Chemical Compositions of Scutellaria Essential Oils Cultivated in Eastern Oregon: S. angustifolia, S. baicalensis, S. barbata, and S. lateriflora
Abstract
1. Introduction
2. Results
3. Discussion
4. Materials and Methods
4.1. Plant Material
4.2. Hydrodistillation
4.3. Gas Chromatographic Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
- World Flora Online. An Online Flora of All Known Plants. Available online: https://www.worldfloraonline.org/ (accessed on 24 November 2025).
- Li, C.; Lin, G.; Zuo, Z. Pharmacological Effects and Pharmacokinetics Properties of Radix scutellariae and Its Bioactive Flavones. Biopharm. Drug Dispos. 2011, 32, 427–445. [Google Scholar] [CrossRef] [PubMed]
- Shah, M.; Mubin, S.; ul Hassan, S.S.; Tagde, P.; Ullah, O.; Rahman, M.H.; Al-Harrasi, A.; Ur Rehman, N.; Murad, W. Phytochemical Profiling and Bio-Potentiality of Genus Scutellaria: Biomedical Approach. Biomolecules 2022, 12, 936. [Google Scholar] [CrossRef] [PubMed]
- Zhao, T.; Tang, H.; Xie, L.; Zheng, Y.; Ma, Z.; Sun, Q.; Li, X. Scutellaria baicalensis Georgi. (Lamiaceae): A Review of Its Traditional Uses, Botany, Phytochemistry, Pharmacology and Toxicology. J. Pharm. Pharmacol. 2019, 71, 1353–1369. [Google Scholar] [CrossRef]
- Ming, X.; Yin, M.; Liyan, W. Antibacterial and Anti-Inflammatory Potential of Chinese Medicinal Herbs: Lonicerae flos, Lonicerae Japonicae flos, Scutellaria baicalensis Georgi, and Forsythia suspensa. Nat. Prod. Commun. 2022, 17, 1934578X221136673. [Google Scholar] [CrossRef]
- Liu, Y.; Gao, Z.; Zhao, Y.; Kong, L.; Ji, X.; Wu, J.; Gao, Z. Exploring Bioactive Constituents and Pharmacological Effects of Scutellaria baicalensis Georgi: A Review. Nat. Prod. Commun. 2024, 19, 1934578X241266692. [Google Scholar] [CrossRef]
- Kartesz, J.T. The Biota of North America Program (BONAP). Available online: https://bonap.net/MapGallery/County/Scutellaria angustifolia.png (accessed on 24 November 2025).
- Olmstead, R.G. Biological and Historical Factors Influencing Genetic Diversity in the Scutellaria angustifolia Complex (Labiatae). Evolution 1990, 44, 54–70. [Google Scholar] [CrossRef]
- Turner, M.; Gustafson, P. Wildflowers of the Pacific Northwest; Timber Press, Inc.: Portland, OR, USA, 2006; ISBN 978-0-88192-745-0. [Google Scholar]
- eFloras.org. Scutellaria baicalensis Georgi. Available online: http://www.efloras.org/florataxon.aspx?flora_id=2&taxon_id=200020285 (accessed on 24 November 2025).
- Zhao, Q.; Chen, X.Y.; Martin, C. Scutellaria baicalensis, the Golden Herb from the Garden of Chinese Medicinal Plants. Sci. Bull. 2016, 61, 1391–1398. [Google Scholar] [CrossRef]
- Wang, Z.-L.; Wang, S.; Kuang, Y.; Hu, Z.-M.; Qiao, X.; Ye, M. A Comprehensive Review on Phytochemistry, Pharmacology, and Flavonoid Biosynthesis of Scutellaria baicalensis. Pharm. Biol. 2018, 56, 465–484. [Google Scholar] [CrossRef]
- Song, J.-W.; Long, J.-Y.; Xie, L.; Zhang, L.-L.; Xie, Q.-X.; Chen, H.-J.; Deng, M.; Li, X.-F. Applications, Phytochemistry, Pharmacological Effects, Pharmacokinetics, Toxicity of Scutellaria baicalensis Georgi. and Its Probably Potential Therapeutic Effects on COVID-19: A Review. Chin. Med. 2020, 15, 102. [Google Scholar] [CrossRef]
- Lawson, S.K.; Satyal, P.; Setzer, W.N. Phytochemical Analysis of the Essential Oils from Aerial Parts of Four Scutellaria “Skullcap” Species Cultivated in South Alabama: Scutellaria baicalensis Georgi, S. Barbata D. Don, S. Incana Biehler, and S. Lateriflora L. Nat. Prod. Commun. 2021, 16, 1934578x211025930. [Google Scholar] [CrossRef]
- Wang, X.-D.; Shi, S.-L.; Ma, Y.-Z. Determination of Essential Oil from Scutellaria baicalensis Georgi (Lamiaceae) by GC-MS, and Assessment of Its Insecticidal Properties. Trop. J. Pharm. Res. 2022, 21, 1249–1254. [Google Scholar] [CrossRef]
- Takeoka, G.R.; Rodriguez, D.M.; Dao, L.; Patterson, R. Headspace Volatiles of Scutellaria baicalensis Georgi Flowers. J. Essent. Oil-Bearing Plants 2009, 12, 435–442. [Google Scholar] [CrossRef]
- eFloras.org. Scutellaria barbata D. Don. Available online: http://www.efloras.org/florataxon.aspx?flora_id=2&taxon_id=200020287 (accessed on 24 November 2025).
- Tao, G.; Balunas, M.J. Current Therapeutic Role and Medicinal Potential of Scutellaria barbata in Traditional Chinese Medicine and Western Research. J. Ethnopharmacol. 2016, 182, 170–180. [Google Scholar] [CrossRef]
- Wang, P.; Geng, X.; Pi, G.; Shi, R.; Yuan, Z.; Wang, C. Preparative Separation of Four Individual Flavonoids in Scutellaria barbata D. Don Based on High Selectivity Polymeric Adsorbents with Different Polarities. J. Chromatogr. B 2010, 878, 3375–3381. [Google Scholar] [CrossRef]
- Gong, T.; Wang, C.-F.; Yuan, J.-R.; Li, Y.; Gu, J.-F.; Zhao, B.-J.; Zhang, L.; Jia, X.-B.; Feng, L.; Liu, S.-L. Inhibition of Tumor Growth and Immunomodulatory Effects of Flavonoids and Scutebarbatines of Scutellaria barbata D. Don in Lewis-Bearing C57BL/6 Mice. Evid.-Based Complement. Altern. Med. 2015, 2015, 630760. [Google Scholar] [CrossRef]
- Yang, Y.-C.; Wang, C.-S.; Wei, M.-C. Separation and Quantification of Bioactive Flavonoids from Scutellaria barbata Using a Green Procedure. Food Bioprod. Process. 2019, 118, 77–90. [Google Scholar] [CrossRef]
- Dai, S.-J.; Tao, J.-Y.; Liu, K.; Jiang, Y.-T.; Shen, L. Neo-Clerodane Diterpenoids from Scutellaria barbata with Cytotoxic Activities. Phytochemistry 2006, 67, 1326–1330. [Google Scholar] [CrossRef]
- Dai, S.-J.; Sun, J.-Y.; Ren, Y.; Liu, K.; Shen, L. Bioactive ent-Clerodane Diterpenoids from Scutellaria barbata. Planta Med. 2007, 73, 1217–1220. [Google Scholar] [CrossRef] [PubMed]
- Zhu, F.; Di, Y.-T.; Liu, L.-L.; Zhang, Q.; Fang, X.; Yang, T.-Q.; Hao, X.-J.; He, H.-P. Cytotoxic Neoclerodane Diterpenoids from Scutellaria barbata. J. Nat. Prod. 2010, 73, 233–236. [Google Scholar] [CrossRef]
- Yuan, Q.-Q.; Song, W.-B.; Wang, W.-Q.; Xuan, L.-J. Scubatines A–F, New Cytotoxic Neo-Clerodane Diterpenoids from Scutellaria barbata D. Don. Fitoterapia 2017, 119, 40–44. [Google Scholar] [CrossRef]
- Yang, G.-C.; Liang, C.; Li, S.-G.; Liu, M.; Jia, M.-J.; Xu, X.-N.; Wang, X.-B.; Hua, H.-M.; Sun, L.-X. Neoclerodane Diterpenoids from Aerial Parts of Scutellaria barbata. Phytochem. Lett. 2017, 19, 1–6. [Google Scholar] [CrossRef]
- Li, S.T.; Xu, D.; Jia, J.; Zou, W.; Liu, J.Y.; Wang, Y.; Zhang, K.; Zheng, X.; Ma, Y.-Y.; Zhang, X.; et al. Structure and Anti-Inflammatory Activity of Neo-Clerodane Diterpenoids from Scutellaria barbata. Phytochemistry 2023, 213, 113771. [Google Scholar] [CrossRef]
- Yang, X.; Yang, Y.; Tang, S.; Tang, H.; Yang, G.; Xu, Q.; Wu, J. Anti-Tumor Effect of Polysaccharides from Scutellaria barbata D. Don on the 95-D Xenograft Model via Inhibition of the C-Met Pathway. J. Pharmacol. Sci. 2014, 125, 255–263. [Google Scholar] [CrossRef]
- Sun, P.; Sun, D.; Wang, X. Effects of Scutellaria barbata Polysaccharide on the Proliferation, Apoptosis and EMT of Human Colon Cancer HT29 Cells. Carbohydr. Polym. 2017, 167, 90–96. [Google Scholar] [CrossRef] [PubMed]
- Wang, T.-S.; Wang, S.-Q.; Xiao, D.-L. A Review of Phytochemistry and Antitumor Activity of a Valuable Medicinal Species: Scutellaria barbata. J. Med. Plants Res. 2012, 6, 4259–4275. [Google Scholar] [CrossRef]
- Chen, Q.; Rahman, K.; Wang, S.-J.; Zhou, S.; Zhang, H. Scutellaria barbata: A Review of Chemical Constituents, Pharmacological Activities and Clinical Application. Curr. Pharm. Des. 2020, 26, 160–175. [Google Scholar] [CrossRef] [PubMed]
- Sun, J.; Cao, Y.; Liu, Q.; Zhou, Z.; Xu, Y.; Liu, C. Chemical Constituents, Anti-Tumor Mechanisms, and Clinical Application: A Comprehensive Review on Scutellaria barbata. Molecules 2024, 29, 4143. [Google Scholar] [CrossRef]
- Yu, J.; Lei, J.; Yu, H.; Cai, X.; Zou, G. Chemical Composition and Antimicrobial Activity of the Essential Oil of Scutellaria barbata. Phytochemistry 2004, 65, 881–884. [Google Scholar] [CrossRef]
- Pan, R.; Guo, F.; Lu, H.; Feng, W.; Liang, Y. Development of the Chromatographic Fingerprint of Scutellaria barbata D. Don by GC-MS Combined with Chemometrics Methods. J. Pharm. Biomed. Anal. 2011, 55, 391–396. [Google Scholar] [CrossRef]
- Hamel, P.B.; Chiltoskey, M.U. Cherokee Plants and Their Uses—A 400 Year History; Herald Publishing Company: Sylva, NC, USA, 1975. [Google Scholar]
- Setzer, W.N. The Phytochemistry of Cherokee Aromatic Medicinal Plants. Medicines 2018, 5, 121. [Google Scholar] [CrossRef]
- Brock, C.; Whitehouse, J.; Tewfik, I.; Towell, T. The Use of Scutellaria lateriflora: A Pilot Survey amongst Herbal Medicine Practitioners. J. Herb. Med. 2012, 2, 34–41. [Google Scholar] [CrossRef]
- Sherman, S.H.; Joshee, N. Current Status of Research on the Medicinal Plant American Skullcap (Scutellaria lateriflora): A Review. J. Med. Act. Plants 2022, 11, 22–38. [Google Scholar]
- Gafner, S.; Bergeron, C.; Batcha, L.L.; Reich, J.; Arnason, J.T.; Burdette, J.E.; Pezzuto, J.M.; Angerhofer, C.K. Inhibition of [3H]-LSD Binding to 5-HT7 Receptors by Flavonoids from Scutellaria lateriflora. J. Nat. Prod. 2003, 66, 535–537. [Google Scholar] [CrossRef]
- Awad, R.; Arnason, J.T.; Trudeau, V.; Bergeron, C.; Budzinski, J.W.; Foster, B.C.; Merali, Z. Phytochemical and Biological Analysis of Skullcap (Scutellaria lateriflora L.): A Medicinal Plant with Anxiolytic Properties. Phytomedicine 2003, 10, 640–649. [Google Scholar] [CrossRef] [PubMed]
- Bergeron, C.; Gafner, S.; Clausen, E.; Carrier, D.J. Comparison of the Chemical Composition of Extracts from Scutellaria lateriflora Using Accelerated Solvent Extraction and Supercritical Fluid Extraction versus Standard Hot Water or 70% Ethanol Extraction. J. Agric. Food Chem. 2005, 53, 3076–3080. [Google Scholar] [CrossRef]
- Zhang, Z.; Lian, X.Y.; Li, S.; Stringer, J.L. Characterization of Chemical Ingredients and Anticonvulsant Activity of American Skullcap (Scutellaria lateriflora). Phytomedicine 2009, 16, 485–493. [Google Scholar] [CrossRef]
- Bruno, M.; Cruciata, M.; Bondi, M.L.; Piozzi, F.; de la Torre, M.; Rodgriguez, B.; Servettaz, O. Neo-Clerodane Diterpenoids from Scutellaria lateriflora. Phytochemistry 1998, 48, 687–691. [Google Scholar] [CrossRef]
- van den Dool, H.; Kratz, P.D. A Generalization of the Retention Index System Including Linear Temperature Programmed Gas-Liquid Partition Chromatography. J. Chromatogr. A 1963, 11, 463–471. [Google Scholar] [CrossRef]
- Adams, R.P. Identification of Essential Oil Components by Gas Chromatography/Mass Spectrometry, 4th ed.; Allured Publishing: Carol Stream, IL, USA, 2007; ISBN 978-1-932633-21-4. [Google Scholar]
- Satyal, P. Development of GC-MS Database of Essential Oil Components by the Analysis of Natural Essential Oils and Synthetic Compounds and Discovery of Biologically Active Novel Chemotypes in Essential Oils. Ph.D. Dissertation, University of Alabama in Huntsville, Huntsville, AL, USA, 2015. [Google Scholar]
- Mondello, L. FFNSC 3; Shimadzu Scientific Instruments: Columbia, MD, USA, 2016. [Google Scholar]
- NIST20; National Institute of Standards and Technology: Gaithersburg, MD, USA, 2020.
- Fukuhara, K.; Fujimori, T.; Shigematsu, H.; Ohnishi, A. Essential Oil of Scutellaria baicalensis G. Agric. Biol. Chem. 1987, 51, 1449–1451. [Google Scholar] [CrossRef]
- Schmidt, J.M.; Noletto, J.A.; Vogler, B.; Setzer, W.N. Abaco Bush Medicine: Chemical Composition of the Essential Oils of Four Aromatic Medicinal Plants from Abaco Island, Bahamas. J. Herbs Spices Med. Plants 2006, 12, 43–65. [Google Scholar] [CrossRef]
- da Silva, S.L.; Figueiredo, P.M.; Yano, T. Cytotoxic Evaluation of Essential Oil from Zanthoxylum rhoifolium Lam. Leaves. Acta Amaz. 2007, 37, 281–286. [Google Scholar] [CrossRef]
- Dahham, S.S.; Tabana, Y.M.; Iqbal, M.A.; Ahamed, M.B.K.; Ezzat, M.O.; Majid, A.S.A.; Majid, A.M.S.A. The Anticancer, Antioxidant and Antimicrobial Properties of the Sesquiterpene β-Caryophyllene from the Essential Oil of Aquilaria crassna. Molecules 2015, 20, 11808–11829. [Google Scholar] [CrossRef]
- Su, Y.-C.; Ho, C.-L. Composition of the Leaf Essential Oil of Phoebe formosana from Taiwan and Its in Vitro Cytotoxic, Antibacterial, and Antifungal Activities. Nat. Prod. Commun. 2016, 11, 845–848. [Google Scholar] [CrossRef]
- Schmidt, E.; Bail, S.; Friedl, S.M.; Jirovetz, L.; Buchbauer, G.; Wanner, J.; Denkova, Z.; Slavchev, A.; Stoyanova, A.; Geissler, M. Antimicrobial Activities of Single Aroma Compounds. Nat. Prod. Commun. 2010, 5, 1365–1368. [Google Scholar] [CrossRef] [PubMed]
- Rather, M.A.; Dar, B.A.; Dar, M.Y.; Wani, B.A.; Shah, W.A.; Bhat, B.A.; Ganai, B.A.; Bhat, K.A.; Anand, R.; Qurishi, M.A. Chemical Composition, Antioxidant and Antibacterial Activities of the Leaf Essential Oil of Juglans regia L. and Its Constituents. Phytomedicine 2012, 19, 1185–1190. [Google Scholar] [CrossRef]
- Pacheco-Hernández, Y.; Sánchez-Hernández, G.R.; Reyes-Cervantes, E.; Romero-Arenas, O.; Pérez-Xochipa, I.; Villa-Ruano, N. Chemical Variation and Pharmacological Properties of Dyssodia decipiens Essential Oil. Chem. Biodivers. 2020, 17, e2000487. [Google Scholar] [CrossRef]
- Tambe, Y.; Tsujiuchi, H.; Honda, G.; Ikeshiro, Y.; Tanaka, S. Gastric Cytoprotection of the Non-Steroidal Anti-Inflammatory Sesquiterpene, β-Caryophyllene. Planta Med. 1996, 62, 469–470. [Google Scholar] [CrossRef]
- Fernandes, E.S.; Passos, G.F.; Medeiros, R.; da Cunha, F.M.; Ferreira, J.; Campos, M.M.; Pianowski, L.F.; Calixto, J.B. Anti-Inflammatory Effects of Compounds Alpha-Humulene and (−)-trans-Caryophyllene Isolated from the Essential Oil of Cordia verbenacea. Eur. J. Pharmacol. 2007, 569, 228–236. [Google Scholar] [CrossRef] [PubMed]
- Bakir, B.; Him, A.; Özbek, H.; Düz, E.; Tütüncü, M. Investigation of the Anti-Inflammatory and Analgesic Activities of β-Caryophyllene. Int. J. Essent. Oil Ther. 2008, 2, 41–44. [Google Scholar]
- Klauke, A.; Racz, I.; Pradier, B.; Markert, A.; Zimmer, A.M.; Gertsch, J.; Zimmer, A. The Cannabinoid CB2 Receptor-Selective Phytocannabinoid β-Caryophyllene Exerts Analgesic Effects in Mouse Models of Inflammatory and Neuropathic Pain. Eur. Neuropsychopharmacol. 2014, 24, 608–620. [Google Scholar] [CrossRef]
- Ceccarelli, I.; Fiorenzani, P.; Pessina, F.; Pinassi, J.; Aglianò, M.; Miragliotta, V.; Aloisi, A.M. The CB2 Agonist β-Caryophyllene in Male and Female Rats Exposed to a Model of Persistent Inflammatory Pain. Front. Neurosci. 2020, 14, 850. [Google Scholar] [CrossRef]
- Badalamenti, N.; Ilardi, V.; Bruno, M.; Maggi, F.; Quassinti, L.; Bramucci, M. Cladanthus scariosus Essential Oil and Its Principal Constituents with Cytotoxic Effects on Human Tumor Cell Lines. Plants 2024, 13, 1555. [Google Scholar] [CrossRef]
- Dosoky, N.S.; Kirpotina, L.N.; Schepetkin, I.A.; Khlebnikov, A.I.; Lisonbee, B.L.; Black, J.L.; Woolf, H.; Thurgood, T.L.; Graf, B.L.; Satyal, P.; et al. Volatile Composition, Antimicrobial Activity, and in Vitro Innate Immunomodulatory Activity of Echinacea purpurea (L.) Moench Essential Oils. Molecules 2023, 28, 7330. [Google Scholar] [CrossRef]
- Sangwan, N.S.; Farooqi, A.H.A.; Shabih, F.; Sangwan, R.S. Regulation of Essential Oil Production in Plants. Plant Growth Regul. 2001, 34, 3–21. [Google Scholar] [CrossRef]
- Barra, A. Factors Affecting Chemical Variability of Essential Oils: A Review of Recent Developments. Nat. Prod. Commun. 2009, 4, 1147–1154. [Google Scholar] [CrossRef]
- Moore, B.D.; Andrew, R.L.; Külheim, C.; Foley, W.J. Explaining Intraspecific Diversity in Plant Secondary Metabolites in an Ecological Context. New Phytol. 2014, 201, 733–750. [Google Scholar] [CrossRef] [PubMed]
- Etri, K.; Pluhár, Z. Exploring Chemical Variability in the Essential Oils of the Thymus Genus. Plants 2024, 4, 1375. [Google Scholar] [CrossRef]
- Hassiotis, C.N.; Vlachonasios, K.E. How Biological and Environmental Factors Affect the Quality of Lavender Essential Oils. Physiologia 2025, 5, 11. [Google Scholar] [CrossRef]
- Vieira, R.F.; Grayer, J.; Paton, A.; Simon, J.E. Genetic Diversity of Ocimum gratissimum L. Based on Volatile Oil Constituents, Flavonoids and RAPD Markers. Biochem. Syst. Ecol. 2001, 29, 287–304. [Google Scholar] [CrossRef] [PubMed]
- Xavier, J.K.A.M.; Baia, T.G.C.; Alegria, O.V.C.; Figueiredo, P.L.B.; Carneiro, A.R.; Moreira, E.C.d.O.; Maia, J.G.S.; Setzer, W.N.; da Silva, J.K.R. Essential Oil Chemotypes and Genetic Variability of Cinnamomum verum Leaf Samples Commercialized and Cultivated in the Amazon. Molecules 2022, 27, 7337. [Google Scholar] [CrossRef]
- Çelik, C.; Tuğlu, Ü.; Telci, İ.; Karakurt, Y.; Özek, T.; Özek, G. Determination of Gene Expression of Some Essential Oil Components and Some Genes Responsible for the Synthesis of These Components in Mentha Species Harvested in Different Phenological Periods. Acta Physiol. Plant. 2025, 47, 68. [Google Scholar] [CrossRef]
- Lakušić, D.V.; Ristić, M.S.; Slavkovska, V.N.; Šinžar-Sekulić, J.B.; Lakušić, B.S. Environment-Related Variations of the Composition of the Essential Oils of Rosemary (Rosmarinus officinalis L.) in the Balkan Penninsula. Chem. Biodivers. 2012, 9, 1286–1302. [Google Scholar] [CrossRef] [PubMed]
- Mansinhos, I.; Gonçalves, S.; Rodríguez-Solana, R.; Moreno-Rojas, J.M.; Romano, A. Environmental Factors Related to Climate Change Alter the Chemical Composition and Biological Activity of Lavandula viridis L’Hér Essential Oil. Agriculture 2024, 14, 1067. [Google Scholar] [CrossRef]
- de Sousa Peixoto Barros, L.; de Nazaré Santos da Cruz, E.; de Araújo Guimarães, B.; Setzer, W.N.; Veras Mourão, R.H.; do Rosário da Silva, J.K.; Silva da Costa, J.; Baia Figueiredo, P.L. Chemometric Analysis of the Seasonal Variation in the Essential Oil Composition and Antioxidant Activity of a New Geraniol Chemotype of Lippia alba (Mill.) N.E.Br. ex Britton & P. Wilson from the Brazilian Amazon. Biochem. Syst. Ecol. 2022, 105, 104503. [Google Scholar] [CrossRef]
- Palermo, T.B.; Cappellari, L.d.R.; Palermo, J.S.; Giordano, W.; Banchio, E. Simultaneous Impact of Rhizobacteria Inoculation and Leaf-Chewing Insect Herbivory on Essential Oil Production and VOC Emissions in Ocimum basilicum. Plants 2024, 13, 932. [Google Scholar] [CrossRef] [PubMed]
- de Brito-Machado, D.; Ramos, Y.J.; Defaveri, A.C.A.e.; de Queiroz, G.A.; Guimarães, E.F.; de Lima Moreira, D. Volatile Chemical Variation of Essential Oils and Their Correlation with Insects, Phenology, Ontogeny and Microclimate: Piper mollicomum Kunth, a Case of Study. Plants 2022, 11, 3535. [Google Scholar] [CrossRef]
- Giannoulis, K.D.; Evangelopoulos, V.; Gougoulias, N.; Wogiatzi, E. Could Bio-Stimulators Affect Flower, Essential Oil Yield, and Its Composition in Organic Lavender (Lavandula angustifolia) Cultivation? Ind. Crop. Prod. 2020, 154, 112611. [Google Scholar] [CrossRef]
- Grant, A.J.; Dickens, J.C. Functional Characterization of the Octenol Receptor Neuron on the Maxillary Palps of the Yellow Fever Mosquito, Aedes aegypti. PLoS ONE 2011, 6, e21785. [Google Scholar] [CrossRef]
- Cilek, J.E.; Ikediobi, C.O.; Hallmon, C.F.; Johnson, R.; Okungbowa, O.; Onyeozili, E.N.; Khalil, L.M.; Ayuk-Takem, L.; Latinwo, L.M.; Bernier, U.R. Evaluation of Several Novel Alkynols, Alkenols, and Selected Host Odor Blends as Attractants to Female Aedes albopictus and Culex quinquefasciatus. J. Am. Mosq. Control Assoc. 2012, 28, 199–205. [Google Scholar] [CrossRef]
- Vale, G.A.; Hall, D.R. The Role of 1-Octen-3-Ol, Acetone and Carbon Dioxide in the Attraction of Tsetse Flies, Glossina spp. (Diptera: Glossinidae), to Ox Odour. Bull. Entomol. Res. 1985, 75, 209–218. [Google Scholar] [CrossRef]
- Carr, A.L.; Roe, R.M.; Arellano, C.; Sonenshine, D.E.; Schal, C.; Apperson, C.S. Responses of Amblyomma americanum and Dermacentor variabilis to Odorants That Attract Haematophagous Insects. Med. Vet. Entomol. 2013, 27, 86–95. [Google Scholar] [CrossRef]
- Likens, S.T.; Nickerson, G.B. Detection of Certain Hop Oil Constituents in Brewing Products. Proc. Annu. Meet. Am. Soc. Brew. Chem. 1964, 22, 5–13. [Google Scholar] [CrossRef]
- Augusto, F.; Leite e Lopes, A.; Zini, C.A. Sampling and Sample Preparation for Analysis of Aromas and Fragrances. Trends Anal. Chem. 2003, 22, 160–169. [Google Scholar] [CrossRef]
- Au-Yeung, C.Y.; MacLeod, A.J. A Comparison of the Efficiency of the Likens and Nickerson Extractor for Aqueous, Lipid/Aqueous, and Lipid Samples. J. Agric. Food Chem. 1981, 29, 502–505. [Google Scholar] [CrossRef]
- Satyal, P.; Dosoky, N.S.; Poudel, A.; Swor, K.; Setzer, W.N. Chemical Composition of the Aerial Parts Essential Oil of Chrysothamnus viscidiflorus from Southwestern Idaho. J. Essent. Oil Plant Compos. 2023, 1, 115–121. [Google Scholar] [CrossRef]

| Species | Narrowleaf Skullcap (Scutellaria angustifolia) | Blue Skullcap (Scutellaria lateriflora) | Baikal Skullcap (Scutellaria baicalensis) | Barbat Skullcap (Scutellaria barbata) |
|---|---|---|---|---|
| Naturally occurring region | North America intermountain west (dry slopes) | North America (wetlands) | Siberia, Mongolia, N. China (dry slopes) | South–Central and SE China (moist fields) |
| Plant structure and growth habit | Perennial growing from prolific underground stolons, thin square stems with little or no branching, 5 to 20 cm tall, ours 10–30 cm | Perennial growing from underground stolons, square stems with prolific branching, 60–90 cm tall, ours 60–100 cm | Perennial with square stems growing from a woody base, stems with prolific branching, 20 to 30 cm tall, ours 50–80 cm | Perennial with thin square stems growing from a compact base, many stems with little or no branching, 20 to 30 cm tall, ours 50–75 cm |
| Leaves | Smooth lanceolate to ovate leaves, ours to 21 by 10 mm | Thin, ovate, lightly toothed leaves, ours to 61 by 29 mm | Narrow, lance-shaped leaves, ours to 42 by 11 mm | Triangular–lanceolate leaves, ours to 32 by 17 mm |
| Flowers | Intense blue, violet, or blue and white flowers, 14–27 mm by 6–11 mm | Small blue–violet or light blue flowers borne on one-sided racemes, 7 mm by 2.5–3 mm | Purple–violet or intense blue in upright spikes, ours 22–25 by 9–11 mm | Violet–blue to light blue, tiny, paired blooms, 14–15 mm by 5 mm |
| Plant part used medicinally | Leaves and stems by herbalists | Leaves and stems by herbalists | Root in traditional Chinese medicine | Whole plant or aerial parts in traditional Chinese medicine |
| RIcalc | RIdb | Compounds | #1 | #2 | #3 | #4 | #5 | #6 | #7 | #8 | #10 | #11 | #11 (8-8) | #12 | #12 (8-8) |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 850 | 850 | (2E)-Hexenal | 1.3 | 0.2 | 3.1 | 3.3 | 2.0 | 0.6 | 1.3 | 6.2 | 1.1 | 2.8 | 3.2 | 1.1 | 2.5 |
| 962 | 960 | Benzaldehyde | 1.8 | 0.2 | 2.2 | 2.3 | 2.2 | - | 0.9 | 1.6 | 0.8 | 0.6 | 1.7 | 0.8 | 1.3 |
| 977 | 974 | 1-Octen-3-ol | 2.3 | 0.6 | 6.0 | 2.1 | 1.7 | 0.1 | - | 3.5 | 0.1 | 1.6 | 5.3 | 1.1 | 2.8 |
| 1097 | 1099 | Linalool | 1.1 | 3.1 | 0.9 | 4.9 | 3.6 | 4.3 | 2.2 | 1.2 | 2.1 | 6.8 | 1.7 | 7.7 | 0.7 |
| 1359 | 1356 | (E)-Benzalacetone | 2.6 | 0.2 | 3.9 | 4.2 | 3.7 | - | 2.8 | 1.2 | - | 0.8 | 2.8 | 1.1 | 1.6 |
| 1374 | 1377 | α-Copaene | 1.2 | 1.3 | 1.2 | 1.4 | 1.4 | 2.6 | 1.9 | 1.3 | 2.1 | 1.8 | 1.1 | 1.5 | 1.5 |
| 1382 | 1382 | β-Bourbonene | 2.8 | 3.1 | 3.2 | 4.0 | 4.3 | 6.1 | 4.5 | 5.4 | 9.4 | 4.9 | 3.4 | 4.4 | 4.5 |
| 1389 | 1390 | trans-β-Elemene | 1.1 | 1.1 | 1.1 | 1.2 | 1.3 | 1.8 | 1.5 | 0.9 | 1.8 | 1.7 | 1.1 | 1.8 | 2.3 |
| 1420 | 1417 | (E)-β-Caryophyllene | 25.0 | 29.2 | 18.1 | 11.7 | 12.8 | 15.6 | 10.5 | 21.6 | 12.4 | 7.6 | 18.1 | 8.1 | 4.9 |
| 1430 | 1433 | β-Copaene | 1.1 | 0.7 | 0.8 | 0.9 | 1.0 | 1.2 | 1.2 | 1.0 | 2.2 | 1.1 | 0.9 | 1.0 | 1.1 |
| 1452 | 1452 | (E)-β-Farnesene | 1.5 | 1.2 | 1.5 | 1.4 | 1.6 | - | 1.6 | 0.8 | 0.9 | 1.8 | 1.1 | 2.0 | 0.5 |
| 1456 | 1454 | α-Humulene | 2.6 | 2.9 | 2.1 | 1.7 | 1.9 | 2.1 | 1.6 | 2.0 | 1.8 | 1.8 | 2.0 | 1.9 | 1.2 |
| 1481 | 1480 | Germacrene D | 32.5 | 41.6 | 40.5 | 42.9 | 46.5 | 48.8 | 43.8 | 29.4 | 38.9 | 48.8 | 37.2 | 50.1 | 58.3 |
| 1517 | 1518 | δ-Cadinene | 1.7 | 1.3 | 1.4 | 1.6 | 1.3 | 1.6 | 3.1 | 1.3 | 2.3 | 1.5 | 2.1 | 1.6 | 1.2 |
| 1658 | 1655 | α-Cadinol | 0.8 | 2.4 | 1.9 | 2.2 | 2.0 | 3.2 | 4.1 | 1.7 | 4.3 | 3.0 | 3.6 | 2.5 | 1.4 |
| RIcalc | RIdb | Compound | S. baicalensis | S. barbata | S. lateriflora |
|---|---|---|---|---|---|
| 802 | 801 | Hexanal | 0.1 | 2.0 | 1.3 |
| 849 | 849 | (2E)-Hexenal | 0.6 | 5.1 | 3.4 |
| 851 | 853 | (3Z)-Hexenol | 0.5 | 4.4 | 1.6 |
| 961 | 960 | Benzaldehyde | - | - | 7.5 |
| 978 | 978 | 1-Octen-3-ol | 22.3 | 59.9 | 28.3 |
| 995 | 996 | 3-Octanol | 2.4 | 3.1 | - |
| 1030 | 1030 | Limonene | tr | - | 6.0 |
| 1031 | 1031 | β-Phellandrene | - | - | 5.1 |
| 1044 | 1043 | Phenylacetaldehyde | 0.2 | 1.3 | 0.7 |
| 1044 | 1044 | Salicylaldehyde | - | - | 1.0 |
| 1064 | 1064 | Acetophenone | 0.1 | - | 24.8 |
| 1098 | 1099 | Linalool | 4.1 | 9.5 | 1.2 |
| 1286 | 1290 | o-Acetanisole | - | - | 2.8 |
| 1356 | 1356 | (E)-Benzalacetone | - | - | 5.9 |
| 1382 | 1382 | β-Bourbonene | 2.0 | 1.2 | - |
| 1420 | 1417 | (E)-β-Caryophyllene | 22.3 | 3.1 | 3.9 |
| 1455 | 1454 | α-Humulene | 2.0 | 0.5 | 1.4 |
| 1481 | 1480 | Germacrene D | 28.3 | 4.8 | - |
| 1496 | 1497 | Bicyclogermacrene | 3.2 | 0.7 | - |
| 1518 | 1518 | δ-Cadinene | 1.2 | 0.5 | - |
| 1645 | 1645 | τ-Muurolol | 1.8 | - | - |
| 2144 | 2143 | Serratol | - | 1.2 | - |
| 2500 | 2500 | Pentacosane | - | 1.4 | - |
| Compounds | Collection Site | ||||
|---|---|---|---|---|---|
| Hubei, China a | Hunan, China b | Newville, Alabama c | Ontario, Oregon d | ||
| 1-Octen-3-ol | 7.1 | 6.2 | 25.6 | 20.1 | 59.9 |
| Linalool | 6.7 | 5.8 | 3.3 | 2.5 | 9.5 |
| Menthol | 7.7 | - | - | - | - |
| Thymol | 1.4 | - | 2.2 | 7.7 | - |
| Carvacrol | - | - | 2.3 | 8.9 | - |
| Methyl eugenol | 5.6 | 1.2 | - | - | - |
| (E)-β-Caryophyllene | - | 4.4 | 3.6 | 2.7 | 3.1 |
| (Z)-α-trans-Bergamotol | 5.1 | - | - | - | - |
| Phytone | 11.0 | 4.6 | - | 0.6 | - |
| 1-Heptadecanol | 5.0 | - | - | - | - |
| Palmitic acid | - | 28.6 | 15.6 | 13.0 | - |
| Phytol | 7.8 | - | 1.8 | 2.3 | - |
| Selection Number | Collection Site | Collection Date | Coordinates | Elevation (m) | |
|---|---|---|---|---|---|
| North | West | ||||
| 1 | West of Riggins, Idaho | 26 June 2018 | 45.552 | 116.404 | 1585 |
| 2 | Reynolds Creek, Idaho | 18 April 2020 | 43.260 | 116.784 | 1225 |
| 3 | Brogan Hill, Oregon | 21 April 2020 | 44.264 | 117.624 | 1041 |
| 4 | Cow Creek, Lucille, Idaho | 26 April 2020 | 45.628 | 116.411 | 1929 |
| 5 | Mitchell, Oregon | 17 May 2020 | 44.561 | 120.059 | 1292 |
| 6 | Fairview Campground, Oregon | 18 May 2020 | 44.955 | 119.715 | 1289 |
| 7 | Grids Creek, Oregon | 18 May 2020 | 44.708 | 120.172 | 623 |
| 8 | Austin, Oregon | 19 May 2020 | 44.586 | 118.442 | 1298 |
| 9 | Twinkenham, Oregon | 31 May 2020 | 44.824 | 120.153 | 764 |
| 10 | Fossil, Oregon | 31 May 2020 | 44.895 | 120.108 | 1079 |
| 11 | Clarno, Oregon | 1 June 2020 | 44.901 | 120.324 | 648 |
| 12 | Southwest of McCall, Idaho | 21 May 2021 | 44.953 | 116.186 | 1566 |
| Sample | Mass Plant Material (g) | Mass Essential Oil (g) | % Yield | Color |
|---|---|---|---|---|
| Scutellaria angustifolia #1 | 61.55 | 4.0438 | 6.570 | pale yellow |
| Scutellaria angustifolia #2 | 109.96 | 6.0023 | 5.459 | pale yellow |
| Scutellaria angustifolia #3 | 65.55 | 4.2037 | 6.413 | pale yellow |
| Scutellaria angustifolia #4 | 53.46 | 3.4358 | 6.427 | yellow |
| Scutellaria angustifolia #5 | 48.33 | 4.2186 | 8.729 | pale yellow |
| Scutellaria angustifolia #6 | 46.75 | 3.7603 | 8.043 | pale yellow |
| Scutellaria angustifolia #7 | 61.55 | 4.2111 | 6.842 | pale yellow |
| Scutellaria angustifolia #8 | 75.76 | 5.0012 | 6.601 | pale yellow |
| Scutellaria angustifolia #10 | 46.54 | 4.2695 | 9.174 | yellow |
| Scutellaria angustifolia #11 | 99.21 | 5.3532 | 5.396 | pale yellow |
| Scutellaria angustifolia #11 (8/8) | 69.59 | 4.1792 | 6.005 | colorless |
| Scutellaria angustifolia #12 | 73.63 | 4.5978 | 6.244 | pale yellow |
| Scutellaria angustifolia #12 (8/8) | 52.82 | 3.7001 | 7.005 | colorless |
| Scutellaria baicalensis | 84.33 | 5.6537 | 6.704 | colorless |
| Scutellaria barbata | 44.91 | 5.2731 | 11.741 | colorless |
| Scutellaria lateriflora | 93.65 | 5.1872 | 5.539 | pale yellow |
Disclaimer/Publisher’s Note: The statements, opinions and data contained in all publications are solely those of the individual author(s) and contributor(s) and not of MDPI and/or the editor(s). MDPI and/or the editor(s) disclaim responsibility for any injury to people or property resulting from any ideas, methods, instructions or products referred to in the content. |
© 2026 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.
Share and Cite
Shock, C.C.; Poudel, A.; Satyal, P.; Setzer, W.N. Chemical Compositions of Scutellaria Essential Oils Cultivated in Eastern Oregon: S. angustifolia, S. baicalensis, S. barbata, and S. lateriflora. Plants 2026, 15, 1075. https://doi.org/10.3390/plants15071075
Shock CC, Poudel A, Satyal P, Setzer WN. Chemical Compositions of Scutellaria Essential Oils Cultivated in Eastern Oregon: S. angustifolia, S. baicalensis, S. barbata, and S. lateriflora. Plants. 2026; 15(7):1075. https://doi.org/10.3390/plants15071075
Chicago/Turabian StyleShock, Clinton C., Ambika Poudel, Prabodh Satyal, and William N. Setzer. 2026. "Chemical Compositions of Scutellaria Essential Oils Cultivated in Eastern Oregon: S. angustifolia, S. baicalensis, S. barbata, and S. lateriflora" Plants 15, no. 7: 1075. https://doi.org/10.3390/plants15071075
APA StyleShock, C. C., Poudel, A., Satyal, P., & Setzer, W. N. (2026). Chemical Compositions of Scutellaria Essential Oils Cultivated in Eastern Oregon: S. angustifolia, S. baicalensis, S. barbata, and S. lateriflora. Plants, 15(7), 1075. https://doi.org/10.3390/plants15071075

