalase Systems. Plants 2019, 8, 562.

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