Protective Effects of Filtrates and Extracts from Fungal Endophytes on Phytophthora cinnamomi in Lupinus luteus
Abstract
:1. Introduction
2. Results
2.1. In Vitro Experiments
2.1.1. Effect of Filtrates on the Pathogen Mycelial Growth
2.1.2. Minimum Inhibitory Concentration (MIC) of Endophytic Filtrates and Extracts
2.1.3. Effect of Extracts on the Lupinus Seeds Germination
2.2. In Planta Experiments
2.2.1. Effect of the Pre-Emergence Application of Extracts on Lupinus Seedlings in Growth Chamber
2.2.2. Effect of the Post-Emergence Application of Extracts on Lupinus Seedlings in Greenhouse
3. Discussion
4. Materials and Methods
4.1. Fungal and Plant Material
4.2. Culture Conditions and Crude Extract Production from Fungal Endophytes
4.3. Effect of the Fungal Filtrates on the Pathogen Mycelial Growth In Vitro
4.4. Minimum Inhibitory Concentration In Vitro of the Filtrates and Extracts
4.5. In Planta Experiments
4.6. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Treatments | MIC | |
---|---|---|
Filtrate Dilution | Extract | |
B | 0 | 0.00 |
C | - | 2.34 |
E063 | 0 | 150.00 |
E064 | 1/2 | 300.00 |
E168 | 0 | 300.00 |
E179 | 1 | 300.00 |
Treatments | Sl (cm) 1 | Rl (cm) | Leaves # | Roots # | DMs (mg) | DMr (mg) | DMt (mg) |
---|---|---|---|---|---|---|---|
B 2 | 7.7 ± 1.0 | 5.5 ± 1.0 c | 3.5 ± 0.6 | 10.8 ± 1.0 b | 72.0 ± 7.4 | 24.8 ± 6.4 | 96.8 ± 13.5 |
C 3 | 6.3 ± 0.3 | 6.4 ± 0.1 bc | 3.0 ± 0.0 | 9.0 ± 0.5 bc | 53.0 ± 5.2 | 16.0 ± 3.8 | 69.0 ± 9.0 |
E063 | 7.9 ± 0.7 | 6.9 ± 0.9 bc | 3.8 ± 0.6 | 13.0 ± 0.7 a | 63.5 ± 4.5 | 18.3 ± 2.5 | 81.8 ± 4.9 |
E064 | 7.5 ± 0.8 | 7.7 ± 0.3 ab | 2.8 ± 0.3 | 10.0 ± 0.8 b | 62.8 ± 10.5 | 16.5 ± 5.3 | 79.3 ± 15.1 |
E168 | 5.5 ± 0.7 | 7.0 ± 0.6 abc | 3.5 ± 0.6 | 7.8 ± 0.9 c | 56.8 ± 7.9 | 12.5 ± 0.6 | 69.3 ± 7.8 |
E179 | 7.5 ± 0.5 | 8.6 ± 0.4 a | 3.0 ± 0.3 | 9.5 ± 0.3 bc | 66.0 ± 5.1 | 19.8 ± 0.3 | 85.8 ± 4.9 |
df | 5 | 5 | 5 | 5 | 5 | 5 | 5 |
F | 2.24 | 3.63 | 0.97 | 8.02 | 1.21 | 1.53 | 1.48 |
p-value | 0.0943 | 0.0191 | 0.4613 | 0.0004 | 0.3451 | 0.2316 | 0.2448 |
Treatments | AUDPC 1 | Rl (cm) | Roots # | DMs (mg) | DMr (mg) | DMt (mg) |
---|---|---|---|---|---|---|
B 2 | 0.0 ± 0.0 d | 19.6 ± 2.7 c | 14.2 ± 0.4 ab | 456.3 ± 51.8 c | 271.9 ± 26.0 bc | 728.3 ± 69.8 b |
C 3 | 168.0 ± 0.0 a | 14.7 ± 1.4 d | 6.2 ± 1.0 d | 415.4 ± 42.6 c | 242.9 ± 17.6 c | 658.3 ± 44.2 b |
E063 | 103.2 ± 5.4 b | 20.2 ± 0.6 bc | 14.6 ± 0.6 a | 427.0 ± 33.7 c | 266.7 ± 21.2 c | 693.7 ± 54.1 b |
E064 | 105.6 ± 13.0 b | 25.9 ± 0.9 a | 12.8 ± 0.4 bc | 455.6 ± 34.5 c | 259.8 ± 41.1 c | 715.4 ± 73.6 b |
E168 | 45.6 ± 10.7 c | 25.2 ± 1.9 a | 11.6 ± 0.6 c | 637.3 ± 37.9 b | 353.9 ± 51.0 ab | 991.3 ± 81.8 a |
E179 | 81.6 ± 14.3 b | 24.2 ± 0.8 ab | 13.6 ± 0.4 ab | 746.4 ± 28.2 a | 365.0 ± 20.0 a | 1111.4 ± 30.5 a |
df | 5 | 5 | 5 | 5 | 5 | 5 |
F | 47.53 | 9.44 | 32.29 | 15.35 | 3.34 | 11.56 |
p-value | <0.0001 | <0.0001 | <0.0001 | <0.0001 | 0.0197 | <0.0001 |
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García-Latorre, C.; Rodrigo, S.; Santamaria, O. Protective Effects of Filtrates and Extracts from Fungal Endophytes on Phytophthora cinnamomi in Lupinus luteus. Plants 2022, 11, 1455. https://doi.org/10.3390/plants11111455
García-Latorre C, Rodrigo S, Santamaria O. Protective Effects of Filtrates and Extracts from Fungal Endophytes on Phytophthora cinnamomi in Lupinus luteus. Plants. 2022; 11(11):1455. https://doi.org/10.3390/plants11111455
Chicago/Turabian StyleGarcía-Latorre, Carlos, Sara Rodrigo, and Oscar Santamaria. 2022. "Protective Effects of Filtrates and Extracts from Fungal Endophytes on Phytophthora cinnamomi in Lupinus luteus" Plants 11, no. 11: 1455. https://doi.org/10.3390/plants11111455