Molecular Cloning and Characterization of SaCLCd, SaCLCf, and SaCLCg, Novel Proteins of the Chloride Channel Family (CLC) from the Halophyte Suaeda altissima (L.) Pall
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Material
2.2. Total RNA Extraction and First-Strand cDNA Synthesis
2.3. Amplification of SaCLCd, SaCLCf, and SaCLCg cDNA Partial Sequences
2.4. Cloning of the Full-Length SaCLCd, SaCLCf, and SaCLCg cDNA Sequences
2.5. Heterologous Expression of the SaCLCd, SaCLCf, and SaCLCg Genes in Δgef1 Yeast Mutant
2.6. Quantitative Analysis of SaCLCd, SaCLCf, and SaCLCg Transcripts in S. altissima Organs
2.7. Primer Design
2.8. Bioinformatic Analysis of Amino Acid Sequences
2.9. Determination of Chloride Content in S. altissima Organs
2.10. Statistical Analysis
3. Results
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Nedelyaeva, O.I.; Popova, L.G.; Volkov, V.S.; Balnokin, Y.V. Molecular Cloning and Characterization of SaCLCd, SaCLCf, and SaCLCg, Novel Proteins of the Chloride Channel Family (CLC) from the Halophyte Suaeda altissima (L.) Pall. Plants 2022, 11, 409. https://doi.org/10.3390/plants11030409
Nedelyaeva OI, Popova LG, Volkov VS, Balnokin YV. Molecular Cloning and Characterization of SaCLCd, SaCLCf, and SaCLCg, Novel Proteins of the Chloride Channel Family (CLC) from the Halophyte Suaeda altissima (L.) Pall. Plants. 2022; 11(3):409. https://doi.org/10.3390/plants11030409
Chicago/Turabian StyleNedelyaeva, Olga I., Larissa G. Popova, Vadim S. Volkov, and Yurii V. Balnokin. 2022. "Molecular Cloning and Characterization of SaCLCd, SaCLCf, and SaCLCg, Novel Proteins of the Chloride Channel Family (CLC) from the Halophyte Suaeda altissima (L.) Pall" Plants 11, no. 3: 409. https://doi.org/10.3390/plants11030409