Genetic Diversity and Population Structure of Common Bean (Phaseolus vulgaris L.) Landraces in the Lazio Region of Italy
Abstract
:1. Introduction
2. Material and Methods
2.1. Plant Material
2.2. Morpho-Phenotypic Seed Analysis
2.3. Growth Habit
2.4. Phaseolin (PHAS) and Phytohemagglutinin (PHA) Analysis
2.5. DNA Isolation and Microsatellite Analysis
2.6. Data Analyses
3. Results
3.1. Seed Traits and Growth Habit
3.2. Phaseolin (PHAS) and Phytohemagglutinin (PHA) Variability
3.3. Relationship between PHAS and PHA Patterns, and PHAS Pattern and Morphological Data
3.4. Genetic Analysis by SSR Markers
3.4.1. Genetic Diversity and Relationships among Landraces
3.4.2. Genetic Structure of Landraces
4. Discussion
4.1. Seed Morphological and Biochemical Traits of Lazio Germplasm
4.2. Molecular Characterization of Lazio Germplasm
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Cluster/Phaseolin Type | N | N Lan | N Ind | N Det | Na | Ne | Npa | AR | Ho | He |
---|---|---|---|---|---|---|---|---|---|---|
M/PHAS type S (Mesoamerican) | 84 | 10 | 10 | 0 | 3.500 | 2.212 | 10 | 3.500 | 0.010 | 0.382 |
A/PHAS type T or C (Andean) | 346 | 51 | 26 | 25 | 4.830 | 2.593 | 38 | 4.660 | 0.016 | 0.466 |
p-value Test Kruskal–Wallis | 0.199 | 0.266 | 0.174 | |||||||
M/PHAS type S (Mesoamerican) | 68 | 7 | 7 | 0 | 3.000 | 1.969 | 9 | 3.000 | 0.028 | 0.337 |
A1/PHAS type C (Andean) | 128 | 20 | 18 | 2 | 3.833 | 2.087 | 13 | 3.802 | 0.018 | 0.382 |
A2/PHAS type T (Andean) | 118 | 15 | 1 | 14 | 3.667 | 2.226 | 7 | 3.625 | 0.023 | 0.422 |
Wilcoxon Test p-value | ||||||||||
M/A1 | 0.320 | 0.220 | 0.622 | |||||||
M/A2 | 0.398 | 0.530 | 0.283 | |||||||
A1/A2 | 0.931 | 0.490 | 0.452 |
Analysis | Source | df | SS | MS | Est. Var. | % | Φ-Statistic | P(Φ) |
---|---|---|---|---|---|---|---|---|
(A) | Among groups | 1 | 1518.301 | 1518.301 | 11.149 | 50 | 0.501 | <0.001 |
Within groups | 428 | 4756.799 | 11.114 | 11.114 | 50 | |||
Total | 429 | 6275.100 | 22.263 | 100 | ||||
(B) | Among groups | 2 | 1861.247 | 930.623 | 9.091 | 50 | 0.503 | <0.001 |
Within groups | 311 | 2796.690 | 8.993 | 8.993 | 50 | |||
Total | 313 | 4657.936 | 18.084 | 100 |
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Catarcione, G.; Paolacci, A.R.; Alicandri, E.; Gramiccia, E.; Taviani, P.; Rea, R.; Costanza, M.T.; De Lorenzis, G.; Puccio, G.; Mercati, F.; et al. Genetic Diversity and Population Structure of Common Bean (Phaseolus vulgaris L.) Landraces in the Lazio Region of Italy. Plants 2023, 12, 744. https://doi.org/10.3390/plants12040744
Catarcione G, Paolacci AR, Alicandri E, Gramiccia E, Taviani P, Rea R, Costanza MT, De Lorenzis G, Puccio G, Mercati F, et al. Genetic Diversity and Population Structure of Common Bean (Phaseolus vulgaris L.) Landraces in the Lazio Region of Italy. Plants. 2023; 12(4):744. https://doi.org/10.3390/plants12040744
Chicago/Turabian StyleCatarcione, Giulio, Anna Rita Paolacci, Enrica Alicandri, Elena Gramiccia, Paola Taviani, Roberto Rea, Maria Teresa Costanza, Gabriella De Lorenzis, Guglielmo Puccio, Francesco Mercati, and et al. 2023. "Genetic Diversity and Population Structure of Common Bean (Phaseolus vulgaris L.) Landraces in the Lazio Region of Italy" Plants 12, no. 4: 744. https://doi.org/10.3390/plants12040744
APA StyleCatarcione, G., Paolacci, A. R., Alicandri, E., Gramiccia, E., Taviani, P., Rea, R., Costanza, M. T., De Lorenzis, G., Puccio, G., Mercati, F., & Ciaffi, M. (2023). Genetic Diversity and Population Structure of Common Bean (Phaseolus vulgaris L.) Landraces in the Lazio Region of Italy. Plants, 12(4), 744. https://doi.org/10.3390/plants12040744