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Volume 12
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10.3390/plants12091763
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Article

Features of Photosynthesis in Arabidopsis thaliana Plants with Knocked Out Gene of Alpha Carbonic Anhydrase 2

by
Elena M. Nadeeva
,
Lyudmila K. Ignatova
,
Natalia N. Rudenko
,
Daria V. Vetoshkina
,
Ilya A. Naydov
,
Marina A. Kozuleva
and
Boris N. Ivanov
*
Institute of Basic Biological Problems, Federal Research Center “Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences”, 142290 Pushchino, Moscow Region, Russia
*
Author to whom correspondence should be addressed.
Plants 2023, 12(9), 1763; https://doi.org/10.3390/plants12091763
Submission received: 28 February 2023 / Revised: 14 April 2023 / Accepted: 18 April 2023 / Published: 25 April 2023
(This article belongs to the Special Issue Carbonic Anhydrases and Carbon Metabolism in Plants)
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Abstract

The knockout of the At2g28210 gene encoding α-carbonic anhydrase 2 (α-CA2) in Arabidopsis thaliana (Columbia) led to alterations in photosynthetic processes. The effective quantum yields of both photosystem II (PSII) and photosystem I (PSI) were higher in α-carbonic anhydrase 2 knockout plants (α-CA2-KO), and the reduction state of plastoquinone pool was lower than in wild type (WT). The electron transport rate in the isolated thylakoids measured with methyl viologen was higher in α-CA2-KO plants. The amounts of reaction centers of PSII and PSI were similar in WT and α-CA2-KO plants. The non-photochemical quenching of chlorophyll a fluorescence in α-CA2-KO leaves was lower at the beginning of illumination, but became slightly higher than in WT leaves when the steady state was achieved. The degree of state transitions in the leaves was lower in α-CA2-KO than in WT plants. Measurements of the electrochromic carotenoid absorbance shift (ECS) revealed that the light-dependent pH gradient (ΔpH) across the thylakoid membrane was lower in the leaves of α-CA2-KO plants than in WT plants. The starch content in α-CA2-KO leaves was lower than in WT plants. The expression levels of the genes encoding chloroplast CAs in α-CA2-KO changed noticeably, whereas the expression levels of genes of cytoplasmic CAs remained almost the same. It is proposed that α-CA2 may be situated in the chloroplasts.
Keywords: carbonic anhydrase; Arabidopsis thaliana; photosynthesis; thylakoid membrane; protons; non-photochemical quenching carbonic anhydrase; Arabidopsis thaliana; photosynthesis; thylakoid membrane; protons; non-photochemical quenching

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MDPI and ACS Style

Nadeeva, E.M.; Ignatova, L.K.; Rudenko, N.N.; Vetoshkina, D.V.; Naydov, I.A.; Kozuleva, M.A.; Ivanov, B.N. Features of Photosynthesis in Arabidopsis thaliana Plants with Knocked Out Gene of Alpha Carbonic Anhydrase 2. Plants 2023, 12, 1763. https://doi.org/10.3390/plants12091763

AMA Style

Nadeeva EM, Ignatova LK, Rudenko NN, Vetoshkina DV, Naydov IA, Kozuleva MA, Ivanov BN. Features of Photosynthesis in Arabidopsis thaliana Plants with Knocked Out Gene of Alpha Carbonic Anhydrase 2. Plants. 2023; 12(9):1763. https://doi.org/10.3390/plants12091763

Chicago/Turabian Style

Nadeeva, Elena M., Lyudmila K. Ignatova, Natalia N. Rudenko, Daria V. Vetoshkina, Ilya A. Naydov, Marina A. Kozuleva, and Boris N. Ivanov. 2023. "Features of Photosynthesis in Arabidopsis thaliana Plants with Knocked Out Gene of Alpha Carbonic Anhydrase 2" Plants 12, no. 9: 1763. https://doi.org/10.3390/plants12091763

APA Style

Nadeeva, E. M., Ignatova, L. K., Rudenko, N. N., Vetoshkina, D. V., Naydov, I. A., Kozuleva, M. A., & Ivanov, B. N. (2023). Features of Photosynthesis in Arabidopsis thaliana Plants with Knocked Out Gene of Alpha Carbonic Anhydrase 2. Plants, 12(9), 1763. https://doi.org/10.3390/plants12091763

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