A Small Gtp-Binding Protein GhROP3 Interacts with GhGGB Protein and Negatively Regulates Drought Tolerance in Cotton (Gossypium hirsutum L.)
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Materials and Stress Treatments
2.2. GhROP3 Bioinformatics Analysis
2.3. RNA Isolation and qRT-PCR
2.4. Construction of a Silencing Vector and VIGS in Cotton
2.5. Drought Treatment and Determination of Physiological and Biochemical Indicators
2.6. Expression Analysis of ABA and IAA Metabolism- and Signaling Pathway-Related Marker Genes after VIGS Treatment
2.7. Yeast Two-Hybrid Assay
2.8. Bimolecular Fluorescence Complementation Experiments
2.9. Statistical Analysis
3. Results
3.1. Phylogenetic Analysis of GhROP3
3.2. GhROP3 Protein Interacted with the GhGGB Protein
3.3. GhROP3 Was Expressed in Different Tissues of Cotton and Responded to Different Treatments
3.4. Silencing of the GhROP3 Gene Improved Cotton Resistance against Drought Stress
3.5. GhROP3 Negatively Regulated ABA Signaling Pathway, and Positively Regulated IAA Signaling Pathway
4. Discussion
4.1. GhROP3 Is a Prenylation Modification Substrate of GhGGB Protein
4.2. GhROP3 Played a Negative Role in the Response of Plants to Drought Stress
4.3. GhROP3 Mediated Drought Tolerance in Cotton by Participating in the Regulation of ABA and IAA Signaling Pathways
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Hu, Z.; Lei, J.; Dai, P.; Liu, C.; Wugalihan, A.; Liu, X.; Li, Y. A Small Gtp-Binding Protein GhROP3 Interacts with GhGGB Protein and Negatively Regulates Drought Tolerance in Cotton (Gossypium hirsutum L.). Plants 2022, 11, 1580. https://doi.org/10.3390/plants11121580
Hu Z, Lei J, Dai P, Liu C, Wugalihan A, Liu X, Li Y. A Small Gtp-Binding Protein GhROP3 Interacts with GhGGB Protein and Negatively Regulates Drought Tolerance in Cotton (Gossypium hirsutum L.). Plants. 2022; 11(12):1580. https://doi.org/10.3390/plants11121580
Chicago/Turabian StyleHu, Ziyao, Jianfeng Lei, Peihong Dai, Chao Liu, Abuduweili Wugalihan, Xiaodong Liu, and Yue Li. 2022. "A Small Gtp-Binding Protein GhROP3 Interacts with GhGGB Protein and Negatively Regulates Drought Tolerance in Cotton (Gossypium hirsutum L.)" Plants 11, no. 12: 1580. https://doi.org/10.3390/plants11121580