Expression Profiling of Four Mango FT/TFL1-Encoding Genes under Different Fruit Load Conditions, and Their Involvement in Flowering Regulation
Abstract
:1. Introduction
2. Results
2.1. Exploring the Effects of Crop Load on Return Flowering in Mango ‘Shelly’ Cultivar
2.2. Identification and Characterization of MiFT/MiTFL1 Transcripts from Mango ‘Shelly’ Cultivar
2.3. Functional Analysis of Mango cDNA Encoding FT/TFL1 Proteins in Transgenic Arabidopsis Plants
2.4. Tissue-Specific Expression Patterns of MiFT/TFL1 Genes
2.5. Monitoring the Expression of MiFT/TFL1 Genes under Different Fruit Load Conditions
3. Discussion
4. Materials and Methods
4.1. Plant Material and De-Fruiting Treatments
4.2. Cloning of MiFT/MiTFLs from Mango ‘Shelly’ Cultivar
4.3. Arabidopsis Transformation and Phenotypic Analysis
4.4. Plant Material and Tissue Collection from Juvenile and Adult Trees
4.5. Tissue Collection from Fruit Trees with Heavy and Low Fruit Loads
4.6. RNA Isolation and cDNA Synthesis
4.7. Real-Time qPCR Analysis
4.8. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gafni, I.; Rai, A.C.; Halon, E.; Zviran, T.; Sisai, I.; Samach, A.; Irihimovitch, V. Expression Profiling of Four Mango FT/TFL1-Encoding Genes under Different Fruit Load Conditions, and Their Involvement in Flowering Regulation. Plants 2022, 11, 2409. https://doi.org/10.3390/plants11182409
Gafni I, Rai AC, Halon E, Zviran T, Sisai I, Samach A, Irihimovitch V. Expression Profiling of Four Mango FT/TFL1-Encoding Genes under Different Fruit Load Conditions, and Their Involvement in Flowering Regulation. Plants. 2022; 11(18):2409. https://doi.org/10.3390/plants11182409
Chicago/Turabian StyleGafni, Itamar, Avinash Chandra Rai, Eyal Halon, Tali Zviran, Isaac Sisai, Alon Samach, and Vered Irihimovitch. 2022. "Expression Profiling of Four Mango FT/TFL1-Encoding Genes under Different Fruit Load Conditions, and Their Involvement in Flowering Regulation" Plants 11, no. 18: 2409. https://doi.org/10.3390/plants11182409