Next Article in Journal
Organogenesis at the Shoot Apical Meristem
Next Article in Special Issue
The Costs of Green Leaf Volatile-Induced Defense Priming: Temporal Diversity in Growth Responses to Mechanical Wounding and Insect Herbivory
Previous Article in Journal
Drawing a Line: Grasses and Boundaries
Previous Article in Special Issue
Should I Eat or Should I Go? Acridid Grasshoppers and Their Novel Host Plants: Potential for Biotic Resistance
Open AccessArticle

Isolation and Characterization of Plant Growth-Promoting Endophytic Fungi from the Roots of Dendrobium moniliforme

Central Department of Botany, Tribhuvan University, Kathmandu 2642, Nepal
Institut de Systématique, Évolution, Biodiversité (UMR 7205—CNRS, MNHN, UPMC, EPHE), Muséum National d’Histoire naturelle, Sorbonne Universités, 57 rue Cuvier, 75005 Paris, France
Department of Plant Taxonomy and Nature Conservation, University of Gdansk, Wita Stwosza 59, 80-308 Gdansk, Poland
Author to whom correspondence should be addressed.
Received: 25 August 2018 / Revised: 6 November 2018 / Accepted: 10 November 2018 / Published: 28 December 2018
(This article belongs to the Special Issue Plants Interacting with other Organisms: Insects)
The present study aims to identify the diverse endophytic fungi residing in the roots of Dendrobium moniliforme and their role in plant growth and development. Nine endophytic fungi were isolated from the root sections and characterized by molecular technique. Quantification of the indole acetic acid (IAA) compound by these endophytes was done. Further, Chemical profiling of R11 and R13 fungi was done by Gas Chromatography-Mass Spectroscopy (GC-MS). Asymbiotic seed derived protocorms of Rhynchostylis retusa was used for the plant growth assay to investigate the growth promoting activities of the fungal elicitor prepared from the isolated fungi from D. moniliforme. Among the isolated fungi, the relative dominant fungus was Fusarium sp. The R13 and R6 fungi were identified only at the genus level which concludes the fungi are of new species or strain. The indole acetic acid production was relatively higher in R10. Bioactive compound diversity was observed in the organic extract of R11 and R6. The presence of phenolic compound and essential oil suggest their contribution for the antimicrobial and antioxidant properties to their host plant, D. moniliforme. The plant growth assay result concluded, the fungal elicitor prepared from R10, Colletotrichum alatae was the best among all other for the plant growth activities. View Full-Text
Keywords: orchid; Dendrobium moniliforme; endophytes; elicitor; indole acetic acid orchid; Dendrobium moniliforme; endophytes; elicitor; indole acetic acid
Show Figures

Figure 1

MDPI and ACS Style

Shah, S.; Shrestha, R.; Maharjan, S.; Selosse, M.-A.; Pant, B. Isolation and Characterization of Plant Growth-Promoting Endophytic Fungi from the Roots of Dendrobium moniliforme. Plants 2019, 8, 5.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

Search more from Scilit
Back to TopTop