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Volume 14
Issue 17
10.3390/plants14172686
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This is an early access version, the complete PDF, HTML, and XML versions will be available soon.
Article

Genome-Wide Identification of the Glycosyl Hydrolase Family 1 Genes in Brassica napus L. and Functional Characterization of BnBGLU77

by
Xingzhi Wei
1,2,3,
Yunshan Tang
1,2,3,
Yuanyuan Liu
1,2,3,
Shulin Shen
1,2,3,
Jie Xu
1,2,3,
Lulu Chen
1,2,3,
Meifang Li
1,2,3,
Huiyan Zhao
1,2,3,
Ti Zhang
1,2,3,
Hai Du
1,2,3,
Huafang Wan
1,2,3,
Cunmin Qu
1,2,3,* and
Nengwen Yin
1,2,3,*
1
Integrative Science Center of Germplasm Creation in Western China (Chongqing) Science City, College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, China
2
Academy of Agricultural Sciences, Southwest University, Chongqing 400715, China
3
Engineering Research Center of South Upland Agriculture, Ministry of Education, Chongqing 400715, China
*
Authors to whom correspondence should be addressed.
Plants 2025, 14(17), 2686; https://doi.org/10.3390/plants14172686
Submission received: 10 June 2025 / Revised: 6 August 2025 / Accepted: 27 August 2025 / Published: 28 August 2025
(This article belongs to the Special Issue Molecular Genetics and Breeding of Oilseed Crops—2nd Edition)
Versions Notes

Abstract

The β-glucosidases (BGLUs) of Glycoside Hydrolase Family 1 (GH1) exhibit essential functions in plant secondary metabolism and stress responses, mediated by their dual catalytic capabilities in hydrolysis and transglycosylation. This study identified 149 BGLU family members within B. napus (Brassica napus L.), which were systematically categorized into 10 distinct subgroups. Subsequent characterization encompassed detailed examination of their motif composition, chromosomal distribution, gene collinearity, selection pressure, and expression profiling. Transient overexpression of BnBGLU77 in N. benthamiana (Nicotiana benthamiana), combined with untargeted metabolomics analysis, revealed pronounced modulatory effects on the degradation and accumulation of β-glucosidic compounds, suggesting potential roles of the protein encoded by BnBGLU77 in metabolic homeostasis and stress response mechanisms. These experimental results first validated the bidirectional catalytic activity of a BGLU enzyme in B. napus, while simultaneously advancing fundamental understanding of BnBGLU gene functions and providing new insights for developing stress-resistant rapeseed cultivars through targeted genetic improvement.
Keywords: glycosyl hydrolase family 1 genes; B. napus; genome-wide analysis; catalytic function; stress adaptation glycosyl hydrolase family 1 genes; B. napus; genome-wide analysis; catalytic function; stress adaptation

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MDPI and ACS Style

Wei, X.; Tang, Y.; Liu, Y.; Shen, S.; Xu, J.; Chen, L.; Li, M.; Zhao, H.; Zhang, T.; Du, H.; et al. Genome-Wide Identification of the Glycosyl Hydrolase Family 1 Genes in Brassica napus L. and Functional Characterization of BnBGLU77. Plants 2025, 14, 2686. https://doi.org/10.3390/plants14172686

AMA Style

Wei X, Tang Y, Liu Y, Shen S, Xu J, Chen L, Li M, Zhao H, Zhang T, Du H, et al. Genome-Wide Identification of the Glycosyl Hydrolase Family 1 Genes in Brassica napus L. and Functional Characterization of BnBGLU77. Plants. 2025; 14(17):2686. https://doi.org/10.3390/plants14172686

Chicago/Turabian Style

Wei, Xingzhi, Yunshan Tang, Yuanyuan Liu, Shulin Shen, Jie Xu, Lulu Chen, Meifang Li, Huiyan Zhao, Ti Zhang, Hai Du, and et al. 2025. "Genome-Wide Identification of the Glycosyl Hydrolase Family 1 Genes in Brassica napus L. and Functional Characterization of BnBGLU77" Plants 14, no. 17: 2686. https://doi.org/10.3390/plants14172686

APA Style

Wei, X., Tang, Y., Liu, Y., Shen, S., Xu, J., Chen, L., Li, M., Zhao, H., Zhang, T., Du, H., Wan, H., Qu, C., & Yin, N. (2025). Genome-Wide Identification of the Glycosyl Hydrolase Family 1 Genes in Brassica napus L. and Functional Characterization of BnBGLU77. Plants, 14(17), 2686. https://doi.org/10.3390/plants14172686

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