Inhibition of the CYP Enzymatic System Responsible of Heterocyclic Amines Bioactivation by an Asclepias subulata Extract
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Material
2.2. Ethanolic Extract of Asclepias subulata
2.3. Animals
2.4. Preparation of Rat Liver Microsomes
2.5. CYP1A1 and CYP1A2 Activities
2.6. Molecular Docking
2.7. Statistical Analysis
3. Results and Discussion
3.1. Inhibition of CYP1A1 by ASE (Non-Heated and Heated)
3.2. Inhibition of CYP1A2 by ASE (Non-Heated and Heated)
3.3. Molecular Docking
4. Conclusions
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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IC50 (µg/mL) | ||
---|---|---|
Asclepias subulata Extract | EROD | MROD |
Non-heated | 353.6 ± 4.51 a | 288.4 1 ± 5.81 |
Heated 2 | 75.92 ± 3.97 b | 232.1 ± 7.43 |
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Gutiérrez-Pacheco, S.L.; Peña-Ramos, E.A.; Santes-Palacios, R.; Valenzuela-Melendres, M.; Hernández-Mendoza, A.; Burgos-Hernández, A.; Robles-Zepeda, R.E.; Espinosa-Aguirre, J.J. Inhibition of the CYP Enzymatic System Responsible of Heterocyclic Amines Bioactivation by an Asclepias subulata Extract. Plants 2023, 12, 2354. https://doi.org/10.3390/plants12122354
Gutiérrez-Pacheco SL, Peña-Ramos EA, Santes-Palacios R, Valenzuela-Melendres M, Hernández-Mendoza A, Burgos-Hernández A, Robles-Zepeda RE, Espinosa-Aguirre JJ. Inhibition of the CYP Enzymatic System Responsible of Heterocyclic Amines Bioactivation by an Asclepias subulata Extract. Plants. 2023; 12(12):2354. https://doi.org/10.3390/plants12122354
Chicago/Turabian StyleGutiérrez-Pacheco, Samaria Lisdeth, Etna Aida Peña-Ramos, Rebeca Santes-Palacios, Martin Valenzuela-Melendres, Adrián Hernández-Mendoza, Armando Burgos-Hernández, Ramón Enrique Robles-Zepeda, and Jesús Javier Espinosa-Aguirre. 2023. "Inhibition of the CYP Enzymatic System Responsible of Heterocyclic Amines Bioactivation by an Asclepias subulata Extract" Plants 12, no. 12: 2354. https://doi.org/10.3390/plants12122354