Effects of Condensed Tannins on Bacterial and Fungal Communities during Aerobic Exposure of Sainfoin Silage
Abstract
:1. Introduction
2. Results
2.1. Characteristics of Fermentation during Aerobic Exposure in Sainfoin Silage
2.2. Bacterial Community during Aerobic Exposure
2.3. Fungal Community during Aerobic Exposure
3. Discussion
3.1. Aerobic Stability and Fermentation
3.2. Bacterial Community
3.3. Fungal Community
4. Materials and Methods
4.1. Silage Preparation
4.2. Aerobic Exposure of Silage
4.3. Characteristics Analysis of Silage
4.4. DNA Extraction and Sequence Analysis of Bacteria and Fungi
4.5. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Item | Treatment | Days of Ensiling | Days of Aerobic Exposure | SEM | p Value | |||
---|---|---|---|---|---|---|---|---|
60 | 3 | 7 | Day | Treatment | D × T | |||
DM | CK | 24.42 | 24.81 B | 24.78 B | 0.8465 | 0.0146 | <0.01 | 0.367 |
PEG | 25.42 | 26.40 A | 26.30 A | |||||
CP g/kg DM | CK | 202.93 a | 193.77 Ab | 196.02 Ab | 1.407 | <0.01 | <0.01 | <0.01 |
PEG | 202.11 a | 182.92 Bb | 183.72 Bb | |||||
pH | CK | 4.55 Ab | 4.62 Aa | 4.66 Aa | 0.011 | <0.01 | <0.01 | <0.01 |
PEG | 4.55 A | 4.54 B | 4.54 B | |||||
WSC g/kg DM | CK | 24.33 Aa | 11.80 b | 10.53 b | 1.137 | <0.01 | <0.01 | <0.01 |
PEG | 11.24 B | 7.43 | 5.26 | |||||
AN g/kg DM | CK | 0.67 Ba | 0.71 Ba | 0.32 Ab | 0.0403 | <0.01 | <0.01 | <0.01 |
PEG | 0.77 Ab | 0.94 Aa | 0.30 Ac | |||||
LA g/kg DM | CK | 23.21 a | 3.59 b | 3.66 b | 1.567 | <0.01 | 0.132 | 0.08 |
PEG | 23.67 a | 5.75 b | 3.19 b | |||||
AA g/kg DM | CK | 16.49 a | 11.41 b | 13.34 b | 0.412 | <0.01 | 0.93 | 0.457 |
PEG | 16.14 a | 12.33 b | 12.63 b | |||||
PA g/kg DM | CK | ND | ND | ND | - | - | - | - |
PEG | ND | ND | ND | |||||
BA g/kg DM | CK | ND | ND | ND | 1.153 | 0.132 | <0.01 | 0.132 |
PEG | 14.90 | 12.38 | 10.88 | |||||
Yeast Log10 CFU/g FM | CK | 2.98 Bb | 3.91a | 4.32 Aa | 0.125 | 0.01 | 0.05 | <0.01 |
PEG | 3.96 A | 4.16 | 4.08 B | |||||
LAB Log10 CFU/g FM | CK | 6.48 Bb | 6.77 Bb | 6.95 Aa | 0.1202 | 0.787 | 0.292 | 0.242 |
PEG | 7.15 Aa | 7.20 Aa | 6.63 Ab | |||||
Mold Log10 CFU/g FM | CK | 2.73 Bb | 3.08 Aa | 3.16 Aa | 0.064 | <0.01 | 0.029 | <0.01 |
PEG | 3.41 Aa | 2.68 Bb | 2.42 Bb | |||||
AB Log10 CFU/g FM | CK | 6.09 b | 6.88 b | 7.19 a | 0.106 | 0.002 | 0.937 | 0.088 |
PEG | 6.41 | 7.16 | 6.64 | |||||
Aerobic stability (h) | CK PEG | 156.32 b 182.43 a | 3.74 | - | 0.029 | - |
Days | Treatment | Ace | Chao | Shannon | Simpson | Sobs | Coverage |
---|---|---|---|---|---|---|---|
60 d of ensiling | CK | 121.03 | 114.60 | 1.78 | 0.2967 | 100.00 | 0.9995 |
PEG | 111.13 | 102.75 | 1.74 | 0.3224 | 84.60 | 0.9996 | |
3 d aerobic exposure | CK | 136.99 | 118.14 | 1.95 | 0.2403 | 90.00 | 0.9994 |
PEG | 113.79 | 92.52 | 1.70 | 0.3290 | 74.40 | 0.9994 | |
7 d aerobic exposure | CK | 149.12 | 140.47 | 2.10 | 0.2958 | 130.40 | 0.9994 |
PEG | 124.49 | 103.31 | 1.95 | 0.2416 | 82.40 | 0.9995 | |
SEM | 9.73 | 9.57 | 0.075 | 0.016 | 9.83 | - | |
p value | Treatment (T) | 0.72 | 0.78 | 0.37 | 0.59 | 0.63 | - |
Day (D) | 0.36 | 0.23 | 0.36 | 0.54 | 0.21 | - | |
T × D | 0.95 | 0.88 | 0.86 | 0.23 | 0.75 | - |
Days | Treatment | Ace | Chao | Shannon | Simpson | Sobs | Coverage |
---|---|---|---|---|---|---|---|
60 d of ensiling | CK | 189.28 a | 159.31 a | 3.00 | 0.1080 | 146.6 a | 0.9995 |
PEG | 150.36 | 146.29 a | 2.91 | 0.1067 | 134.8 a | 0.9996 | |
3 d aerobic exposure | CK | 124.47 | 119.00 | 2.56 | 0.2001 | 110.6 | 0.9994 |
PEG | 105.64 | 104.33 | 3.03 | 0.1351 | 102.0 | 0.9994 | |
7 d aerobic exposure | CK | 91.63 b | 83.55 b | 2.91 | 0.1479 | 77.0 b | 0.9994 |
PEG | 99.13 | 78.89 b | 2.68 | 0.1467 | 65.0 b | 0.9995 | |
SEM | 9.28 | 7.937 | 0.098 | 0.02 | 7.62 | - | |
p value | Treatment (T) | <0.01 | <0.01 | 0.75 | 0.53 | <0.01 | - |
Day (D) | 0.274 | 0.396 | 0.82 | 0.62 | 0.37 | - | |
T × D | 0.458 | 0.940 | 0.35 | 0.79 | 0.99 | - |
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Huang, R.; Ma, C.; Zhang, F.; Wang, X. Effects of Condensed Tannins on Bacterial and Fungal Communities during Aerobic Exposure of Sainfoin Silage. Plants 2023, 12, 2967. https://doi.org/10.3390/plants12162967
Huang R, Ma C, Zhang F, Wang X. Effects of Condensed Tannins on Bacterial and Fungal Communities during Aerobic Exposure of Sainfoin Silage. Plants. 2023; 12(16):2967. https://doi.org/10.3390/plants12162967
Chicago/Turabian StyleHuang, Rongzheng, Chunhui Ma, Fanfan Zhang, and Xuzhe Wang. 2023. "Effects of Condensed Tannins on Bacterial and Fungal Communities during Aerobic Exposure of Sainfoin Silage" Plants 12, no. 16: 2967. https://doi.org/10.3390/plants12162967